CAJ | 학술논문

目的探讨PTEN与Survivin在血管瘤发生、发展过程中的表达及意义.方法应用免疫组织化学方法和RT-PCR法检测了皮肤血管瘤增生期、退化期以及正常皮肤组织中PTEN和Survivin的表达水平.结果 ①免疫组织化学结果:PTEN在增生期血管瘤内皮细胞的表达低于退化期,差异有显著性意义(P<0.05);退化期血管瘤内皮细胞PTEN表达水平高于正常皮肤组织,差异有显著性意义(P<0.05).Survivin在增生期血管瘤内皮细胞的表达明显高于退化期血管瘤内皮细胞和正常皮肤组织血管内皮细胞(均P<0.01),而Survivin在正常皮肤组织血管内皮细胞的表达与退化期血管瘤内皮细胞的表达差异无显著性意义(P>0.05).②RT-PCR结果:在退化期血管瘤和正常皮肤组织中均有明显的PTEN mRNA表达,而增生期血管瘤中PTEN mRNA的表达较弱;在增生期血管瘤中有明显的Survivin mRNA表达,而退化期血管瘤和正常皮肤组织中Survivin mRNA均无表达.结论 PTEN和Survivin参与了血管瘤的发生、发展和退化,PTEN通过诱导内皮细胞凋亡而促进血管瘤由增生向退化的转变,Survivin通过抑制内皮细胞凋亡而促进血管瘤的增生,两者在血管瘤的发生发展中发挥协同效应.
목적 탐토PTEN여Survivin재혈관류발생、발전과정중적표체급의의.방법 응용면역조직화학방법화RT-PCR법검측료피부혈관류증생기、퇴화기이급정상피부조직중PTEN화Survivin적표체수평.결과 ①면역조직화학결과:PTEN재증생기혈관류내피세포적표체저우퇴화기,차이유현저성의의(P<0.05);퇴화기혈관류내피세포PTEN표체수평고우정상피부조직,차이유현저성의의(P<0.05).Survivin재증생기혈관류내피세포적표체명현고우퇴화기혈관류내피세포화정상피부조직혈관내피세포(균P<0.01),이Survivin재정상피부조직혈관내피세포적표체여퇴화기혈관류내피세포적표체차이무현저성의의(P>0.05).②RT-PCR결과:재퇴화기혈관류화정상피부조직중균유명현적PTEN mRNA표체,이증생기혈관류중PTEN mRNA적표체교약;재증생기혈관류중유명현적Survivin mRNA표체,이퇴화기혈관류화정상피부조직중Survivin mRNA균무표체.결론 PTEN화Survivin삼여료혈관류적발생、발전화퇴화,PTEN통과유도내피세포조망이촉진혈관류유증생향퇴화적전변,Survivin통과억제내피세포조망이촉진혈관류적증생,량자재혈관류적발생발전중발휘협동효응.
Objective To study the expression of PTEN and Survivin,and investigate the mechanism and significance of them in different phases of human hemangiomas. Methods The expression of PTEN and Survivin was detected by using immu-no-histochemical technique,and that of PTEN mRNA and Survivin mRNA by using reverse transcription polymerase chain reac-tion(RT-PCR)in proliferating,involuting human hemangiomas and normal skin tissues. Results ①The expression of PTEN in the endothelial cells of involuting hemangiomas was significantly higher than in proliferating hemangiomas(P<0. 05)and normal skin tissues(P<0. 05). The expression of PTEN in the endothelial cells of involuting hemangiomas was higher than in normal skin tissues(P<0. 05). The expression of Survivin was significantly higher in the endothelial cells of proliferating hemangiomas than in involuting hemangiomas and normal skin tissues(P<0. 01) ,but there was no significant difference in the expression of Survivin between the endothelial cells in involuting hemangiomas and normal skin tissues(P>0. 05). ②The expression of PTEN mRNA was strong in involting hemangiomas and normal skin tissues, but weak in proliferating hemangiomas. The expression of Survivin mRNA was significantly increased in proliferating hemangiomas,and no Survivin mRNA was detected in involuting hemangiomas and normal skin tissues. Conclusion It is suggested that both PTEN and Survivin may take part in the genesis,development,and involution. PTEN promoted the switching from proliferation to involution in hemangiomas through inducing the apoptosis of endothelial cells. Survivin accelerated the proliferation of hemangiomas by inhibiting the apoptosis of endothelial cells.