CAJ | 학술논문

呼吸与排泄是贝类新陈代谢的基本生理活动,不同盐度对贝类的生长起着至关重要的作用.本研究在28 ℃条件下,设八个盐度梯度(16、20、24、28、32、36、40、44),采用Winkle滴定法和次溴酸盐氧化法测定了大、中、小三种规格的黄边糙鸟蛤的耗氧率和排氨率.结果表明,黄边糙鸟蛤壳长与组织干重之间符合幂函数关系W = 0.004 4 H~(3.852 0),R~2 = 0.949 6;在盐度16～28范围内,黄边糙鸟蛤单位体重耗氧率随盐度的升高而增加,在盐度28取得最大值,其后随盐度上升,耗氧率呈下降趋势.方差分析(ANOVA)显示,盐度变化对黄边糙鸟蛤的耗氧率有显著影响(F=3.50, P<0.01,n=7).单位体重耗氧率和组织干重之间的关系用幂函数RO = aW~b表示,其中a值范围为0.619 8～0.956 7,b值范围为0.371 5～0.582 8.排氨率与耗氧率呈现相同的变化趋势,不同盐度下黄边糙鸟蛤的排氨率有显著差异(F=13.37, P<0.01, n=7).其排氨率和组织干重之间的关系也可用幂函数ER = aW~b表示,其中a值范围为92.94～136.65,b值范围为0.397 0～0.564 3;规格大小对黄边糙鸟蛤单位体重耗氧率和排氨率有显著影响(F=28.57, P<0.01;F=130.66, P<0.01).在盐度44条件下,驯化养殖3 d后黄边糙鸟蛤全部死亡,表明黄边糙鸟蛤的最高耐受盐度为44.
호흡여배설시패류신진대사적기본생리활동,불동염도대패류적생장기착지관중요적작용.본연구재28 ℃조건하,설팔개염도제도(16、20、24、28、32、36、40、44),채용Winkle적정법화차추산염양화법측정료대、중、소삼충규격적황변조조합적모양솔화배안솔.결과표명,황변조조합각장여조직간중지간부합멱함수관계W = 0.004 4 H~(3.852 0),R~2 = 0.949 6;재염도16～28범위내,황변조조합단위체중모양솔수염도적승고이증가,재염도28취득최대치,기후수염도상승,모양솔정하강추세.방차분석(ANOVA)현시,염도변화대황변조조합적모양솔유현저영향(F=3.50, P<0.01,n=7).단위체중모양솔화조직간중지간적관계용멱함수RO = aW~b표시,기중a치범위위0.619 8～0.956 7,b치범위위0.371 5～0.582 8.배안솔여모양솔정현상동적변화추세,불동염도하황변조조합적배안솔유현저차이(F=13.37, P<0.01, n=7).기배안솔화조직간중지간적관계야가용멱함수ER = aW~b표시,기중a치범위위92.94～136.65,b치범위위0.397 0～0.564 3;규격대소대황변조조합단위체중모양솔화배안솔유현저영향(F=28.57, P<0.01;F=130.66, P<0.01).재염도44조건하,순화양식3 d후황변조조합전부사망,표명황변조조합적최고내수염도위44.
Respiration and excretion are the basic physiological activities of bivalves and salinity also has a significant influence on the growth of bivalves. The oxygen consumption and ammonia-N excretion rate of clam Trachycardium flavum with different body sizes (L, M and S) at eight salinity gradients (16, 20, 24, 28, 32, 36, 40 and 44) (28 ℃) were studied by Winkle titration method and hypobromite oxidation method. The shell length and dry tissue weight has the power function relationship: W = 0.004 4 H~(3.852 0), R~2 = 0.949 6. The results showed that within the salinity range of 16-28, the oxygen consumption rate of T. flavum per unit dry tissue weight increased with the increasing salinity, reached the maximum at salinity 28 and then decreased with the increasing salinity. Variance analysis showed that salinity changes had significant influence on the oxygen consumption rate of T. flavum (F=3.50, P<0.01,n=7). The relationship between oxygen consumption rate and dry tissue weight could be expressed as power function equation: RO = aW~b, where a value ranged from 0.619 8 to 0.956 7, b ranged from 0.371 5 to 0.582 8. The ammonia-N excretion rate showed the same tendency with the respiration rate and significant differences were found among various salinities (F=13.37, P<0.01, n=7). The relationship between ammonia-N excretion rate and dry tissue weight could be expressed as power function equation: ER = aW~b, where a value ranged from 92.94 to 136.65, b ranged from 0.3970 to 0.5643. Body weight also had significant influences on both parameters (F=28.57, P<0.01; F=130.66, P<0.01).At salinity 44, the clams all died after three days of post-acclimation, indicating that 44 could be the maximum tolerance salinity for T. flavum.