中国临床康复
中國臨床康複
중국림상강복
CHINESE JOURNAL OF CLINICAL REHABILITATION
2005年
38期
163-165
,共3页
骨肉瘤%蛋白质p53%基因,bcl-2%增殖细胞核抗原%免疫组织化学
骨肉瘤%蛋白質p53%基因,bcl-2%增殖細胞覈抗原%免疫組織化學
골육류%단백질p53%기인,bcl-2%증식세포핵항원%면역조직화학
背景:骨肉瘤在好发于轻少年恶性骨与软组织的肿瘤中居第一位,在临床中,发现新疆少数民族中的发病率较为多见,其发病和预后在基因水平是否存在种族差异.目的:观察新疆不同民族骨肉瘤发生发展中的p53,bcl-2,增殖细胞核抗原基因表达差异情况.设计:非随机临床病理标本对照的实验.单位:新疆医科大学病理中心免疫组织化学实验室.对象:取自新疆医科大学第一附属医院及石河子大学第一附属医院病理科1984-01-01/2001-12-31手术切除的52例骨肉瘤患者组织标本(52块),男29例,女23例;其中哈族12例,维族17例,汉族23例);另取32例瘤样病变患者组织(骨纤维结构不良,纤维异常增殖症)标本(32块),其中哈族7例,维族11例,汉族14例.所取标本患者均获完全知情同意将自己的标本用于此项研究.方法:实验在新疆医科大学病理中心免疫组织化学实验室进行.两种标本中p53,bcl-2和增殖细胞核抗原表达监测应用免疫组织化学ISAB法.以磷酸盐缓冲液代替一抗作空白对照,以已知阳性的肿瘤标本作阳性对照.P53蛋白和增殖细胞核抗原阳性表达在细胞核内,有明显的红棕色颗粒状染色为阳性,而Bcl-2蛋白阳性则定位于胞浆上.主要观察指标:①不同民族骨肉瘤中p53,bcl-2和增殖细胞核抗原的表达结果.②p53,bcl-2,增殖细胞核抗原在骨肉瘤和骨的瘤样病变组织的表达情况.③骨肉瘤中bcl-2与p53和增殖细胞核抗原间的相关性.结果:①不同民族骨肉瘤中p53,bcI-2和增殖细胞核抗原的表达结果:哈族、维族及汉各之间p53,bcl-2,增殖细胞核抗原阳性率无显著差别(P＞0.05).②p53,bcl-2,增殖细胞核抗原在骨肉瘤和骨的瘤样病变组织的表达情况:骨肉瘤中P53,Bcl-2蛋白、增殖细胞核抗原表达阳性率均显著高于瘤样病变组织(42.31%比3.13%,59.62%比0.75%比31.25%,P＜0.01).③骨肉瘤中bcl-2与p53和增殖细胞核抗原间的相关性:骨肉瘤中bcl-2与p53之间、bcl-2与增殖细胞核抗原之间有相关关系(X2=5.8182,4.9000,P＜0.05).结论:结果表明骨肉瘤组织内p53,bcl-2和增殖细胞核抗原表达与肿瘤分化程度,民族分布未见统计学差别,说明这些基因在骨肉肿瘤发生过程中具有共同调控环节,与不同民族的遗传背景关系不大.
揹景:骨肉瘤在好髮于輕少年噁性骨與軟組織的腫瘤中居第一位,在臨床中,髮現新疆少數民族中的髮病率較為多見,其髮病和預後在基因水平是否存在種族差異.目的:觀察新疆不同民族骨肉瘤髮生髮展中的p53,bcl-2,增殖細胞覈抗原基因錶達差異情況.設計:非隨機臨床病理標本對照的實驗.單位:新疆醫科大學病理中心免疫組織化學實驗室.對象:取自新疆醫科大學第一附屬醫院及石河子大學第一附屬醫院病理科1984-01-01/2001-12-31手術切除的52例骨肉瘤患者組織標本(52塊),男29例,女23例;其中哈族12例,維族17例,漢族23例);另取32例瘤樣病變患者組織(骨纖維結構不良,纖維異常增殖癥)標本(32塊),其中哈族7例,維族11例,漢族14例.所取標本患者均穫完全知情同意將自己的標本用于此項研究.方法:實驗在新疆醫科大學病理中心免疫組織化學實驗室進行.兩種標本中p53,bcl-2和增殖細胞覈抗原錶達鑑測應用免疫組織化學ISAB法.以燐痠鹽緩遲液代替一抗作空白對照,以已知暘性的腫瘤標本作暘性對照.P53蛋白和增殖細胞覈抗原暘性錶達在細胞覈內,有明顯的紅棕色顆粒狀染色為暘性,而Bcl-2蛋白暘性則定位于胞漿上.主要觀察指標:①不同民族骨肉瘤中p53,bcl-2和增殖細胞覈抗原的錶達結果.②p53,bcl-2,增殖細胞覈抗原在骨肉瘤和骨的瘤樣病變組織的錶達情況.③骨肉瘤中bcl-2與p53和增殖細胞覈抗原間的相關性.結果:①不同民族骨肉瘤中p53,bcI-2和增殖細胞覈抗原的錶達結果:哈族、維族及漢各之間p53,bcl-2,增殖細胞覈抗原暘性率無顯著差彆(P＞0.05).②p53,bcl-2,增殖細胞覈抗原在骨肉瘤和骨的瘤樣病變組織的錶達情況:骨肉瘤中P53,Bcl-2蛋白、增殖細胞覈抗原錶達暘性率均顯著高于瘤樣病變組織(42.31%比3.13%,59.62%比0.75%比31.25%,P＜0.01).③骨肉瘤中bcl-2與p53和增殖細胞覈抗原間的相關性:骨肉瘤中bcl-2與p53之間、bcl-2與增殖細胞覈抗原之間有相關關繫(X2=5.8182,4.9000,P＜0.05).結論:結果錶明骨肉瘤組織內p53,bcl-2和增殖細胞覈抗原錶達與腫瘤分化程度,民族分佈未見統計學差彆,說明這些基因在骨肉腫瘤髮生過程中具有共同調控環節,與不同民族的遺傳揹景關繫不大.
