CAJ | 학술논문

目的了解深圳市宿主动物携带汉坦病毒状况、病毒型别及分子特征.方法笼日法捕鼠,计算鼠密度,确定鼠种构成.共捕获宿主动物472只,褐家鼠为优势鼠种,占87.29%.免疫荧光抗原阳性鼠肺标本中提取病毒RNA,应用汉坦病毒型特异性引物进行逆转录.套式PCR扩增M片段基因和核苷酸序列测定,对汉坦病毒进行基因分型和同源性分析.结果 21只来自我市不同区褐家鼠的鼠肺标本中均发现携带汉城型病毒,汉坦病毒M片段核苷酸同源性高达95.4%以上,属于同一亚型.系统进化树显示它们可以分为6个小进化分支,与SZ2083进化关系相对最近.但发现来源于地理位置较近的病毒株并没有显示出较高的基因组核苷酸序列的同源性,反而相隔较远的病毒株呈现出一致性.结论深圳市汉坦病毒的主要宿主动物是褐家鼠,携带的汉城型汉坦病毒之间的核苷酸序列及其推导的氨基酸序列同源性较高,但也存在一定的差异.这种差异形成的原因有待进一步研究.
목적 료해심수시숙주동물휴대한탄병독상황、병독형별급분자특정.방법 롱일법포서,계산서밀도,학정서충구성.공포획숙주동물472지,갈가서위우세서충,점87.29%.면역형광항원양성서폐표본중제취병독RNA,응용한탄병독형특이성인물진행역전록.투식PCR확증M편단기인화핵감산서렬측정,대한탄병독진행기인분형화동원성분석.결과 21지래자아시불동구갈가서적서폐표본중균발현휴대한성형병독,한탄병독M편단핵감산동원성고체95.4%이상,속우동일아형.계통진화수현시타문가이분위6개소진화분지,여SZ2083진화관계상대최근.단발현래원우지리위치교근적병독주병몰유현시출교고적기인조핵감산서렬적동원성,반이상격교원적병독주정현출일치성.결론 심수시한탄병독적주요숙주동물시갈가서,휴대적한성형한탄병독지간적핵감산서렬급기추도적안기산서렬동원성교고,단야존재일정적차이.저충차이형성적원인유대진일보연구.
Objective In order to investigate Hantavirus (HV) infection of captured rodents and to understand the genotypes and the molecular characteristic of Hantaviruses in Shenzhen. Methods The captured rodents were classified and the density of distribution was calculated. A total of 472 animals were captured, among which Rattus norvegicus was the dominant group. The total viral RNA was extracted from the lung tissues positive with HV antigens by immunofluorescent assay and gene sequence of M fragment was amplified with RT-nested-PCR by using the Hantavirus genotype specific primers. The amplified genes were then sequenced, and subjected to genotyping and homology analysis. Results The results of genotype analysis showed that the Hantaviruses taken from twenty-one lung specimens in Rattus norvegicus in Shenzhen city belonged to the Hantavirus type Ⅱ (SEOV). Results in homology analysis suggested that the homology among twenty-one samples should be rather high with 95.4% of nucleotide sequence identity and they belonged to the same subtype. Phylogenetic tree analysis showed that they were branched into at least six different lineages, and were highly homologized with SZ2083. We also found that these virus strains had not shown more highly homology of nucleotide sequence in nearest district, whereas revealed consistency in farther district. Conclusion The major hosts of Hantaviruses in Shenzhen city were Rattus norvegicus and the epidemic strains were genotyped as SEO-type. Nucleotide sequence and deduced amino acid sequence from different rodents were highly homologous, while nucleotide mutation had also been observed. Further studies are required to explore the possible viruses' sequence mutation.