眼科研究
眼科研究
안과연구
CHINESE OPHTHALMIC RESEARCH
2009年
12期
1073-1076
,共4页
晶状体上皮细胞%后发性白内障%二氧化钛%光催化剂膜
晶狀體上皮細胞%後髮性白內障%二氧化鈦%光催化劑膜
정상체상피세포%후발성백내장%이양화태%광최화제막
lens epithelium cell%after cataract%titanium dioxide%photocatalyst film
目的 观察经光激发后TiO_2纳米光催化剂薄膜对体外培养的牛晶状体上皮细胞(LECs)黏附及移行的影响.方法 采用溶胶-凝胶法在载玻片表面制备TiO_2光催化剂薄膜,对载玻片行接触角测量.体外培养牛LECs爬片生长于此薄膜上.未经TiO_2纳米光催化剂薄膜修饰的载玻片设为对照.进行细胞黏附和迁移实验,测定并比较牛LECs在TiO_2薄膜修饰载玻片与对照载玻片表面的生长、移行情况.结果 对TiO_2薄膜组和对照组载玻片行接触角测量:对照组载玻片接触角为18.825°±2.342°,TiO_2镀膜组载玻片接触角为0°±2°,TiO_2镀膜组载玻片的接触角明显小于对照组.通过细胞黏附实验,TiO_2镀膜组载玻片表面细胞黏附数平均为36.60±11.20,对照组细胞黏附数平均为96.40±19.67,差异有统计学意义(P=0.000).细胞迁移实验(划痕法)中,对照组细胞移行距离平均为0.951 mm,TiO_2镀膜组细胞平均移行距离为0.640 mm,差异有统计学意义(P=0.000).结论 TiO_2纳米光催化剂薄膜具有超亲水性,能够明显抑制牛LECs在其表面的黏附和移行.
目的 觀察經光激髮後TiO_2納米光催化劑薄膜對體外培養的牛晶狀體上皮細胞(LECs)黏附及移行的影響.方法 採用溶膠-凝膠法在載玻片錶麵製備TiO_2光催化劑薄膜,對載玻片行接觸角測量.體外培養牛LECs爬片生長于此薄膜上.未經TiO_2納米光催化劑薄膜脩飾的載玻片設為對照.進行細胞黏附和遷移實驗,測定併比較牛LECs在TiO_2薄膜脩飾載玻片與對照載玻片錶麵的生長、移行情況.結果 對TiO_2薄膜組和對照組載玻片行接觸角測量:對照組載玻片接觸角為18.825°±2.342°,TiO_2鍍膜組載玻片接觸角為0°±2°,TiO_2鍍膜組載玻片的接觸角明顯小于對照組.通過細胞黏附實驗,TiO_2鍍膜組載玻片錶麵細胞黏附數平均為36.60±11.20,對照組細胞黏附數平均為96.40±19.67,差異有統計學意義(P=0.000).細胞遷移實驗(劃痕法)中,對照組細胞移行距離平均為0.951 mm,TiO_2鍍膜組細胞平均移行距離為0.640 mm,差異有統計學意義(P=0.000).結論 TiO_2納米光催化劑薄膜具有超親水性,能夠明顯抑製牛LECs在其錶麵的黏附和移行.
목적 관찰경광격발후TiO_2납미광최화제박막대체외배양적우정상체상피세포(LECs)점부급이행적영향.방법 채용용효-응효법재재파편표면제비TiO_2광최화제박막,대재파편행접촉각측량.체외배양우LECs파편생장우차박막상.미경TiO_2납미광최화제박막수식적재파편설위대조.진행세포점부화천이실험,측정병비교우LECs재TiO_2박막수식재파편여대조재파편표면적생장、이행정황.결과 대TiO_2박막조화대조조재파편행접촉각측량:대조조재파편접촉각위18.825°±2.342°,TiO_2도막조재파편접촉각위0°±2°,TiO_2도막조재파편적접촉각명현소우대조조.통과세포점부실험,TiO_2도막조재파편표면세포점부수평균위36.60±11.20,대조조세포점부수평균위96.40±19.67,차이유통계학의의(P=0.000).세포천이실험(화흔법)중,대조조세포이행거리평균위0.951 mm,TiO_2도막조세포평균이행거리위0.640 mm,차이유통계학의의(P=0.000).결론 TiO_2납미광최화제박막구유초친수성,능구명현억제우LECs재기표면적점부화이행.
Objective The generate of posterior capsular opacification(PCO)is associated with the adhensive and migration of residual subcapsular lens epithelial cells(LECs).Titanium dioxide(TiO_2)nanometer is proved to have the ability of killing tumor cells and cultured bovine LECs.This study tried to observe the effects of TiO_2 nanometer thin film provoked by light on adhesiveness and migration of bovine LECs in vitro.MethodsThe fresh bovine lenses were obtained and cultured in DMEM containing 10% of newborn bovine serum.The second to fifth generation of cells were used in this experiment.The slide modified by TiO_2 photocatalyst film was prepared by sol-gel method.Cultured cells were seeded in filmed or unfilmed slides respectively and exposed to ultraviolet(wavelength 365 nm)for 20 or 40 minutes.The contact angle between water drop and slide was measured by droping method and the cells adhered to slides were calculated after 24 and 48 hours of culture.The growth status and migration distance of bovine LECs were assayed and compared between filmed and unfilmed groups.ResultsThe contact angle between water drop and slide was 0°±2°and 18.825°±2.342° in filmed and unfilmed group respectively,indicating a obviously smaller contact angle in TiO_2 filmed group than unfilmed one.The numbers of bovine LECs adhered to filmed slide was considerably reduced in TiO_2 filmed group compared with unfilmed group in different UVA exposure time(t_(0 min)=5.492,P=0.001;t_(20 min)=6.031,P=0.000;t_(40 min)=6.828,P=0.000).However,no significant difference was found in the numbers of adhensive cells among 3 UVA irradiation time points(F=1.278,P=0.297).The migration distance of the cells was significantly shorter in TiO_2 filmed group in comparison with unfilmed group in 24 and 48 hours after UVA irradiation(F_(group)=14.965,P=0.000;F_(time)=38.033,P=0.000).ConclusionThe TiO_2 nanometer thin film is characterized by the superhydrophilic property.So it can effectively impede the adhesion and migration of bovine LECs in vitro.
