CAJ | 학술논문

将成年健康的小鼠(每组8只)暴露在含有1 500 mg/m3纳米二氧化钛气溶胶密闭装置内,以暴露在空气中的小鼠作为对照,研究了纳米二氧化钛对小鼠肺部组织和血清生化指标的影响.经过不同时间的暴露后将小鼠处死,收集血样并取肺部组织进行生化指标分析和组织病理学检验.血清生化指标检测结果显示,在所有的试验组中,乳酸脱氢酶的活性均提高,丙氨酸氨基转移酶的活性和尿素氮在暴露28天组中增加,肌酐在暴露14天组和28天组中均增加,其他血清学指标未见明显异常.组织病理学检测结果显示,暴露28天的肺泡间隙有纳米粒子存在,并且肺部炎症逐渐增强.
장성년건강적소서(매조8지)폭로재함유1 500 mg/m3납미이양화태기용효밀폐장치내,이폭로재공기중적소서작위대조,연구료납미이양화태대소서폐부조직화혈청생화지표적영향.경과불동시간적폭로후장소서처사,수집혈양병취폐부조직진행생화지표분석화조직병이학검험.혈청생화지표검측결과현시,재소유적시험조중,유산탈경매적활성균제고,병안산안기전이매적활성화뇨소담재폭로28천조중증가,기항재폭로14천조화28천조중균증가,기타혈청학지표미견명현이상.조직병이학검측결과현시,폭로28천적폐포간극유납미입자존재,병차폐부염증축점증강.
The effect of inhaled nano-TiO2 on lung histology and serum biochemical indexes is evaluated in healthy and adult Kunming mice (eight in each group) after exposure to TiO2 aerosols (1 500 mg/m3) in a sealed chamber. Another eight mice are exposed to indoor air to be served as a control group. Lung tissue and blood are collected after euthanizing the animals. The results show that lactate dehydrogenase (LDH) activity increases in all experimental groups. Alanine aminotransferase(ALT) activity and blood urea nitrogen (BUN) levels are increased in the group exposed to TiO2 aerosols for 28 d, and creatinine(Cr) levels are increased in 14 d and 28 d samples (P＜0.05). No obvious changes are observed in other serum indexes. Lungs of mice exposed to 28 d exposure show significant but moderate increase in pulmonary inflammation, and many TiO2 particles are found in the interstitium of pulmonary alveoli.