배경:골육류재호발우경소년악성골여연조직적종류중거제일위,재림상중,발현신강소수민족중적발병솔교위다견,기발병화예후재기인수평시부존재충족차이.목적:관찰신강불동민족골육류발생발전중적p53,bcl-2,증식세포핵항원기인표체차이정황.설계:비수궤림상병리표본대조적실험.단위:신강의과대학병리중심면역조직화학실험실.대상:취자신강의과대학제일부속의원급석하자대학제일부속의원병이과1984-01-01/2001-12-31수술절제적52례골육류환자조직표본(52괴),남29례,녀23례;기중합족12례,유족17례,한족23례);령취32례류양병변환자조직(골섬유결구불량,섬유이상증식증)표본(32괴),기중합족7례,유족11례,한족14례.소취표본환자균획완전지정동의장자기적표본용우차항연구.방법:실험재신강의과대학병리중심면역조직화학실험실진행.량충표본중p53,bcl-2화증식세포핵항원표체감측응용면역조직화학ISAB법.이린산염완충액대체일항작공백대조,이이지양성적종류표본작양성대조.P53단백화증식세포핵항원양성표체재세포핵내,유명현적홍종색과립상염색위양성,이Bcl-2단백양성칙정위우포장상.주요관찰지표:①불동민족골육류중p53,bcl-2화증식세포핵항원적표체결과.②p53,bcl-2,증식세포핵항원재골육류화골적류양병변조직적표체정황.③골육류중bcl-2여p53화증식세포핵항원간적상관성.결과:①불동민족골육류중p53,bcI-2화증식세포핵항원적표체결과:합족、유족급한각지간p53,bcl-2,증식세포핵항원양성솔무현저차별(P＞0.05).②p53,bcl-2,증식세포핵항원재골육류화골적류양병변조직적표체정황:골육류중P53,Bcl-2단백、증식세포핵항원표체양성솔균현저고우류양병변조직(42.31%비3.13%,59.62%비0.75%비31.25%,P＜0.01).③골육류중bcl-2여p53화증식세포핵항원간적상관성:골육류중bcl-2여p53지간、bcl-2여증식세포핵항원지간유상관관계(X2=5.8182,4.9000,P＜0.05).결론:결과표명골육류조직내p53,bcl-2화증식세포핵항원표체여종류분화정도,민족분포미견통계학차별,설명저사기인재골육종류발생과정중구유공동조공배절,여불동민족적유전배경관계불대.
BACKGROUND: Osteosarcoma is the most common type of bone tumor and generally occurs between the age of 10 to 25; moreover, in clinical practice osteosarcoma is found to occur more often in minority nationalities in Xinjiang Autonomous Region. Do its incidence and prognosis vary between nationalities at gene levelOBJECTIVE: To observe the different expressions of p53, bcl-2 and proliferative cell nucleus antigen (PCNA) gene during the development of osteosarcoma between various Xinjiang nationalities.DESIGN: Non-randomized comparative experiment taking clinical pathological specimens as subjects.SETTING: Immunohistochemical Laboratory, the Pathological Center of Xinjiang Medical University.PARTICIPANTS: Totally 52 pieces of specimen were obtained from excised osteosarcoma tissues in the Pathological Department, the First Affiliated Hospital of Xinjiang Medical University, and the Pathological Department, the First Affiliated Hospital of Shihezi University, between January 1,1984 and December 31, 2001. The 52 cases of osteosarcoma included 29cases derived from male patients and 23 from female patients; among them there were 12 cases of Kazak minority, 17 cases of Uygur minority and 23cases of Han nationality. Other 32 specimens were obtained from 32 patients with tumor-like lesions (such as osteofibrous dysplasia or fibrous dysplasia), including 7 cases of Kazak minority, 11 cases of Uygur minority,and 14 cases of Han nationality. The informed consent was obtained from the patients.METHODS: This experiment was carried out at the Immunohistochemical Laboratory, the Pathological Center of Xinjiang Medical University. LSAB method was used to detect p53, bcl-2 and PCNA expression in the two groups. The first antibody was re placed by PBS as blank control, and the available positive expression was taken as positive control. P53 protein and PCNA were observed to express in cell nucleus, appearing obvious redbrown granules with positive expression, whereas Bcl-2 protein was expressed in cytoplasm.lationship between the expression of Bcl-2, p53 and PCNA in osteosarcoma.of different nationalities: The expression of p53, bcl-2 and PCNA in Kazak minority, Uygur minority and Han nationality was not remarkable p53, bcl-2 and PCNA in osteosarcoma and bone tumor-like lesions: The expression of P53, Bcl-2 protein and PCNA in osteosarcoma was remarkably higher than that in tumor-like lesions (42.31% vs. 3.13 %, 59.62%sion of bcl-2, p53 and PCNA in osteosarcoma: There was a close correlation between bcl-2 and p53, as well as between bcl-2 and PCNA in osteosarcoma tissues (X2 =5.818 2, 4.900 0, P＜0.05).CONCLUSION: The results indicate that there was no statistical difference between various nationalities in the expression of p53, bcl-2 and PCNA, as well as osteosarcoma differentiation. Suggesting that these genes may share the common regulation during the development of osteosarcoma,which is less associated with their nationality-related hereditary background.
