CAJ | 학술논문

目的探讨上皮型钙黏附蛋白(E-cad)、β-连环素在原发性干燥综合征(pSS)的发病过程中的作用.方法应用免疫组织化学法对52例pSS患者和30名健康对照者唇腺组织E-cad、β-连环素检测,电镜观察超微结构的改变.采用Marmwhitney U检验和Spearman相关性分析.结果 ①E-cad在pSS组的表达面积[(2513±1086) μm2],吸光度(0.212±0.041)、累积吸光度(566±297)低于健康对照组(P＜0.01),随淋巴细胞浸润灶数的增加,表达呈递减趋势.②β-连环素在pSS组的表达面积[(12 324±7883)μm2],吸光度(0.113±0.031)、累积吸光度(1666±1361)低于健康对照组(P＜0.01),随淋巴细胞浸润灶数的增加,表达呈递减趋势.③E-cad与β-连环素在pSS组唇腺组织的表达呈正相关(P＜0.01).④超微结构:细胞连接疏松.⑤E-cad、β-连环索在抗SSA/SSB抗体(+)与(-)者表达差异有统计学意义(P＜0.05,P＜0.01).结论 ①E-cad、β-连环素参与了pSS的发病过程.②抗SSMSSB抗体在pSS发病过程中具有一定的作用.
목적 탐토상피형개점부단백(E-cad)、β-련배소재원발성간조종합정(pSS)적발병과정중적작용.방법 응용면역조직화학법대52례pSS환자화30명건강대조자진선조직E-cad、β-련배소검측,전경관찰초미결구적개변.채용Marmwhitney U검험화Spearman상관성분석.결과 ①E-cad재pSS조적표체면적[(2513±1086) μm2],흡광도(0.212±0.041)、루적흡광도(566±297)저우건강대조조(P＜0.01),수림파세포침윤조수적증가,표체정체감추세.②β-련배소재pSS조적표체면적[(12 324±7883)μm2],흡광도(0.113±0.031)、루적흡광도(1666±1361)저우건강대조조(P＜0.01),수림파세포침윤조수적증가,표체정체감추세.③E-cad여β-련배소재pSS조진선조직적표체정정상관(P＜0.01).④초미결구:세포련접소송.⑤E-cad、β-련배색재항SSA/SSB항체(+)여(-)자표체차이유통계학의의(P＜0.05,P＜0.01).결론 ①E-cad、β-련배소삼여료pSS적발병과정.②항SSMSSB항체재pSS발병과정중구유일정적작용.
Objective To study the expression of E-cadherin( E-cad), p-catenin(β-cat) in labial salivary glands of patients with primary Sj(o)gren's syndrome (pSS) in order to explore their role in pathogenesis. Methods Biopsies of labial salivary glands were obtained from 52 patients with pSS and 30 healthy controls. The immunohistochemical staining was used to detect the expression of E-cad and β-cat. Anti-SSA and anti-SSB antibodies were measured. Semi-quantitative analysis was performed by image analysis software. Ultra-structural changes was used by electron-microscopic techniques. Results ① The area of expression, optical intensity and the accumulated optical intensity of the E-cad group [(2513±1086) μm2, 0.212±0.041, 566 ±297 ] were lower than normal controls. The expression level was reduced as the increase of lymphocyte infiltration focus. ② The area of expression, the optical density and the accumulated optical density of the β- cat group [(12 324±7883) μm2, 0.113±0.031, 566±297] was lower than those of the control group. The expression level was reduced as the increase of the lymphocyte infiltration focus. ③ The E-cad expression and the p-cat expression was positively correlated in the labial gland of patients with pSS. ④ Howev-er, there was difference in the expression of E -cad and β -cat between patients with positive SSA and negative SSA antibodies. Conclusion In salivary samples, the expression of both E-cad and p-cat in patients with pSS is lower than those of the controls. Anti-SSA/SSB antibodies are important parameters of pSS and they may be involved in the pathogenesis of pSS.