CAJ | 학술논문

目的研究湖北地区宫颈癌患者人乳头状瘤病毒16型(HPV16)E6基因点突变分布及频率,与国内外其他研究人员发现的E6突变进行对比,分析E6突变在宫颈癌发生中的意义.方法从宫颈癌患者手术切除标本中提取组织DNA,用HPV16 E6特异性引物进行PCR扩增,对扩增的E6基因片段进行测序分析.结果在35例宫颈癌组织DNA中有18例发生E6基因178位核苷酸的突变,变突频率为51.43%,相应核苷酸改变为Asp→Glu,442位核苷酸有4例发生核苷酸序列的改变,突变频率为11.43%,另有10例发生1～2个核苷酸序列的改变.结论湖北地区98例宫颈癌患者人乳头状瘤病毒E6基因存在高频率的178和442位核苷酸突变,一些为新发现的核苷酸序列的突变,这些突变在宫颈癌发生中的作用有待于进一步研究.
목적 연구호북지구궁경암환자인유두상류병독16형(HPV16)E6기인점돌변분포급빈솔,여국내외기타연구인원발현적E6돌변진행대비,분석E6돌변재궁경암발생중적의의.방법 종궁경암환자수술절제표본중제취조직DNA,용HPV16 E6특이성인물진행PCR확증,대확증적E6기인편단진행측서분석.결과 재35례궁경암조직DNA중유18례발생E6기인178위핵감산적돌변,변돌빈솔위51.43%,상응핵감산개변위Asp→Glu,442위핵감산유4례발생핵감산서렬적개변,돌변빈솔위11.43%,령유10례발생1～2개핵감산서렬적개변.결론 호북지구98례궁경암환자인유두상류병독E6기인존재고빈솔적178화442위핵감산돌변,일사위신발현적핵감산서렬적돌변,저사돌변재궁경암발생중적작용유대우진일보연구.
Objective To study the mutation of papillomavirus type 16 (HPV16) E6 gene, and to compare with the result of other researchers. Methods DNA was extracted from cervical cancer tissue sam-pies, then HPV16 E6 genes were amplified and sequenced using the HPV16 E6 specific primers from cervi-cal cancer DNA samples. Results HPV16 E6 gene mutations at the position of nt178 were found in 18 case from 35 cervical cancer samples, the mutation rate was 51.43%, resulting in nucleotide change of Asp to Glu. E6 mutations are found at the position of nt442 in 4 cervical samples. E6 mutation was found in 1 to 2 case in other nucleotide. Conclusion High frequency mutations of E6 gene of HPV16 at the position of nt178, nt442 have been found in cervical cancer samples in Hubei, China. The role of E6 gene mutation in cervical cancer development should be studied further.