国际呼吸杂志
國際呼吸雜誌
국제호흡잡지
INTERNATIONAL JOURNAL OF RESPIRATION
2008年
19期
1166-1170
,共5页
朱敏%王辰%张黎明%庞宝森%翟振国%杨媛华%牛淑洁%毛燕玲%马力%翁心植
硃敏%王辰%張黎明%龐寶森%翟振國%楊媛華%牛淑潔%毛燕玲%馬力%翁心植
주민%왕신%장려명%방보삼%적진국%양원화%우숙길%모연령%마력%옹심식
肺血栓栓塞症%肺损伤%基质金属蛋白酶%血管内皮生长因子
肺血栓栓塞癥%肺損傷%基質金屬蛋白酶%血管內皮生長因子
폐혈전전새증%폐손상%기질금속단백매%혈관내피생장인자
Pulmonary thromboembolism%Lung injury%Matrix metalloproteinases%Vascularendothelial growth factor
目的 通过颈外静脉注入自体血栓建立大鼠急性肺血栓栓塞症(pulmonarythromboembolism,PTE)模型,观察急性PTE后基质金属蛋白酶9(matrix metalloproteinases-9,MMP-9)和血管内皮生长因子(vascular endothelial growth factor,VEGF)的水平变化和表达,及其在PTE后肺组织病理改变中的作用.方法 56只健康雄性SD大鼠随机均分为PTE组及假手术(sham)组.于制模后2 h、6 h、24 h及72 h处死大鼠.应用苏木精-伊红染色方法观察肺组织病理改变.采用酶联免疫吸附试验法测定血清及支气管肺泡灌洗液(BALF)中MMP-9和VEGF浓度;考马斯亮兰法测定BALF中总蛋白浓度并进行细胞计数;免疫组织化学染色观察MMP-9和VEGF在肺组织中的表达.结果 ①病理结果:PTE组栓塞区肺组织有肺损伤改变;sham组未见上述病理变化;②血清学结果:PTE组MMP-9和VEGF浓度在6 h、24 h和72 h显著高于sham组(P<0.01);③BALF结果:PTE组MMP-9浓度24 h时显著高于sham组(P<0.01);VEGF 2 h和24 h均显著高于sham组(P<0.05,P<0.01);PTE组BALF中总蛋白浓度和细胞数2 h和24 h组均显著高于sham组(P值均<0.01);④免疫组织化学结果:显示PTE组MMP-9和VEGF表达显著高于sham组.结论 急性PTE可引起肺损伤.PTE可引起血液中MMP-9和VEGF细胞因子释放及其肺组织中显著表达,促进炎性细胞和蛋白渗出,参与PTE相关肺损伤的发生.
目的 通過頸外靜脈註入自體血栓建立大鼠急性肺血栓栓塞癥(pulmonarythromboembolism,PTE)模型,觀察急性PTE後基質金屬蛋白酶9(matrix metalloproteinases-9,MMP-9)和血管內皮生長因子(vascular endothelial growth factor,VEGF)的水平變化和錶達,及其在PTE後肺組織病理改變中的作用.方法 56隻健康雄性SD大鼠隨機均分為PTE組及假手術(sham)組.于製模後2 h、6 h、24 h及72 h處死大鼠.應用囌木精-伊紅染色方法觀察肺組織病理改變.採用酶聯免疫吸附試驗法測定血清及支氣管肺泡灌洗液(BALF)中MMP-9和VEGF濃度;攷馬斯亮蘭法測定BALF中總蛋白濃度併進行細胞計數;免疫組織化學染色觀察MMP-9和VEGF在肺組織中的錶達.結果 ①病理結果:PTE組栓塞區肺組織有肺損傷改變;sham組未見上述病理變化;②血清學結果:PTE組MMP-9和VEGF濃度在6 h、24 h和72 h顯著高于sham組(P<0.01);③BALF結果:PTE組MMP-9濃度24 h時顯著高于sham組(P<0.01);VEGF 2 h和24 h均顯著高于sham組(P<0.05,P<0.01);PTE組BALF中總蛋白濃度和細胞數2 h和24 h組均顯著高于sham組(P值均<0.01);④免疫組織化學結果:顯示PTE組MMP-9和VEGF錶達顯著高于sham組.結論 急性PTE可引起肺損傷.PTE可引起血液中MMP-9和VEGF細胞因子釋放及其肺組織中顯著錶達,促進炎性細胞和蛋白滲齣,參與PTE相關肺損傷的髮生.
