中国抗生素杂志
中國抗生素雜誌
중국항생소잡지
CHINESE JOURNAL OF ANTIBIOTICS
2010年
4期
262-264,269
,共4页
张永红%吴军营%张志华%王立松%郄丽萍%田红
張永紅%吳軍營%張誌華%王立鬆%郄麗萍%田紅
장영홍%오군영%장지화%왕립송%극려평%전홍
雷莫拉宁%诱变%抗性选育
雷莫拉寧%誘變%抗性選育
뢰막랍저%유변%항성선육
Ramoplanin%Mutagenesis%Resistant screening
以雷莫拉宁产生菌RM05-55为出发菌株,首先采用紫外诱变复合链霉素抗性筛选,然后进行微波诱变,获得一株雷莫拉宁高产菌株W07-28,其发酵单位较出发菌株提高了59.3%.传代试验表明该菌株的发酵水平较稳定.
以雷莫拉寧產生菌RM05-55為齣髮菌株,首先採用紫外誘變複閤鏈黴素抗性篩選,然後進行微波誘變,穫得一株雷莫拉寧高產菌株W07-28,其髮酵單位較齣髮菌株提高瞭59.3%.傳代試驗錶明該菌株的髮酵水平較穩定.
이뢰막랍저산생균RM05-55위출발균주,수선채용자외유변복합련매소항성사선,연후진행미파유변,획득일주뢰막랍저고산균주W07-28,기발효단위교출발균주제고료59.3%.전대시험표명해균주적발효수평교은정.
A ramoplanin producing original strain RM05-55 was treated by UV and streptomycin-resistance,and then treated by microwave radiation.A ramoplanin high-yield strain W07-28 was obtained,and its productivity was increased by 59.3% than starting strain.Submerged culture indicated that the hereditary character of this high-yield strain was stable.
