CAJ | 학술논문

目的:建立一种耐药性稳定的人涎腺黏液表皮样癌裸鼠移植瘤模型.方法:将人涎腺黏液表皮样癌多药耐药细胞接种于裸鼠皮下,成瘤后经腹腔注射小剂量5-FU进行体内诱导,建立稳定的黏液表皮样癌裸鼠移植瘤模型.结果:裸鼠移植瘤原代培养的MEC/5-FU/NU细胞与体外培养细胞结构类似,MEC/5-FU/NU细胞对5-FU的耐药指数为27.82.RT-PCR显示耐药相关基因ABCB1、ABCB11、GSTA1在MEC/5-FU/NU细胞中明显高表达(P<0.05).免疫荧光显示多药耐药蛋白MDR-1在MEC/5-FU/NU细胞中高表达.结论:以MEC/5-FU建立的黏液表皮样癌裸鼠皮下移植瘤模型仍保持MEC/5-FU细胞的形态学特征和多药耐药性,可为耐药性人涎腺黏液表皮样癌耐药机制和逆转的研究提供较理想的动物模型.
목적:건립일충내약성은정적인연선점액표피양암라서이식류모형.방법:장인연선점액표피양암다약내약세포접충우라서피하,성류후경복강주사소제량5-FU진행체내유도,건립은정적점액표피양암라서이식류모형.결과:라서이식류원대배양적MEC/5-FU/NU세포여체외배양세포결구유사,MEC/5-FU/NU세포대5-FU적내약지수위27.82.RT-PCR현시내약상관기인ABCB1、ABCB11、GSTA1재MEC/5-FU/NU세포중명현고표체(P<0.05).면역형광현시다약내약단백MDR-1재MEC/5-FU/NU세포중고표체.결론:이MEC/5-FU건립적점액표피양암라서피하이식류모형잉보지MEC/5-FU세포적형태학특정화다약내약성,가위내약성인연선점액표피양암내약궤제화역전적연구제공교이상적동물모형.
Objective:To establish a multidrug resistant model of human mucoepidermoid carcinoma using nude mice. Methods:Multidrug resistant MEC/5-FU cells were inoculated intradermally into nude mice. Solid tumors were locally measurable after 10 days and 5-FU was repeated intraperitoneal injected into tumor-bearing mice. The tumor cells in nude mice (MEC/5-FU/NU) were isolated, cultured and examined. Results:The xenografts were similar to the original mucoepidermoid carcinoma from which the cell line was derived. The resistance index (RI) of the MEC/5-FU/NU cells to 5-FU was 27.82. Compared to the MEC, the expressions of ABCB1, ABCB11 and GSTA1 genes and MDR-1 protein increased in the MEC/5-FU/NU cells(P<0.05). Conclusion:The xenograft model is a good model of human multidrug resistant mucoepidermoid carcinoma, and may be useful in studying drug resistance mechanism in vivo.