中国组织工程研究与临床康复
中國組織工程研究與臨床康複
중국조직공정연구여림상강복
JOURNAL OF CLINICAL REHABILITATIVE TISSUE ENGINEERING RESEARCH
2010年
12期
2137-2140
,共4页
郝玉全%高莹娇%秦小梅%韩雪松%刘敏达%艾红军
郝玉全%高瑩嬌%秦小梅%韓雪鬆%劉敏達%艾紅軍
학옥전%고형교%진소매%한설송%류민체%애홍군
微晶玻璃%生物安全性%急性全身毒性实验%小鼠%生物材料
微晶玻璃%生物安全性%急性全身毒性實驗%小鼠%生物材料
미정파리%생물안전성%급성전신독성실험%소서%생물재료
背景:自主设计的可切削生物活性微晶玻璃,在保证抗弯强度的同时,有效降低了熔制温度、制备成本.在临床应用前,除必要的机械、理化性能研究外,还需进行生物相容性评估.目的:观察新型可切削生物活性微晶玻璃对小鼠有无全身毒性作用,评价其生物安全性.方法:将纯系昆明种小鼠随机分为实验组、阴性对照组和阳性对照组,实验组经口灌注可切削生物活性微晶玻璃的阿拉伯胶混悬液,阴性对照组灌注等体积阿拉伯胶,阳性对照组灌注醋酸铅溶液,于灌注后24,48,72 h观察小鼠一般状态、毒性表现并记录体质量变化.最后处死小鼠取出肝、肾,做光、电镜检查.结果与结论:72 h内,实验组小鼠体质量未下降,与阴性对照组比较,差异无显著性意义(P>0.05),肝、肾组织未见病理改变.提示该新型可切削生物活性微晶玻璃经短期口服后对机体无全身毒性.
揹景:自主設計的可切削生物活性微晶玻璃,在保證抗彎彊度的同時,有效降低瞭鎔製溫度、製備成本.在臨床應用前,除必要的機械、理化性能研究外,還需進行生物相容性評估.目的:觀察新型可切削生物活性微晶玻璃對小鼠有無全身毒性作用,評價其生物安全性.方法:將純繫昆明種小鼠隨機分為實驗組、陰性對照組和暘性對照組,實驗組經口灌註可切削生物活性微晶玻璃的阿拉伯膠混懸液,陰性對照組灌註等體積阿拉伯膠,暘性對照組灌註醋痠鉛溶液,于灌註後24,48,72 h觀察小鼠一般狀態、毒性錶現併記錄體質量變化.最後處死小鼠取齣肝、腎,做光、電鏡檢查.結果與結論:72 h內,實驗組小鼠體質量未下降,與陰性對照組比較,差異無顯著性意義(P>0.05),肝、腎組織未見病理改變.提示該新型可切削生物活性微晶玻璃經短期口服後對機體無全身毒性.
배경:자주설계적가절삭생물활성미정파리,재보증항만강도적동시,유효강저료용제온도、제비성본.재림상응용전,제필요적궤계、이화성능연구외,환수진행생물상용성평고.목적:관찰신형가절삭생물활성미정파리대소서유무전신독성작용,평개기생물안전성.방법:장순계곤명충소서수궤분위실험조、음성대조조화양성대조조,실험조경구관주가절삭생물활성미정파리적아랍백효혼현액,음성대조조관주등체적아랍백효,양성대조조관주작산연용액,우관주후24,48,72 h관찰소서일반상태、독성표현병기록체질량변화.최후처사소서취출간、신,주광、전경검사.결과여결론:72 h내,실험조소서체질량미하강,여음성대조조비교,차이무현저성의의(P>0.05),간、신조직미견병리개변.제시해신형가절삭생물활성미정파리경단기구복후대궤체무전신독성.
BACKGROUND: The machinable bioactive glass-ceramic we designed recently have a lower melting temperature, and preparation of cost while bending strength in ensuring the same time. Prior to clinical application, biocompatibility should be evaluated.OBJECTIVE: To observe the new machinable bioactive glass-ceramics on the availability of systemic toxicity in mice and evaluate its biological security.METHODS: The Kunming mice were randomly divided into an experimental group, negative control group and positive control group. Mice in experimental group were orally perfused with the suspension of machinable bioactive glass-ceramics and Arabic gum. While the mice in the negative control group were treated with an equal volume of Arabic gum, and the mice in the positive control group were treated with an equal volume of lead acetate solution. The general state and toxicity of mice were observed and body mass was recorded after 24 hours, 48 hours, and 72 hours. Finally the mice were sacrificed, and the morphological changes in liver and kidney were then examined by light and electron microscopy.RESULTS AND CONCLUSION: The body mass of mice in the experimental group was not decreased significantly compared with the mice in the negative control group in 72 hours (P> 0.05). Pathological changes of liver and kidney in the experimental group did not be found. The new machinable bioactive glass-ceramic did not show systemic toxicity for a short term.