목적 통과경외정맥주입자체혈전건립대서급성폐혈전전새증(pulmonarythromboembolism,PTE)모형,관찰급성PTE후기질금속단백매9(matrix metalloproteinases-9,MMP-9)화혈관내피생장인자(vascular endothelial growth factor,VEGF)적수평변화화표체,급기재PTE후폐조직병리개변중적작용.방법 56지건강웅성SD대서수궤균분위PTE조급가수술(sham)조.우제모후2 h、6 h、24 h급72 h처사대서.응용소목정-이홍염색방법관찰폐조직병리개변.채용매련면역흡부시험법측정혈청급지기관폐포관세액(BALF)중MMP-9화VEGF농도;고마사량란법측정BALF중총단백농도병진행세포계수;면역조직화학염색관찰MMP-9화VEGF재폐조직중적표체.결과 ①병리결과:PTE조전새구폐조직유폐손상개변;sham조미견상술병리변화;②혈청학결과:PTE조MMP-9화VEGF농도재6 h、24 h화72 h현저고우sham조(P<0.01);③BALF결과:PTE조MMP-9농도24 h시현저고우sham조(P<0.01);VEGF 2 h화24 h균현저고우sham조(P<0.05,P<0.01);PTE조BALF중총단백농도화세포수2 h화24 h조균현저고우sham조(P치균<0.01);④면역조직화학결과:현시PTE조MMP-9화VEGF표체현저고우sham조.결론 급성PTE가인기폐손상.PTE가인기혈액중MMP-9화VEGF세포인자석방급기폐조직중현저표체,촉진염성세포화단백삼출,삼여PTE상관폐손상적발생.
Objective To investigate the role of matrix metalloproteinases-9 (MMP-9) and vascularendothelial growth factor (VEGF) in the progression of lung pathological alteration associated withpulmonary thromboembolism(PTE) induced by injecting thromboemboli into external jugular veins of rats.Methods Fifty-six male Sprague Dawley rats were randomly divided into PTE group and sham group.Experimental PTE models were induced by injecting emboli into external jugular veins of rats. Other animalsreceived only saline served as shams. The rats were sacrificed at 2 h,6 h,24 h and 72 h after the models wereinduced. (1)The histopathological evaluation was performed by haematine-eosin staining. (2)Blood serum andbronchoalveolar lavage fluids(BALF) were analyzed for MMP-9 and VEGF by ELISA methods. (3)The totalprotein and cells count in BALF were examined. (4)The expressions of MMP-9 and VEGF were evaluated byimmunohistochemical staining. Results (1) Histopathological results: there were changes of lung injury inembolic zone. No same pathologic changes were seen in sham group. (2)The detecting results of bloodcytokines:the serum levels of MMP-9 and VEGF were elevated significantly in PTE group compared withthose in sham rats at 6 h, 24 h and 72 h, respectively(P<0.01). (3)The detecting results of BALF:compared with that of shams,the level of MMP-9 in BALF elevated significantly at 24 h(P<0.05),and thelevel of VEGF was significantly increased at 2 h and 24 h in PTE group(P<0.05; P<0.01 respectively).In PTE group the total protein and cells count increased significantly at 2 h and 24 h than those of shamgroups (both P<0.01). In PTE groups the serum level of MMP-9 had significant correlation with that of thetotal protein and cells count(P<0.05, P<0.05). (4)The expression of MMP-9 and VEGF were morepositively in PTE group than that of sham group in the lung parenchyma by immunohistochemical stain.Conclusions Acute PTE rat model could lead to lung injury. PTE could cause the release of MMP-9 andVEGF. The two cytokines contribute to the extravasation of inflammatory cells and protein and theprogression of lung injury associated with PTE.
