CAJ | 학술논문

目的介绍一种诱导神经干细胞向神经元定向分化的方法 . 方法采用选择性无血清培养液培养大鼠神经干细胞.纯化培养2或3代后,将其接种于条件培养液中,诱导其向神经元定向分化.用倒置显微镜观察形态学变化,用NSE免疫细胞化学法检测其向神经元分化情况. 结果条件培养液可有效诱导神经干细胞向神经元定向分化.将神经球消化成单细胞后接种,不但分化过程比神经球接种更同步,而且可提高其向神经元分化的比例. 结论本方法可稳定高效地诱导神经干细胞向神经元定向分化.
목적 개소일충유도신경간세포향신경원정향분화적방법 . 방법 채용선택성무혈청배양액배양대서신경간세포.순화배양2혹3대후,장기접충우조건배양액중,유도기향신경원정향분화.용도치현미경관찰형태학변화,용NSE면역세포화학법검측기향신경원분화정황. 결과 조건배양액가유효유도신경간세포향신경원정향분화.장신경구소화성단세포후접충,불단분화과정비신경구접충경동보,이차가제고기향신경원분화적비례. 결론 본방법 가은정고효지유도신경간세포향신경원정향분화.
Objective To introduce a reliable method to induce neural stem cells directed differentiating into neurons in vitro. Methods The rat neural stem cells were cultured in selected serum-free medium. After cultured for 2, 3 passages,the neurospheres or single-cells isolated from neurospheres were cultured in conditioned medium to induce directed differentiating into neurons. Besides morphological observation of those cells under inverted microscope, NSE immunocytochemistry was carried out to detect the differentiating ratio of those cells from neural stem cells into neurons. Results It was shown that the conditioned medium could induce neural stem cells differentiating into neurons effectively in both neurospheres culture mode or single-cells culture mode. Furthermore, compared with the neurospheres culture mode, the single-cells culture mode showed more synchronous in the differentiation process and higher in the percentage of NSE-positive cells. Conclusion This method can induce neural stem cells directed differentiating into neurons effectively and stablely.