CAJ | 학술논문

目的:应用电镜观察二氧化钛纳米颗粒(nano-TiO_2)对小鼠单核巨噬细胞超微结构的影响.方法:将不同浓度的nano-TiO_2溶液加入小鼠单核巨噬细胞(Raw cell)中,在37℃、5%CO_2的细胞培养箱中孵育4h,收集细胞,制样后在透射电镜下观察小鼠单核巨噬细胞超微结构的改变.结果:电镜下,nano-TiO_2的粒径为20～35 nm.致密的nano-TiO_2通过内吞进入细胞,当浓度超过200μg/mL时,细胞胞质空泡内吞噬有大量成簇的致密颗粒,细胞核固缩,染色质浓缩、边集,出现早期细胞凋亡改变.
목적:응용전경관찰이양화태납미과립(nano-TiO_2)대소서단핵거서세포초미결구적영향.방법:장불동농도적nano-TiO_2용액가입소서단핵거서세포(Raw cell)중,재37℃、5%CO_2적세포배양상중부육4h,수집세포,제양후재투사전경하관찰소서단핵거서세포초미결구적개변.결과:전경하,nano-TiO_2적립경위20～35 nm.치밀적nano-TiO_2통과내탄진입세포,당농도초과200μg/mL시,세포포질공포내탄서유대량성족적치밀과립,세포핵고축,염색질농축、변집,출현조기세포조망개변.
Objective: To study the ultrastructural effect of nano-Ti02 on cultured mouse monocyte-macrophage. Method: Mouse monocyte-macrophages (Raw cell) were incubated in culture medium containing nano-TiO_2 of different concentration for 4h. The ultrastructural changes of mouse monocyte-macrophage were observed under electron microscope. Result: Under electron microscope, the diameter of nano-TiO_2 is about 20 ～ 35 nm. The dense nano-TiO_2 particles enter into the cytoplasm of mouse monocyte-macrophage by way of endocytosis. Various number of dense nano-TiO_2 particles were found in the cytoplasmic vacuoles of different size of treated macrophages . The degree of severity of cell damage increased with the increase m concentration of nano-TiO_2 in the culture medium . Apoptosis occurred in macrophages cocultured in high concentration of nano-Ti02 (over 200 μg/mL) .