天然产物研究与开发
天然產物研究與開髮
천연산물연구여개발
NATURAL PRODUCT RESEARCH AND DEVELOPMENT
2010年
4期
564-567
,共4页
徐利剑%李培琴%赵江林%单体江%阴春晖%王明安%周立刚
徐利劍%李培琴%趙江林%單體江%陰春暉%王明安%週立剛
서리검%리배금%조강림%단체강%음춘휘%왕명안%주립강
内生真菌%芬芳镰刀菌%盾叶薯蓣%镰刀菌酸%9%10-脱氢镰刀菌酸%抗菌活性
內生真菌%芬芳鐮刀菌%盾葉藷蕷%鐮刀菌痠%9%10-脫氫鐮刀菌痠%抗菌活性
내생진균%분방렴도균%순협서여%렴도균산%9%10-탈경렴도균산%항균활성
endophytic fungus%Fusarium redolens%Dioscorea zingiberensis%fusaric acid%9,10dehydrofusaric acid%antimicrobial activity
采用活性追踪的方法从盾叶薯蓣内生芬芳镰刀菌Dzf2中分离到两个抗菌活性成分,通过物理化学性质和波谱学特征鉴定为镰刀菌酸(1)和9,10-脱氢镰刀菌酸(2).采用多孔板-MTT-比色法和孢子萌发法测定了化合物的抗菌活性.镰刀菌酸和9,10-脱氢镰刀菌酸对供试细菌的半抑制浓度(IC50)值为35.35 μg/mL至171.29 μg/mL;对稻瘟菌孢子萌发的IC50值分别为28.83 μg/mL和27.06 μg/mL.
採用活性追蹤的方法從盾葉藷蕷內生芬芳鐮刀菌Dzf2中分離到兩箇抗菌活性成分,通過物理化學性質和波譜學特徵鑒定為鐮刀菌痠(1)和9,10-脫氫鐮刀菌痠(2).採用多孔闆-MTT-比色法和孢子萌髮法測定瞭化閤物的抗菌活性.鐮刀菌痠和9,10-脫氫鐮刀菌痠對供試細菌的半抑製濃度(IC50)值為35.35 μg/mL至171.29 μg/mL;對稻瘟菌孢子萌髮的IC50值分彆為28.83 μg/mL和27.06 μg/mL.
채용활성추종적방법종순협서여내생분방렴도균Dzf2중분리도량개항균활성성분,통과물이화학성질화파보학특정감정위렴도균산(1)화9,10-탈경렴도균산(2).채용다공판-MTT-비색법화포자맹발법측정료화합물적항균활성.렴도균산화9,10-탈경렴도균산대공시세균적반억제농도(IC50)치위35.35 μg/mL지171.29 μg/mL;대도온균포자맹발적IC50치분별위28.83 μg/mL화27.06 μg/mL.
Two antimicrobial compounds were obtained by bioassayguided fractionation from the endophytic fungus Fusarium redolens Dzf2 from the medicinal plant Dioscorea zingiberensis.They were identified as fusaric acid(1) and 9,10dehydrofusaric acid(2) by physicochemical and spectral analysis.Antimicrobial activities of the two compounds were evaluated by multimicroplateMTT colorimetric assay and spore germination inhibition assay.Their median effective inhibitory concentration (IC50) values on test bacteria ranged from 35.35 μg/mL to 171.29 μg/mL,and those on the spore germination of Magnaporthe oryzae were 28.83 μg/mL for fusaric acid and 27.06 μg/mL for 9,10dehydrofusaric acid.
