CAJ | 학술논문

为探讨HIV－1gp41N端融合肽诱导膜融合的机理，利用傅里叶变换红外光谱技术研究了化学方法合成的代表HIV－1gp41N末端的23肽（HIVWT）分别与酸性POPG和中性POPC脂膜作用后，多肽H/D交换程度的变化及其在膜中的取向。结果表明，HIVWT分子中的大部份氨基酸残基被POPG脂膜保护，分子骨架上的酰胺质子只有11.9％可被D交换，而HIVWT被POPC脂膜保护的程度则小得多；在POPG脂质体中，HIVWT分子中的α－螺旋与脂双层平面的角度为29±2°，β－折叠与脂双层平面的夹角为25±1°。在POPC脂质体中，HIVWT分子的α－螺旋与脂双层平面的角度为22±1°;β－折叠与脂双层平面的夹角为26±3°。根据实验结果，对HIVWT的插膜状态进行了讨论。
위탐토HIV－1gp41N단융합태유도막융합적궤리，이용부리협변환홍외광보기술연구료화학방법합성적대표HIV－1gp41N말단적23태（HIVWT）분별여산성POPG화중성POPC지막작용후，다태H/D교환정도적변화급기재막중적취향。결과표명，HIVWT분자중적대부빈안기산잔기피POPG지막보호，분자골가상적선알질자지유11.9％가피D교환，이HIVWT피POPC지막보호적정도칙소득다；재POPG지질체중，HIVWT분자중적α－라선여지쌍층평면적각도위29±2°，β－절첩여지쌍층평면적협각위25±1°。재POPC지질체중，HIVWT분자적α－라선여지쌍층평면적각도위22±1°;β－절첩여지쌍층평면적협각위26±3°。근거실험결과，대HIVWT적삽막상태진행료토론。
To investigate the mechanism of membrane fusion induced by HIV-1gp41 N terminal fusion peptide,we studied its membrane insertion state and orientation by H/D exchange and polarized FTIR in acidic POPG and neutral POPC liposomes,respectively.The results indicate that only 11.9％ of backbone amide protons of HIVWT in POPG vesicles undergo H/D exchange,whereas 44.6％ amide protons are exchangeable for HIVWT in POPC SUV.The average angles of α -helix and β -strand of HIVWT with respect to the POPG bilayer surface are 29± 2° and 25± 1° ,respectively.In the presence of POPC SUV,the average angle between the α -helix of HIVWT and the bilayer surface is 22± 1° ,and the average angle of β -strand of HIVWT is 26± 3° .The membrane insertion state of HIVWT was discussed.