CAJ | 학술논문

建立构树DNA提取方法及最佳SRAP-PCR反应体系,用SRAP分子标记研究构树种质资源的遗传多样性.结果表明:SRAP扩增的清晰条带大小在100～1 000 bp,用17对引物对21份材料扩增出439条带,其中多态性条带319条,占72.6%,Shannon信息指数(I)均值为0.227 5,物种水平的Nei基因多样性(H)均值为0.133 6,表明构树的遗传多样性不高.聚类分析结果显示与地理分布有相关性.同时发现一些特异性条带,可以转化为SCAR标记,这为构树的遗传图谱构建、遗传育种等研究提供了参考.
건립구수DNA제취방법급최가SRAP-PCR반응체계,용SRAP분자표기연구구수충질자원적유전다양성.결과표명:SRAP확증적청석조대대소재100～1 000 bp,용17대인물대21빈재료확증출439조대,기중다태성조대319조,점72.6%,Shannon신식지수(I)균치위0.227 5,물충수평적Nei기인다양성(H)균치위0.133 6,표명구수적유전다양성불고.취류분석결과현시여지리분포유상관성.동시발현일사특이성조대,가이전화위SCAR표기,저위구수적유전도보구건、유전육충등연구제공료삼고.
Broussonetia papyrifera is important with its environmental protection value and economic value. In this report,genetic diversity and genetic relationship were analyzed by SRAP marker. A DNA extraction protocol was developed for B.papyrifera and then an optimal SRAP-PCR system was established. The results showed that the bands ranged from 100 to 1 000 bp were clearly displayed. A total 439 bands were obtained by 17 pairs of primers, among which 319 bands were polymorphic,accounting for more than 72.6% of the total bands. The average Nei's genetic diversity and Shannon's information diversity index were respectively 0.227 5 and 0.133 6, which indicated genetic diversity of B. papyrifera was not high. The cluster analysis revealed there was relationship between the genetic variation and geographical distribution of B. papyrifera . Some specific bands were founded and could be converted into SCAR markers. This research would provide reference for making genetic map and breeding in B. papyrifera.