中国临床保健杂志
中國臨床保健雜誌
중국림상보건잡지
CHINESE JOURNAL OF CLINICAL HEALTHCARE
2009年
3期
273-275,封3
,共4页
结肠炎,溃疡性%趋化因子CCL2%毒力
結腸炎,潰瘍性%趨化因子CCL2%毒力
결장염,궤양성%추화인자CCL2%독력
Colitis,ulcerative%Chemokine CCL2%Virulence
目的 探讨单核细胞趋化蛋白-1(MCP-1)在溃疡性结肠炎(UC)发病机制中的作用.方法 32例UC黏膜标本取自行结肠镜检查的患者,按病理组织学对炎症进行分级,Ⅲ、Ⅳ级20 例,Ⅰ、Ⅱ级 12例;对照组为15名健康成人.应用半定量RT-PCR检测对照组和UC组肠黏膜MCP-1 mRNA表达水平,免疫组织化学方法检测MCP-1蛋白的表达.结果 与对照组相比,UC肠黏膜MCP-1 mRNA和其蛋白过度表达,MCP-1 mRNA表达与疾病严重程度成正相关(P<0.01).MCP-1蛋白表达位于UC肠黏膜固有层单核细胞和T淋巴细胞,病理分级Ⅲ、Ⅳ级肠黏膜MCP-1蛋白表达较病理分级Ⅰ、Ⅱ级组明显增加(t=7.31,P<0.01).结论 UC黏膜组织中MCP-1表达水平明显上调,其表达水平与病情轻重和炎症严重程度呈正相关.
目的 探討單覈細胞趨化蛋白-1(MCP-1)在潰瘍性結腸炎(UC)髮病機製中的作用.方法 32例UC黏膜標本取自行結腸鏡檢查的患者,按病理組織學對炎癥進行分級,Ⅲ、Ⅳ級20 例,Ⅰ、Ⅱ級 12例;對照組為15名健康成人.應用半定量RT-PCR檢測對照組和UC組腸黏膜MCP-1 mRNA錶達水平,免疫組織化學方法檢測MCP-1蛋白的錶達.結果 與對照組相比,UC腸黏膜MCP-1 mRNA和其蛋白過度錶達,MCP-1 mRNA錶達與疾病嚴重程度成正相關(P<0.01).MCP-1蛋白錶達位于UC腸黏膜固有層單覈細胞和T淋巴細胞,病理分級Ⅲ、Ⅳ級腸黏膜MCP-1蛋白錶達較病理分級Ⅰ、Ⅱ級組明顯增加(t=7.31,P<0.01).結論 UC黏膜組織中MCP-1錶達水平明顯上調,其錶達水平與病情輕重和炎癥嚴重程度呈正相關.
목적 탐토단핵세포추화단백-1(MCP-1)재궤양성결장염(UC)발병궤제중적작용.방법 32례UC점막표본취자행결장경검사적환자,안병리조직학대염증진행분급,Ⅲ、Ⅳ급20 례,Ⅰ、Ⅱ급 12례;대조조위15명건강성인.응용반정량RT-PCR검측대조조화UC조장점막MCP-1 mRNA표체수평,면역조직화학방법검측MCP-1단백적표체.결과 여대조조상비,UC장점막MCP-1 mRNA화기단백과도표체,MCP-1 mRNA표체여질병엄중정도성정상관(P<0.01).MCP-1단백표체위우UC장점막고유층단핵세포화T림파세포,병리분급Ⅲ、Ⅳ급장점막MCP-1단백표체교병리분급Ⅰ、Ⅱ급조명현증가(t=7.31,P<0.01).결론 UC점막조직중MCP-1표체수평명현상조,기표체수평여병정경중화염증엄중정도정정상관.
Objective To investigate the expression of monocyte chemoattractant protein 1(MCP-1)in the colonic mucosa of patients with ulcerative colitis(uc),and the correlation with the degree of inflammation in the co-Ionic mucosa and clinical severity of UC and the effect of MCP-1 in the pathogenesis of UC.Methods Colonic muco-sa were obtained from 32 patients with UC who underwent colonoscopy.Patients were also classified histologically as inflammation grade Ⅲ、Ⅳ(20 cases)and inflammation grade Ⅰ,Ⅱ(12 cases).Normal colonic tissues were obtained from 15 healthy adults.Semi-quantitative Reverse transcription polymerase chain reaction(RT-PCR)and immunohis-tochemistry were used to determine the expression of MCP-1.Results Both MCP-1 mRNA and protein expression lev-el were significantly enhanced in mucosa of UC compared with those of healthy control.The level of MCP-1 mRNA ex-pression Was positively correlated to the severity of UC(P<0.01).Immunohistochemisty results showed moderate to strong MCP-1 immunoreactivity which Wag localized in mononuclear cells and T lymphocytes of lamina propria in UC.Patients with inflammation grade Ⅲ,Ⅳ exhibited significantly higher expression of MCP-1 than those with inflamma-tion grade Ⅰ,Ⅱ(t=7.31,P<0.01).Conclusions MCP-1 is overexpressed in colonic mucosa in patients with UC,and the intensity of MCP-1 expression is positively correlated with the degrees of inflammation and the severity of disease.
