中华核医学杂志
中華覈醫學雜誌
중화핵의학잡지
CHINESE JOURNAL OF NUCLEAR MEDICINE
2011年
1期
4-8
,共5页
刘慈懿%宋少莉%谢文晖%蔡小佳%张莉华%黄钢
劉慈懿%宋少莉%謝文暉%蔡小佳%張莉華%黃鋼
류자의%송소리%사문휘%채소가%장리화%황강
肿瘤%新生血管化%A(D)A(D)APRPG%放射性核素显像%炎症%兔
腫瘤%新生血管化%A(D)A(D)APRPG%放射性覈素顯像%炎癥%兔
종류%신생혈관화%A(D)A(D)APRPG%방사성핵소현상%염증%토
Neoplasms%Neovascularization%A(D)A(D)APRPG%Radionuclide imaging%Inflammation%Rabbits
目的 将抗肿瘤血管化显像剂99Tcm-联肼尼克酰胺-非天然型丙氨酸-非天然型丙氨酸-天然型丙氨酸-天然型脯氨酸-天然型精氨酸-天然型脯氨酸-天然型甘氨酸[HYNIC-A(D)A(D)APRPG]进行肿瘤与炎性反应的动物实验研究,评估其显像特异性.方法 分别在10只新西兰兔左、右上肢建立炎性反应和肿瘤模型,按完全随机设计法将兔分为2组,每组分别静脉注射99Tcm-HYNIC-A(D)A(D)APRPG 与99TcmRGD,分别在0.5,1,2,3,6和8 h进行γ显像;前一组5只兔先进行18F-FDG PET/CT显像,24 h后再注射99Tcm-HYNIC-A(D)A(D)APPPG进行显像.采用SPSS 10.0软件对数据行方差分析或t检验.结果 99Tcm-HYNIC-A(D)A(D)APRPG显像只显示肿瘤组织清晰致密、血供丰富的区域,坏死区域不显影;炎性反应区域基本不显影.99Tcm-HYNIC-A(D)A(D)APRPG注射后2 h肿瘤/炎性反应比值最高,为3.25±0.171,高于99Tcm-RGD的2.37±0.076(F=15.63,P<0.01);在0.5～6 h99Tcm-HYNIC-A(D)A(D)APRPG和99TcmRGD、18F-FDG的肿瘤/炎性反应比值组间差异均有统计学意义(F:13.83～26.41,t=23.84和12.75,P均<0.01).结论 99Tcm-HYNIC-A(D)A(D)APRPG有望成为一种能区分肿瘤和无菌性炎性反应的抗肿瘤血管化显像剂.
目的 將抗腫瘤血管化顯像劑99Tcm-聯肼尼剋酰胺-非天然型丙氨痠-非天然型丙氨痠-天然型丙氨痠-天然型脯氨痠-天然型精氨痠-天然型脯氨痠-天然型甘氨痠[HYNIC-A(D)A(D)APRPG]進行腫瘤與炎性反應的動物實驗研究,評估其顯像特異性.方法 分彆在10隻新西蘭兔左、右上肢建立炎性反應和腫瘤模型,按完全隨機設計法將兔分為2組,每組分彆靜脈註射99Tcm-HYNIC-A(D)A(D)APRPG 與99TcmRGD,分彆在0.5,1,2,3,6和8 h進行γ顯像;前一組5隻兔先進行18F-FDG PET/CT顯像,24 h後再註射99Tcm-HYNIC-A(D)A(D)APPPG進行顯像.採用SPSS 10.0軟件對數據行方差分析或t檢驗.結果 99Tcm-HYNIC-A(D)A(D)APRPG顯像隻顯示腫瘤組織清晰緻密、血供豐富的區域,壞死區域不顯影;炎性反應區域基本不顯影.99Tcm-HYNIC-A(D)A(D)APRPG註射後2 h腫瘤/炎性反應比值最高,為3.25±0.171,高于99Tcm-RGD的2.37±0.076(F=15.63,P<0.01);在0.5～6 h99Tcm-HYNIC-A(D)A(D)APRPG和99TcmRGD、18F-FDG的腫瘤/炎性反應比值組間差異均有統計學意義(F:13.83～26.41,t=23.84和12.75,P均<0.01).結論 99Tcm-HYNIC-A(D)A(D)APRPG有望成為一種能區分腫瘤和無菌性炎性反應的抗腫瘤血管化顯像劑.
목적 장항종류혈관화현상제99Tcm-련정니극선알-비천연형병안산-비천연형병안산-천연형병안산-천연형포안산-천연형정안산-천연형포안산-천연형감안산[HYNIC-A(D)A(D)APRPG]진행종류여염성반응적동물실험연구,평고기현상특이성.방법 분별재10지신서란토좌、우상지건립염성반응화종류모형,안완전수궤설계법장토분위2조,매조분별정맥주사99Tcm-HYNIC-A(D)A(D)APRPG 여99TcmRGD,분별재0.5,1,2,3,6화8 h진행γ현상;전일조5지토선진행18F-FDG PET/CT현상,24 h후재주사99Tcm-HYNIC-A(D)A(D)APPPG진행현상.채용SPSS 10.0연건대수거행방차분석혹t검험.결과 99Tcm-HYNIC-A(D)A(D)APRPG현상지현시종류조직청석치밀、혈공봉부적구역,배사구역불현영;염성반응구역기본불현영.99Tcm-HYNIC-A(D)A(D)APRPG주사후2 h종류/염성반응비치최고,위3.25±0.171,고우99Tcm-RGD적2.37±0.076(F=15.63,P<0.01);재0.5～6 h99Tcm-HYNIC-A(D)A(D)APRPG화99TcmRGD、18F-FDG적종류/염성반응비치조간차이균유통계학의의(F:13.83～26.41,t=23.84화12.75,P균<0.01).결론 99Tcm-HYNIC-A(D)A(D)APRPG유망성위일충능구분종류화무균성염성반응적항종류혈관화현상제.
Objective To investigate the uptake of 99Tcm-hydrazinonicotinamide-D-alanine-D-alanine-alanine-proline-arginine-proline-glycine (HYNIC-A(D) A(D) APRPG) in rabbit models of inflammation and VX2 tumor xenografted, so as to evaluate its use as a new tracer for tumor angiogenesis. Methods Ten rabbit models of xenoplanted VX2 tumor and inflammation were randomly divided into two groups which were injected with different injected tracers, 99Tcm-HYNIC-A(D) A (D)APRPG 99Tcm-RGD, followed by serial Gamma images at various time points. The first group underwent 18F-FDG PET ahead of 99Tcm-HYNICA(D)A (D) APRPG SPECT. Analysis of variance and t-test were performed with SPSS 10.0. Results 99TcmHYNIC-A(D) A (D)APRPG scan showed negative uptake at inflammation focus but positive uptake at tumor. Pathological examination confirmed high 99Tcm-HYNIC-A(D)A(D) APRPG accumulation in tumor cells, with the highest tumor/inflammation ratio (3.25 ±0. 171) at 2 h post-injection, which was significantly higher than that of 99Tcm-RGD (2.37 ± 0.076) (F = 15. 63, P<0. 01). The tumor/inflammation ratios of 99Tcm-HYNIC-A(D)A(D)APRPG, 99Tcm-RGD, 18F-FDG were significantly different at 0.5, 1,2,3, 6 h (F = 13. 83～26. 41; t = 23.84, 12.75; all P<0. 01). Conclusion 99Tcm-HYNIC-A (D) A (D)APRPG can be used as a potential tracer for tumor angiogenesis.
