中华眼视光学与视觉科学杂志
中華眼視光學與視覺科學雜誌
중화안시광학여시각과학잡지
CHINESE JOURNAL OF OPTOMETRY OPHTHALMOLOGY AND VISUAL SCIENCE
2011年
1期
39-42
,共4页
戎慧丰%赵青%颜华%刘贵培%刘源%刘文广
戎慧豐%趙青%顏華%劉貴培%劉源%劉文廣
융혜봉%조청%안화%류귀배%류원%류문엄
神经生长因子%胶原,重组人Ⅲ型%角膜移植%神经再生
神經生長因子%膠原,重組人Ⅲ型%角膜移植%神經再生
신경생장인자%효원,중조인Ⅲ형%각막이식%신경재생
Nerve growth factor%Collagen,recombinant human type Ⅲ%Corneal transplantation%Nerve regeneration
目的 探讨负载神经生长因子(NGF)的重组人Ⅲ型胶原(RHC-Ⅲ)移植入兔眼角膜后,对角膜神经再生的影响.方法 实验研究.16只中国大耳白兔随机分为A组和B组,每组8只,右眼为手术眼.A组兔角膜前板层植入负载5 ng/mg NGF的RHC-Ⅲ,B组植入不含NGF的RHC-Ⅲ.术后2周、1个月、3个月和6个月,每组随机取1只兔眼做角膜神经氯化金染色,光镜下观察角膜神经的修复情况,并对各时间点角膜上皮及浅基质层的神经纤维进行计数.采用独立样本t检验对A、B两组间各时间点的神经纤维数量进行比较.结果 术后2周,两组即可观察到上皮及浅基质层有新生的神经纤维;术后1个月,A组浅基质层已可见到少量新生的神经纤维相互连成网状;术后3个月,两组新生神经纤维数量明显增多,均可见到网络结构以及较多侧枝形成;术后6个月,A组新生神经纤维数量明显多于B组,且结构更为完善,但两组部分神经断端没有神经再生.A组术后2周、1个月、3个月和6个月的新生神经纤维数量分别为(6.80±0.84)、(11.80±1.10)、(25.20±1.30)、(42.40±1.82)根/100倍光镜视野;B组分别为(3.00±0.71)、(5.80±0.84)、(13.20±0.84)、(26.60±1.14)根/100倍光镜视野;A组各时间点新生神经纤维数量明显高于B组,两组差异均有统计学意义(t=7.76、9.73、17.32、16.47,P均＜0.01).结论 NGF可以明显促进兔角膜板层移植术后的神经修复.
目的 探討負載神經生長因子(NGF)的重組人Ⅲ型膠原(RHC-Ⅲ)移植入兔眼角膜後,對角膜神經再生的影響.方法 實驗研究.16隻中國大耳白兔隨機分為A組和B組,每組8隻,右眼為手術眼.A組兔角膜前闆層植入負載5 ng/mg NGF的RHC-Ⅲ,B組植入不含NGF的RHC-Ⅲ.術後2週、1箇月、3箇月和6箇月,每組隨機取1隻兔眼做角膜神經氯化金染色,光鏡下觀察角膜神經的脩複情況,併對各時間點角膜上皮及淺基質層的神經纖維進行計數.採用獨立樣本t檢驗對A、B兩組間各時間點的神經纖維數量進行比較.結果 術後2週,兩組即可觀察到上皮及淺基質層有新生的神經纖維;術後1箇月,A組淺基質層已可見到少量新生的神經纖維相互連成網狀;術後3箇月,兩組新生神經纖維數量明顯增多,均可見到網絡結構以及較多側枝形成;術後6箇月,A組新生神經纖維數量明顯多于B組,且結構更為完善,但兩組部分神經斷耑沒有神經再生.A組術後2週、1箇月、3箇月和6箇月的新生神經纖維數量分彆為(6.80±0.84)、(11.80±1.10)、(25.20±1.30)、(42.40±1.82)根/100倍光鏡視野;B組分彆為(3.00±0.71)、(5.80±0.84)、(13.20±0.84)、(26.60±1.14)根/100倍光鏡視野;A組各時間點新生神經纖維數量明顯高于B組,兩組差異均有統計學意義(t=7.76、9.73、17.32、16.47,P均＜0.01).結論 NGF可以明顯促進兔角膜闆層移植術後的神經脩複.
