中华放射医学与防护杂志
中華放射醫學與防護雜誌
중화방사의학여방호잡지
Chinese Journal of Radiological Medicine and Protection
2011年
6期
653-656
,共4页
杨爱洁%何信佳%安永恒%王鑫
楊愛潔%何信佳%安永恆%王鑫
양애길%하신가%안영항%왕흠
辐射效应%放射损伤%胰腺
輻射效應%放射損傷%胰腺
복사효응%방사손상%이선
Radiation effects%Radiation injuries%Pancreas
目的 探讨不同分割模式X射线照射后大鼠胰腺的损伤程度.方法 将健康成年Wistar大鼠80只,随机分为常规分割组,12 Gy分6次照射,每天1次；单次大剂量组,一次性照射12 Gy；中等低分割组,12 Gy分2次照射,中间间隔4d；对照组,给予假照；每组20只.于照射前及照射后4、7、14、21和42 d称重、测空腹血糖、淀粉酶,观察胰腺组织学改变.结果 各实验组血糖照射后4d时降至最低,最低时达(3.1±0.1)mmol/L,(LSD-t=20.06～28.74,P＜0.001),单次大剂量组较常规分割组、中等低分割组降低更明显(LSD-t =8.72～9.71,P＜0.001),常规分割组、中等低分割组差异无统计学意义,14 d及以后接近正常；血淀粉酶照射后4和7d时升高,最高时可达(84.5±6.4 )U/L(Dunnett's-t =23.10～46.10,P＜0.001),7d时单次大剂量组较常规分割组、中等低分割组升高更明显(Dunnett's-t=7.11,P＜0.05),常规分割组与中等低分割组比较差异无统计学意义,14d及以后恢复正常.光镜下,单次大剂量组4d时个别腺泡变性坏死,14 d时腺管周围间质纤维增生,21 d时胰岛细胞间纤维增生,42 d时腺细胞增生.常规分割组、中等低分割组出现同样变化,仅程度较轻,出现较晚.结论 当分次剂量及间隔时间合适时,中等低分割照射损伤程度未超过常规照射,这对临床非常规放疗的开展有一定的参考价值.
目的 探討不同分割模式X射線照射後大鼠胰腺的損傷程度.方法 將健康成年Wistar大鼠80隻,隨機分為常規分割組,12 Gy分6次照射,每天1次；單次大劑量組,一次性照射12 Gy；中等低分割組,12 Gy分2次照射,中間間隔4d；對照組,給予假照；每組20隻.于照射前及照射後4、7、14、21和42 d稱重、測空腹血糖、澱粉酶,觀察胰腺組織學改變.結果 各實驗組血糖照射後4d時降至最低,最低時達(3.1±0.1)mmol/L,(LSD-t=20.06～28.74,P＜0.001),單次大劑量組較常規分割組、中等低分割組降低更明顯(LSD-t =8.72～9.71,P＜0.001),常規分割組、中等低分割組差異無統計學意義,14 d及以後接近正常；血澱粉酶照射後4和7d時升高,最高時可達(84.5±6.4 )U/L(Dunnett's-t =23.10～46.10,P＜0.001),7d時單次大劑量組較常規分割組、中等低分割組升高更明顯(Dunnett's-t=7.11,P＜0.05),常規分割組與中等低分割組比較差異無統計學意義,14d及以後恢複正常.光鏡下,單次大劑量組4d時箇彆腺泡變性壞死,14 d時腺管週圍間質纖維增生,21 d時胰島細胞間纖維增生,42 d時腺細胞增生.常規分割組、中等低分割組齣現同樣變化,僅程度較輕,齣現較晚.結論 噹分次劑量及間隔時間閤適時,中等低分割照射損傷程度未超過常規照射,這對臨床非常規放療的開展有一定的參攷價值.
목적 탐토불동분할모식X사선조사후대서이선적손상정도.방법 장건강성년Wistar대서80지,수궤분위상규분할조,12 Gy분6차조사,매천1차；단차대제량조,일차성조사12 Gy；중등저분할조,12 Gy분2차조사,중간간격4d；대조조,급여가조；매조20지.우조사전급조사후4、7、14、21화42 d칭중、측공복혈당、정분매,관찰이선조직학개변.결과 각실험조혈당조사후4d시강지최저,최저시체(3.1±0.1)mmol/L,(LSD-t=20.06～28.74,P＜0.001),단차대제량조교상규분할조、중등저분할조강저경명현(LSD-t =8.72～9.71,P＜0.001),상규분할조、중등저분할조차이무통계학의의,14 d급이후접근정상；혈정분매조사후4화7d시승고,최고시가체(84.5±6.4 )U/L(Dunnett's-t =23.10～46.10,P＜0.001),7d시단차대제량조교상규분할조、중등저분할조승고경명현(Dunnett's-t=7.11,P＜0.05),상규분할조여중등저분할조비교차이무통계학의의,14d급이후회복정상.광경하,단차대제량조4d시개별선포변성배사,14 d시선관주위간질섬유증생,21 d시이도세포간섬유증생,42 d시선세포증생.상규분할조、중등저분할조출현동양변화,부정도교경,출현교만.결론 당분차제량급간격시간합괄시,중등저분할조사손상정도미초과상규조사,저대림상비상규방료적개전유일정적삼고개치.
Objective To investigate the damages on pancreas after different fractionated irradiation in rats.Methods Eighy healthy adult Wistar rats were randomly divided into four groups with 20 rats in each group as conventional fractionated irradiation group with 2 Gy per fraction to a dose of 12 Gy,hypofractionated radiation group with one fraction of 12 Gy,middle-dose fractionated radiation group with 6 Gy per fraction to a dose of 12 Gy in the interval of 4 days and control group without radiation.Changes in weight,fasting blood glucose and amylase were measured and morphological changes were observed in different periods.Results In the experimental groups,the reduction was observed in fasting glucose at 4 d,reached a minimum of (3.1 ±0.1 ) mmol/L,(LSD-t =20.06 -28.74,P ＜0.001 ) and the increase of amylase was found after 4th and 7th day,reached a maximum of (84.5 ±6.4) U/L(Dunnett's-t=23.10 -46.10,P ＜ 0.001 ),both more obvious in hypofractionated radiation group than those of conventional fractionated radiation group and middle-dose fractionated radiation group ( LSD-t =8.72-9.71,Dunnett's-t =7.11,P ＜ 0.05 ),however the levels in conventional fractionated radiation group was nearly to middle-dose fractionated radiation group (P ＞ 0.05 ) and became normal at 14 d.Under light microscope,the necrosis of acinarcells was observed in hypofractionated radiation group at 4th d,interstitial fibrogenesis were found at 14 d,the fibrogenesis were found in pancreatic island at 21 d,and the hyperplasia of acinarcells was observed at 42 d.The same changes were found in conventional fractionated radiation group and middle-dose fractionated radiation group,which were gently and lately than those of hypofractionated radiation group.Conclusions Radiation injury is not more serious after middle-dose fractionated radiotherapy than that after conventional fractionated irradiation,when the proper fractional dose and intervals are chosen.
