中华全科医师杂志
中華全科醫師雜誌
중화전과의사잡지
CHINESE JOURNAL OF GENERAL PRACTITIONERS
2009年
4期
238-240
,共3页
虞斌%许培箴%王秋伟%董一善%张玢
虞斌%許培箴%王鞦偉%董一善%張玢
우빈%허배잠%왕추위%동일선%장분
乳头状瘤病毒感染%DNA,病毒%核酸杂交%宫颈肿瘤
乳頭狀瘤病毒感染%DNA,病毒%覈痠雜交%宮頸腫瘤
유두상류병독감염%DNA,병독%핵산잡교%궁경종류
Papillomavirus infections%DNA,viral%Nucleic acid hybridization%Uterine cervical neoplasms
目的 探讨高危型人乳头状瘤病毒(HPV)DNA与宫颈痛生物学行为的相关性.方法 对66例宫颈癌患者及103例宫颈癌行手术治疗后随访病例宫颈脱落细胞,采用第二代杂交捕获技术行高危型HPV DNA检测.结果 ①66例中62例HPV DNA检测阳性,阳性率达94%,仅4例阴性(6%).②官颈癌患者各临床分期、病理分级间HPV DNA阳性率差异无统计学意义,但发生宫颈间质侵入的宫颈癌患者HPV阳性率及病毒DNA载载均明显上升,差异有统计学意义(P<0.05).③96%(99/103)的宫颈痛经手术治疗后患者HPV DNA检测为阴性.结论 高危型HPV感染可能与宫颈癌血管生成、浸润和转移有关;第二代杂交捕获技术为高效的宫颈癌筛查方法.
目的 探討高危型人乳頭狀瘤病毒(HPV)DNA與宮頸痛生物學行為的相關性.方法 對66例宮頸癌患者及103例宮頸癌行手術治療後隨訪病例宮頸脫落細胞,採用第二代雜交捕穫技術行高危型HPV DNA檢測.結果 ①66例中62例HPV DNA檢測暘性,暘性率達94%,僅4例陰性(6%).②官頸癌患者各臨床分期、病理分級間HPV DNA暘性率差異無統計學意義,但髮生宮頸間質侵入的宮頸癌患者HPV暘性率及病毒DNA載載均明顯上升,差異有統計學意義(P<0.05).③96%(99/103)的宮頸痛經手術治療後患者HPV DNA檢測為陰性.結論 高危型HPV感染可能與宮頸癌血管生成、浸潤和轉移有關;第二代雜交捕穫技術為高效的宮頸癌篩查方法.
목적 탐토고위형인유두상류병독(HPV)DNA여궁경통생물학행위적상관성.방법 대66례궁경암환자급103례궁경암행수술치료후수방병례궁경탈락세포,채용제이대잡교포획기술행고위형HPV DNA검측.결과 ①66례중62례HPV DNA검측양성,양성솔체94%,부4례음성(6%).②관경암환자각림상분기、병리분급간HPV DNA양성솔차이무통계학의의,단발생궁경간질침입적궁경암환자HPV양성솔급병독DNA재재균명현상승,차이유통계학의의(P<0.05).③96%(99/103)적궁경통경수술치료후환자HPV DNA검측위음성.결론 고위형HPV감염가능여궁경암혈관생성、침윤화전이유관;제이대잡교포획기술위고효적궁경암사사방법.
Objective To explore the relationship between high-risk human papillomavirus (HPV) DNA and biological behavior of cervical carcinoma. Methods Sixty-six patients of cervical carcinoma with cytological examinations and 103 patients of cervical carcinoma followed-up after surgical operation were selected for high-risk HPV DNA test with second-generation hybrid capture technique (HC2 Ⅱ). Results ①HPV DNA was positive in 62 and negative in four of 66 patients of cervical carcinoma with an overall prevalence of 94%. ②There was no significant difference in positive HPV DNA of patients with cervical carcinoma between their varied clinical stages and pathologic grades. But, HPV positivity and HPV DNA load in patients with myometrial invasion were higher than those in patients without invasion (P < 0. 05).③ HPV DNA conversed to negative in 99 of 103 patients (96%) with cervical carcinoma after surgical operation from positivity before operation. Conclusions High-risk HPV infection may correlate with angiogenesis, invasion and metastasis of cervical carcinoma and HC2 Ⅱ can be used as an effective method to detect HPV DNA.