中华细胞与干细胞杂志（电子版）
中華細胞與榦細胞雜誌（電子版）
중화세포여간세포잡지（전자판）
CHINESE JOURNAL OF CELL AND STEM CELL
2012年
1期
27-30
,共4页
邱建新%蔡俊超%Paul I.Terasaki
邱建新%蔡俊超%Paul I.Terasaki
구건신%채준초%Paul I.Terasaki
HLA抗原%抗体%肾移植%移植物排斥
HLA抗原%抗體%腎移植%移植物排斥
HLA항원%항체%신이식%이식물배척
HLA antigens%Antibodies%Kidney transplantation%Graft Rejection
目的 探讨采用单抗原磁珠法检测肾移植患者的人类白细胞抗原DP (HLA-DP)抗体的可行性,及其与移植肾功能的关系.方法 采用流式细胞学单抗原磁珠法检测了323例患者的血清,分为有功能组138例,排斥组185例,分别测定DPw1 (DP0101)、DPw2 (DP0201)、DPw3 (DP0301)、DPw4 (DP0401)、DPw5 (DP0501)和DPw11 (DP1101)6种DP抗原值,两组各抗原值的差异比较采用卡方检验.结果 在138例有功能组的肾移植患者血清中有(7/138)5.1％检测到HLA-DP抗体,而排斥组的185例患者血清中有(36/185)19.5％检测到HLA-DP抗体(x2=14.176,P=0.000).在有功能组和排斥组中分别有(3/7)42.9％和(23/86) 63.9％的H1A-DP抗体阳性患者同时有DR/DQ抗体.在没有HLA-I类及DR/DQ抗体的患者中,在排斥组中有14.3％的患者可检测出HLA-DP抗体,而有功能组中只有3.5％有HLA-DP抗体(x2=7.165,P=0.007).在排斥组中,DP0301的检出频率最高.结论 相对于有功能组来说,HLA-DP抗体在排斥组中有更高的检出率.特别是对于再次移植的患者来说,应行HLA-DP组织配型以避免HLA-DP等位基因引起的致敏.
目的 探討採用單抗原磁珠法檢測腎移植患者的人類白細胞抗原DP (HLA-DP)抗體的可行性,及其與移植腎功能的關繫.方法 採用流式細胞學單抗原磁珠法檢測瞭323例患者的血清,分為有功能組138例,排斥組185例,分彆測定DPw1 (DP0101)、DPw2 (DP0201)、DPw3 (DP0301)、DPw4 (DP0401)、DPw5 (DP0501)和DPw11 (DP1101)6種DP抗原值,兩組各抗原值的差異比較採用卡方檢驗.結果 在138例有功能組的腎移植患者血清中有(7/138)5.1％檢測到HLA-DP抗體,而排斥組的185例患者血清中有(36/185)19.5％檢測到HLA-DP抗體(x2=14.176,P=0.000).在有功能組和排斥組中分彆有(3/7)42.9％和(23/86) 63.9％的H1A-DP抗體暘性患者同時有DR/DQ抗體.在沒有HLA-I類及DR/DQ抗體的患者中,在排斥組中有14.3％的患者可檢測齣HLA-DP抗體,而有功能組中隻有3.5％有HLA-DP抗體(x2=7.165,P=0.007).在排斥組中,DP0301的檢齣頻率最高.結論 相對于有功能組來說,HLA-DP抗體在排斥組中有更高的檢齣率.特彆是對于再次移植的患者來說,應行HLA-DP組織配型以避免HLA-DP等位基因引起的緻敏.
목적 탐토채용단항원자주법검측신이식환자적인류백세포항원DP (HLA-DP)항체적가행성,급기여이식신공능적관계.방법 채용류식세포학단항원자주법검측료323례환자적혈청,분위유공능조138례,배척조185례,분별측정DPw1 (DP0101)、DPw2 (DP0201)、DPw3 (DP0301)、DPw4 (DP0401)、DPw5 (DP0501)화DPw11 (DP1101)6충DP항원치,량조각항원치적차이비교채용잡방검험.결과 재138례유공능조적신이식환자혈청중유(7/138)5.1％검측도HLA-DP항체,이배척조적185례환자혈청중유(36/185)19.5％검측도HLA-DP항체(x2=14.176,P=0.000).재유공능조화배척조중분별유(3/7)42.9％화(23/86) 63.9％적H1A-DP항체양성환자동시유DR/DQ항체.재몰유HLA-I류급DR/DQ항체적환자중,재배척조중유14.3％적환자가검측출HLA-DP항체,이유공능조중지유3.5％유HLA-DP항체(x2=7.165,P=0.007).재배척조중,DP0301적검출빈솔최고.결론 상대우유공능조래설,HLA-DP항체재배척조중유경고적검출솔.특별시대우재차이식적환자래설,응행HLA-DP조직배형이피면HLA-DP등위기인인기적치민.
Objective Here HLA-DP antibodies were detected with single antigen beads to evaluate the effects of HLA-DP on renal graft function.Methods We studied 323 sera of patients from four different centers with functioning or rejected kidney grafts.Recipients were divided into functional group (n=138) and rejection group (n=185).Pw1(DP0101),DPw2(DP0201),DPw3(DP0301),DPw4(DP0401),DPw5(DP0501) and DPw11(DP1101) were assayed.The results were compared with Chi-square test.Results DP antibodies were found in 5.1％ of 138 patients with functioning grafts,and 19.5％of 185 patients with rejected grafts (x2=14.176,P =0.000).In the functional group,42.9％ patients were positive for both DP antibody and DR/DQ antibodyies.In the rejection group,63.9 ％patients were positive for both DP antibody and DR/DQ antibody.Among patients who was in the rejection group and did not have Class I and DR/DQ antibodies,14.3 ％had DP antibody,compared to 3.5％ of patients with functioning grafts (x2=7.165,P =0.007).DP 0301 had the highest frequency in the rejection group.Conclusions HLA-DP antibodies were detected at a higher frequency in patients who have rejected their grafts than those with functioning grafts.DP tissue typing is recommended to avoid presensitized DP alleles if second transplantation is performed.
