中华劳动卫生职业病杂志
中華勞動衛生職業病雜誌
중화노동위생직업병잡지
CHINESE JOURNAL OF INDUSTRIAL HYGIENE AND OCCUPATIONAL DISEASES
2012年
6期
428-431
,共4页
王剑波%索炜%战锐%冷雪%闫海波%姚林%钱令嘉
王劍波%索煒%戰銳%冷雪%閆海波%姚林%錢令嘉
왕검파%색위%전예%랭설%염해파%요림%전령가
矽肺%肺纤维化%热休克蛋白质类70
矽肺%肺纖維化%熱休剋蛋白質類70
석폐%폐섬유화%열휴극단백질류70
Silicosis%Pulmonary fibrosis%Heat shock proteins 70
目的 探讨热休克蛋白70( heat shock protein 70,Hsp70)在二氧化硅诱导的大鼠肺纤维化过程中的变化.方法 48只Wistar大鼠随机分成对照组和染尘组,每组24只,分别观察7、14、21、28d.染尘组经气管内灌注二氧化硅(50mg/ml),对照组予等剂量的生理盐水.HE染色观察病理学变化,蛋白免疫印迹( Western bolt)法和免疫组织化学法检测肺组织中Hsp70的表达与定位,双抗夹心ELISA检测血浆中Hsp70含量的变化.结果 染尘组染尘后7d肺组织中Hsp70含量升高,14d达到高峰,随后降低,但仍高于正常对照.染尘组14、21、28 d时血浆中Hsp70含量明显高于生理盐水对照组,差异有统计学意义(P＜0.05).染尘组血浆中Hsp70含量21d达到峰值[(0.216±0.027) μg/ml].结论 在染尘组大鼠肺组织和血浆中Hsp70含量均明显升高,并随肺纤维化进程出现先升高后降低的趋势,提示肺组织中Hsp70在二氧化硅诱导的肺纤维化过程中有重要的生物学作用.
目的 探討熱休剋蛋白70( heat shock protein 70,Hsp70)在二氧化硅誘導的大鼠肺纖維化過程中的變化.方法 48隻Wistar大鼠隨機分成對照組和染塵組,每組24隻,分彆觀察7、14、21、28d.染塵組經氣管內灌註二氧化硅(50mg/ml),對照組予等劑量的生理鹽水.HE染色觀察病理學變化,蛋白免疫印跡( Western bolt)法和免疫組織化學法檢測肺組織中Hsp70的錶達與定位,雙抗夾心ELISA檢測血漿中Hsp70含量的變化.結果 染塵組染塵後7d肺組織中Hsp70含量升高,14d達到高峰,隨後降低,但仍高于正常對照.染塵組14、21、28 d時血漿中Hsp70含量明顯高于生理鹽水對照組,差異有統計學意義(P＜0.05).染塵組血漿中Hsp70含量21d達到峰值[(0.216±0.027) μg/ml].結論 在染塵組大鼠肺組織和血漿中Hsp70含量均明顯升高,併隨肺纖維化進程齣現先升高後降低的趨勢,提示肺組織中Hsp70在二氧化硅誘導的肺纖維化過程中有重要的生物學作用.
목적 탐토열휴극단백70( heat shock protein 70,Hsp70)재이양화규유도적대서폐섬유화과정중적변화.방법 48지Wistar대서수궤분성대조조화염진조,매조24지,분별관찰7、14、21、28d.염진조경기관내관주이양화규(50mg/ml),대조조여등제량적생리염수.HE염색관찰병이학변화,단백면역인적( Western bolt)법화면역조직화학법검측폐조직중Hsp70적표체여정위,쌍항협심ELISA검측혈장중Hsp70함량적변화.결과 염진조염진후7d폐조직중Hsp70함량승고,14d체도고봉,수후강저,단잉고우정상대조.염진조14、21、28 d시혈장중Hsp70함량명현고우생리염수대조조,차이유통계학의의(P＜0.05).염진조혈장중Hsp70함량21d체도봉치[(0.216±0.027) μg/ml].결론 재염진조대서폐조직화혈장중Hsp70함량균명현승고,병수폐섬유화진정출현선승고후강저적추세,제시폐조직중Hsp70재이양화규유도적폐섬유화과정중유중요적생물학작용.
Objective To investigate the dynamic expression of Heat shock protein 70 (Hsp70) in the lungs and plasma of rats with pulmonary fibrosis induced by silicon dioxide (SiO2).Methods Forty-eight Wistar rats were randomly divided into the control group exposed to normal solution and group exposed to SiO2 (50 mg/ml) with intratracheal injection.Each group was divided into four subgroups.The animals of SiO2 group and control group were sacrificed and lungs were collected on the 7th,14th and 28th days after exposure,respectively.The left lung tissues were examined with the histopathologic HE s taining.The expression and localization of Hsp70 protein in the lung tissues were examined with western blot assay and immunohistochemistry,respectively.The expression levels of Hsp70 protein in the plasma were measured by ELISA.Results The expression of Hsp70 in lung tissues of SiO2 group increased on the 7th day and reached the peak value on the 14th day then decreased,but still was significantly higher than that of the control group,the expression of Hsp70 in plasma of SiO2 group still was significantly higher than that of the control group (P＜0.05).The maximum expression level of Hsp70 in plasma of SiO2 group on the 21st day after exposure was 0.216 ± 0.027 μg/ml.Conclusion The expression levels of Hsp70 protein in the lung tissues and plasma of the group exposed to SiO2 significantly increased,which were associated with the process of pulmonary fibrosis.It was suggested that Hsp70 protein may play an important biological role in the pulmonary fibrosis induced by SiO2.
