河南医科大学学报
河南醫科大學學報
하남의과대학학보
JOURNAL OF HENAN MEDICAL UNIVERSITY
2001年
3期
254-256
,共3页
王建祯%付瑞锋%张鹏远%宋乐%臧卫东%牛光明
王建禎%付瑞鋒%張鵬遠%宋樂%臧衛東%牛光明
왕건정%부서봉%장붕원%송악%장위동%우광명
血管内皮生长因子%RNA,反义%转染%垂体瘤
血管內皮生長因子%RNA,反義%轉染%垂體瘤
혈관내피생장인자%RNA,반의%전염%수체류
目的:研究血管内皮生长因子(VEGF)反义RNA对人脑垂体瘤细胞的抑制作用,探讨以VEGF反义RNA进行垂体瘤基因治疗的可行性。方法:将VEGF反义cDNA、正义VEGF cDNA质粒转染垂体瘤细胞,通过Northern印迹杂交鉴定其表达,测定被转染细胞生长率,用原位杂交和免疫组化法检测VEGF mRNA及蛋白表达。结果:经鉴定被转染的垂体瘤细胞有外源性VEGF反义基因的整合及表达,其VEGF mRNA及蛋白表达水平降低,但其生长速率无明显减慢。结论:VEGF在垂体瘤血管形成及发生、发展中起重要作用,可能成为基因治疗垂体瘤的优选靶的之一。
目的:研究血管內皮生長因子(VEGF)反義RNA對人腦垂體瘤細胞的抑製作用,探討以VEGF反義RNA進行垂體瘤基因治療的可行性。方法:將VEGF反義cDNA、正義VEGF cDNA質粒轉染垂體瘤細胞,通過Northern印跡雜交鑒定其錶達,測定被轉染細胞生長率,用原位雜交和免疫組化法檢測VEGF mRNA及蛋白錶達。結果:經鑒定被轉染的垂體瘤細胞有外源性VEGF反義基因的整閤及錶達,其VEGF mRNA及蛋白錶達水平降低,但其生長速率無明顯減慢。結論:VEGF在垂體瘤血管形成及髮生、髮展中起重要作用,可能成為基因治療垂體瘤的優選靶的之一。
목적:연구혈관내피생장인자(VEGF)반의RNA대인뇌수체류세포적억제작용,탐토이VEGF반의RNA진행수체류기인치료적가행성。방법:장VEGF반의cDNA、정의VEGF cDNA질립전염수체류세포,통과Northern인적잡교감정기표체,측정피전염세포생장솔,용원위잡교화면역조화법검측VEGF mRNA급단백표체。결과:경감정피전염적수체류세포유외원성VEGF반의기인적정합급표체,기VEGF mRNA급단백표체수평강저,단기생장속솔무명현감만。결론:VEGF재수체류혈관형성급발생、발전중기중요작용,가능성위기인치료수체류적우선파적지일。
Aim:To study the inhibitory effect of VEGF antisense RNA on the growth of human pituitary adenoma cells in vitro, in order to further investigate the feasibility of VEGF antisense RNA gene therapy of pituitary adenomas. Methods:Carrying with VEGF antisense cDNA, VEGF sense cDNA plasmid was transfected into pituitary adenoma cells, and confirmed its expression by Northern blot.The survival rate of transfected cells was detected by MTT assay.The expression of VEGF mRNA and protein was detected by Northern blot analysis, in situ hybridization and immunohistochemistry. Results:The expression of exogenous antisense VEGF mRNA was confirmed in transfected cells, and the endogenous VEGF mRNA and VEGF protein level was dramatically decreased. Conclusion: VEGF may play an important role in angiogenesis and development of pituitary adenoma, and suggests that VEGF gene is a target for gene therapy of pituitary adenoma.