CAJ | 학술논문

[目的]鉴定免疫鸡群肾型传染性支气管炎病毒(IBV).[方法]以接种IBV的鸡胚尿囊液为模板,根据GenBank中登录的IBV的N基因序列设计引物,通过RT-PCR扩增出目的片段,经酶切鉴定后对其进行测序鉴定.[结果]PCR扩增片段长度为409 bp,将其N基因核苷酸序列与不同地区分离株(山东、黑龙江等)以及欧洲呼吸型疫苗株(M41)的IBV核苷酸序列进行比较,结果表明,不同毒株的核苷酸同源性在85.6%～100.0%,此次分离所得IBV的N基因与IBV SD0708株(EU352625)N基因的核苷酸同源性高达99.3%,而与呼吸型疫苗株M41(M28566)的同源性仅为87.3%.测序结果及序列分析可以证实分离到的病毒为IBV,命名为HN/HL株.[结论]该研究为鸡传染性支气管炎病毒的控制奠定了基础.
[목적]감정면역계군신형전염성지기관염병독(IBV).[방법]이접충IBV적계배뇨낭액위모판,근거GenBank중등록적IBV적N기인서렬설계인물,통과RT-PCR확증출목적편단,경매절감정후대기진행측서감정.[결과]PCR확증편단장도위409 bp,장기N기인핵감산서렬여불동지구분리주(산동、흑룡강등)이급구주호흡형역묘주(M41)적IBV핵감산서렬진행비교,결과표명,불동독주적핵감산동원성재85.6%～100.0%,차차분리소득IBV적N기인여IBV SD0708주(EU352625)N기인적핵감산동원성고체99.3%,이여호흡형역묘주M41(M28566)적동원성부위87.3%.측서결과급서렬분석가이증실분리도적병독위IBV,명명위HN/HL주.[결론]해연구위계전염성지기관염병독적공제전정료기출.
[Objective] The aim was to identify kidney-type infectious bronchitis virus (IBV) from immunized chicken.[Method] The target fragment was amplified from the allantoic fluid mRNA of IBV by RT-PCR using the primers designed from N gene sequence of IBV entered in GenBank and after identified with enzyme restriction, it was sequenced.[Result] The length of the amplified fragment was 409bp.Compared its N gene nucleotide acid sequence with IBV gene nucleotide acid sequence from isolated strains in different areas such as Shandong and Heilongjiang and European respiratory vaccine strain M41, the result showed that the nucleotide acid homology among different strains was between 85.6% and 100.0%.The N gene nucleotide acid homology between the separated IBV and IBV SD0708 strain was up to 99.3%, and that between the separated IBV and respiratory vaccine strain M41 was only 87.3%.The sequencing result and sequence analysis could confirm the isolated virus was IBV, which was named HN/HL strain.[Conclusion] The research laid the foundation for the control of chicken infectious bronchitis virus.