CAJ | 학술논문

目的:探寻栀子中具有改善睡眠作用的活性成分.方法:健康ICR小鼠,分设栀子苷低剂量组(65mg/kg bw)、栀子苷中剂量组(650mg/kg bw)、栀子苷高剂最组(1950mg/kg bw),栀了黄色素低剂量组(30mg/kg bw)、栀子黄色素中剂量组(300mg/kg bw)、栀子黄色素高剂量组(900mg/kg bw)和空白对照组(蒸馏水),连续灌胃28d后,测定各项睡眠指标.结果:与对照组比较,栀子黄色素低、中、高剂量组分别将小鼠睡眠潜伏期缩短了18.2%(P<0.05)、21.4%(P<0.01)、26.2%(P<0.01);同时,将小鼠睡眠时间分别延长了32.4%(P<0.05)、48.1%(P<0.01)、52.4%(P<0.01).栀子苷对睡眠无明显的改善作用.结论:栀子黄色素具有改善睡眠作用.
목적:탐심치자중구유개선수면작용적활성성분.방법:건강ICR소서,분설치자감저제량조(65mg/kg bw)、치자감중제량조(650mg/kg bw)、치자감고제최조(1950mg/kg bw),치료황색소저제량조(30mg/kg bw)、치자황색소중제량조(300mg/kg bw)、치자황색소고제량조(900mg/kg bw)화공백대조조(증류수),련속관위28d후,측정각항수면지표.결과:여대조조비교,치자황색소저、중、고제량조분별장소서수면잠복기축단료18.2%(P<0.05)、21.4%(P<0.01)、26.2%(P<0.01);동시,장소서수면시간분별연장료32.4%(P<0.05)、48.1%(P<0.01)、52.4%(P<0.01).치자감대수면무명현적개선작용.결론:치자황색소구유개선수면작용.
Cape Jasmine Fruit was extracted with 75% ethanol in a 27.0 kHz ultrasonic field, and the extract was defatted by extraction with petroleum ether and purified by extraction with ethyl acetate followed by water saturated n-butanol. Finally, geniposide and gardenia yellow were separated from the extract by preparative HPLC. The effects of the geniposide and gardenia yellow obtained on sleep latency period and sleep time of mice were investigated to evaluate their sedative activities. Healthy ICR mice were randomly divided into six treatment groups including geniposide-low (65 mg/kg bw), geniposide-medium (650 mg/kg bw), geniposide-high (1950 mg/kg bw), gardenia yellow-low (30 mg/kg bw), gardenia yellow-medium (300 mg/kg bw), gardenia yellow-high (900 mg/kg bw) groups and one control group (administered with distilled water). All the mice were administered by gavage for 28 days. The sleep latency periods of the gardenia yellow-low, gardenia yellow -medium and gardenia yellow-high groups were shorter than those of the control group by 18.2% (P < 0.05), 21.4% (P < 0.01) and 26.2% (P < 0.01), respectively. Meanwhile, the sleep time was also prolonged by 32.4% (P < 0.05), 48.1% (P < 0.01) and 52.4% (P < 0.01), respectively. These results demonstrate that the gardenia yellow from Cape Jasmine Fruit possesses potent sedative activity.