CAJ | 학술논문

目的探讨7.0T小动物MRI及Micro-PET检测动脉粥样硬化的可行性.方法取10只46周龄ApoE-/-小鼠建立老年ApoE-/-小鼠动脉粥样硬化模型,取5只模型于鼠尾静脉注射超顺磁性氧化铁(SPIO)前、后12 h、24 h、36 h 行MR扫描(应用7.0TMR仪);另5只模型于鼠尾静脉注射18F-氟脱氧葡萄糖(~(18)F-FDG)1 h、2 h、3 h后行Micro-PET显像;取腹主动脉行病理检查.结果老年ApoE-/-小鼠高脂喂养6个月后,7.0T MRI证实动脉粥样硬化斑块形成;鼠尾静脉注射SPIO后36 h发现鼠腹主动脉及两侧髂动脉血管壁T2W像上环形高信号较未注射SPIO前变薄,血管腔较前增宽.鼠尾静脉注射~(18)F-FDG行Micro-PET检测,发现在3 h左右腹主动脉及两侧髂动脉区域有放射性浓聚.病理示腹主动脉壁内有动脉粥样硬化斑块形成及巨噬细胞的聚集.结论 7.0T MRI及Micro-PET可成功检测巨噬细胞丰富的动脉粥样硬化斑块,有助于判断斑块的易损性,为早期发现、诊断及治疗动脉粥样硬化提供理论依据.
목적 탐토7.0T소동물MRI급Micro-PET검측동맥죽양경화적가행성.방법 취10지46주령ApoE-/-소서건립노년ApoE-/-소서동맥죽양경화모형,취5지모형우서미정맥주사초순자성양화철(SPIO)전、후12 h、24 h、36 h 행MR소묘(응용7.0TMR의);령5지모형우서미정맥주사18F-불탈양포도당(~(18)F-FDG)1 h、2 h、3 h후행Micro-PET현상;취복주동맥행병리검사.결과 노년ApoE-/-소서고지위양6개월후,7.0T MRI증실동맥죽양경화반괴형성;서미정맥주사SPIO후36 h발현서복주동맥급량측가동맥혈관벽T2W상상배형고신호교미주사SPIO전변박,혈관강교전증관.서미정맥주사~(18)F-FDG행Micro-PET검측,발현재3 h좌우복주동맥급량측가동맥구역유방사성농취.병리시복주동맥벽내유동맥죽양경화반괴형성급거서세포적취집.결론 7.0T MRI급Micro-PET가성공검측거서세포봉부적동맥죽양경화반괴,유조우판단반괴적역손성,위조기발현、진단급치료동맥죽양경화제공이론의거.
Objective To explore the feasibility of detecting atherosclerosis with 7.0T MR and Micro-PET. Methods Ten 46-week-old ApoE-/- mice with high lipid diet for 6 months were selected to establish atherosclerosis models. Among them, 5 mice underwent MRI before and 12 h, 24 h, 36 h after injection of SPIO, respectively, and the other 5 mice were injected with ~(18)F-fluorodeoxyglucose (~(18)F-FDG) through tail vein and observed with Micro-PET after 1 h, 2 h and 3 h. The specimens of abdominal aorta were taken for pathologic examination. Results Atherosclerotic plaques were observed in all animals with 7.0T MRI after 6 months high lipid diet. Thirty-six hours after the injection of SPIO, the high signal rings were thinner and the lumen of blood vessels were wider than those before injection on T2WI. Radioactive concentration was observed in abdominal aorta and both sides of iliac artery 3 h after the injection of ~(18)F-fluorodeoxyglucose (~(18)F-FDG). Pathological examination showed the formation of atherosclerotic plaques and the aggregation of the macrophages. Conclusion 7.0T MRI and Micro-PET can be used to observe the macrophage-rich plaque and to judge the vulnerability of plaque, thus provide theoretical basis for early detection, diagnosis and treatment of atherosclerosis.