목적 탐토부재신경생장인자(NGF)적중조인Ⅲ형효원(RHC-Ⅲ)이식입토안각막후,대각막신경재생적영향.방법 실험연구.16지중국대이백토수궤분위A조화B조,매조8지,우안위수술안.A조토각막전판층식입부재5 ng/mg NGF적RHC-Ⅲ,B조식입불함NGF적RHC-Ⅲ.술후2주、1개월、3개월화6개월,매조수궤취1지토안주각막신경록화금염색,광경하관찰각막신경적수복정황,병대각시간점각막상피급천기질층적신경섬유진행계수.채용독립양본t검험대A、B량조간각시간점적신경섬유수량진행비교.결과 술후2주,량조즉가관찰도상피급천기질층유신생적신경섬유;술후1개월,A조천기질층이가견도소량신생적신경섬유상호련성망상;술후3개월,량조신생신경섬유수량명현증다,균가견도망락결구이급교다측지형성;술후6개월,A조신생신경섬유수량명현다우B조,차결구경위완선,단량조부분신경단단몰유신경재생.A조술후2주、1개월、3개월화6개월적신생신경섬유수량분별위(6.80±0.84)、(11.80±1.10)、(25.20±1.30)、(42.40±1.82)근/100배광경시야;B조분별위(3.00±0.71)、(5.80±0.84)、(13.20±0.84)、(26.60±1.14)근/100배광경시야;A조각시간점신생신경섬유수량명현고우B조,량조차이균유통계학의의(t=7.76、9.73、17.32、16.47,P균＜0.01).결론 NGF가이명현촉진토각막판층이식술후적신경수복.
Objective To discuss the effect of recombinant human type Ⅲ collagen (RHC-Ⅲ)with nerve growth factor (NGF) on nerve regeneration after transplanting it into the rabbit cornea.Methods This was an experimental study. Sixteen rabbits were randomly divided into group A and group B, 8 rabbits in each group. The right eyes were the experimental eyes. RHC-Ⅲ with 5 ng/mg NGF and RHC-Ⅲ without NGF were implanted into the anterior lamella of the rabbit corneas in each group. One cornea was randomly selected at the 2nd week, 1st month, 3nl month and 6th month after surgery. Photochemical gold chloride impregnations were performed and the regeneration of corneal nerves was observed under light microscopy. The number of the nerve fibers in the corneal epithelium and anterior lamellar layer were calculated at all the time points. Independent samples t test was used to compare the number of fibers for the two groups. Results Nerve staining showed that nerve regeneration was already taking place in the corneal epithelium and anterior lamellar layer in both groups at the 2nd week. At the 1st month, some regenerated nerve fibers in the superficial stroma had formed a network. At the 3rd month, the number of regenerated nerve fibers had noticeably increased in both groups and more networks and lateral branches had formed.Six months later, there were a greater number of regenerated nerve fibers in group A compared to group B, and the structures in group A were more normal. However, some nerve fibers did not regenerate in either group. The numbers of regenerated nerve fibers at the four time points were (6.80±0.84), (11.80±1.10), (25.20±1.30), (42.40±1.82) per 100 times the microscopic field in group A; and (3.00±0.71), (5.80±0.84), (13.20±0.84), (26.60±1.14) per 100 times the microscopic field in group B. There were significant differences in the number of nerve fibers between group A and group B at all the observation points (t=7.76, 9.73, 17.32, 16.47, P＜0.01 for all).Conclusion NGF can improve nerve regeneration in the rabbit cornea after lamellar keratoplasty.
