中华耳鼻咽喉头颈外科杂志
中華耳鼻嚥喉頭頸外科雜誌
중화이비인후두경외과잡지
CHINESE JOURNAL OF OTORHINOLARYNGOLOGY HEAD AND NECK SURGERY
2009年
2期
145-149
,共5页
于栋祯%丁大连%殷善开%Richard J Salvi
于棟禎%丁大連%慇善開%Richard J Salvi
우동정%정대련%은선개%Richard J Salvi
毛细胞,外%耳声发射,自发性%顺铂%依他尼酸%毛丝鼠属
毛細胞,外%耳聲髮射,自髮性%順鉑%依他尼痠%毛絲鼠屬
모세포,외%이성발사,자발성%순박%의타니산%모사서속
Hair cell,outer%Otoacoustic emission,spontaneous%Cisplatin%Ethacrynic acid%Chinchilla
目的 探讨灰鼠畸变产物耳声发射(DPOAE)幅值的降低与外毛细胞缺失率之间的关系.方法 联合应用顺铂和利尿酸钠,建立灰鼠耳蜗毛细胞损伤模型.12只灰鼠在静脉注射利尿酸钠(40 mg/kg)同时,腹腔注射顺铂(0.2 mg/kg),分别于用药前和用药后的1周、2周及3周在清醒状态下检测动物DPOAE,并在施行最后1次DPOAE检测后处死动物.常规制备灰鼠耳蜗基底膜铺片并进行全耳蜗毛细胞计数.计算用药前和处死前DPOAE幅值的改变程度,通过Pearson相关分析对DPOAE幅值的降低与外毛细胞缺失程度之间的相关性进行分析.结果 顺铂和利尿酸钠引起灰鼠耳蜗外毛细胞缺失的特点是病变始发于基底膜的底回并逐渐向顶回发展,外毛细胞的缺损程度沿着基底膜从底回向顶回逐渐减轻,而不同频率DPOAE幅值降低的程度亦遵循从高频向低频发展的规律,各频率DPOAE在注射顺铂和利尿酸钠后的幅值改变与基底膜上相应部位的外毛细胞缺损程度存在一定的对应关系(r=0.796,P<0.05).外毛细胞每损失1%,可引起约0.24 dB的DPOAE幅值降低.结论 在灰鼠耳蜗损伤模型中,从DPOAE幅值的变化可以大致评估耳蜗外毛细胞的缺失程度.
目的 探討灰鼠畸變產物耳聲髮射(DPOAE)幅值的降低與外毛細胞缺失率之間的關繫.方法 聯閤應用順鉑和利尿痠鈉,建立灰鼠耳蝸毛細胞損傷模型.12隻灰鼠在靜脈註射利尿痠鈉(40 mg/kg)同時,腹腔註射順鉑(0.2 mg/kg),分彆于用藥前和用藥後的1週、2週及3週在清醒狀態下檢測動物DPOAE,併在施行最後1次DPOAE檢測後處死動物.常規製備灰鼠耳蝸基底膜鋪片併進行全耳蝸毛細胞計數.計算用藥前和處死前DPOAE幅值的改變程度,通過Pearson相關分析對DPOAE幅值的降低與外毛細胞缺失程度之間的相關性進行分析.結果 順鉑和利尿痠鈉引起灰鼠耳蝸外毛細胞缺失的特點是病變始髮于基底膜的底迴併逐漸嚮頂迴髮展,外毛細胞的缺損程度沿著基底膜從底迴嚮頂迴逐漸減輕,而不同頻率DPOAE幅值降低的程度亦遵循從高頻嚮低頻髮展的規律,各頻率DPOAE在註射順鉑和利尿痠鈉後的幅值改變與基底膜上相應部位的外毛細胞缺損程度存在一定的對應關繫(r=0.796,P<0.05).外毛細胞每損失1%,可引起約0.24 dB的DPOAE幅值降低.結論 在灰鼠耳蝸損傷模型中,從DPOAE幅值的變化可以大緻評估耳蝸外毛細胞的缺失程度.
목적 탐토회서기변산물이성발사(DPOAE)폭치적강저여외모세포결실솔지간적관계.방법 연합응용순박화이뇨산납,건립회서이와모세포손상모형.12지회서재정맥주사이뇨산납(40 mg/kg)동시,복강주사순박(0.2 mg/kg),분별우용약전화용약후적1주、2주급3주재청성상태하검측동물DPOAE,병재시행최후1차DPOAE검측후처사동물.상규제비회서이와기저막포편병진행전이와모세포계수.계산용약전화처사전DPOAE폭치적개변정도,통과Pearson상관분석대DPOAE폭치적강저여외모세포결실정도지간적상관성진행분석.결과 순박화이뇨산납인기회서이와외모세포결실적특점시병변시발우기저막적저회병축점향정회발전,외모세포적결손정도연착기저막종저회향정회축점감경,이불동빈솔DPOAE폭치강저적정도역준순종고빈향저빈발전적규률,각빈솔DPOAE재주사순박화이뇨산납후적폭치개변여기저막상상응부위적외모세포결손정도존재일정적대응관계(r=0.796,P<0.05).외모세포매손실1%,가인기약0.24 dB적DPOAE폭치강저.결론 재회서이와손상모형중,종DPOAE폭치적변화가이대치평고이와외모세포적결실정도.
Objective To explore the quantitative relationship between the reduction of distortion product otoacoustic emission (DPOAE) and the percentage of outer hair cell loss. Methods Coadministration of cisplatin (0.2 mg/kg) and ethacrynic acid (40 mg/kg) were used to estabhsh a cochlear lesion model in chinchillas. DPOAE was measured before and 1 week, 2 weeks, and 3 weeks later respectively after cisplatin and ethacrynic acid treatment. Animals were terminated 3 weeks after the treatment. Cochlear surface preparations were performed, and the cochlear hair cells were counted through entire length of the cochlea. The correlation between DPOAE reduction and outer hair cell missing was analyzed using Pearson correlation analysis. Results Cisplatin and ethacrynic acid treatment induced cochlear hair cell lesion that the outer hair cell loss in the cochlea developed in a stereotypic pattern;damage began in the base of the cochlea and progressed towards the apex. Reduction of DPOAE was relatively consistent with outer hair cells loss. On the average, 1% outer hair cells loss may result in 0.24 dB reduction in DPOAE levels. Pearson analysis showed a positive correlation between the reduction in DPOAE and missing of outer hair cells (r=0.796, P<0.05). Condusions It may be helpful to evaluate missing percentage of outer hair cells from reduction in DPOAE levels.
