CAJ | 학술논문

目的评价新犁低弹性β钛合金关节表面微假体治疗犬膝关节软骨面全层缺损的可行性与效果.方法取8只成年家犬,制备舣侧股骨髁直径为7 mm的软骨面全层缺损,选用两种弹性模量钛合金材料制备关节表而微假体并植入软骨缺损区,左侧为低弹性模量组(弹性模量为42 GPa),右侧为高弹性模量组(弹性模量为110 GPa).3个月后取材,行X线片、显微CT扫描、骨计量学分析和组织学检测观察两组关节表面微假体的稳定性及周边骨与软骨的生长情况.结果 X线片示关节表面微假体钉帽周围软骨生长良好,完全填充钉帽周边软骨间隙,关节软骨面平整.显微CT三维重建及骨计量学分析显示低弹性模量组骨体积分数、骨小梁厚度、骨小梁数目及骨组织密度平均分别为0.389%±0.025%、(0.049±0.002)μm、(8.9±0.4)mm1、(652.7±12.6)mg/mm3,而高弹性模量组平均分别为0.253%±0.024%、(0.038 ±0.002)μm、(5.9±0.4)mm-1、(595.2±7.6)mg/mm3,以上指标两组间比较差异均有统计学意义(P<0.05).组织学观察显示低弹性模量组微关节表面假体固定钉周围有更多的新生骨小梁包绕,钉骨结合牢固,界面成骨量明显多于高弹性模量组.结论新型低弹性β钛合金关节表面微假体治疗关节软骨面缺损是可行的,其低弹性模量的特点更有利干钉骨界面骨的形成.
목적 평개신리저탄성β태합금관절표면미가체치료견슬관절연골면전층결손적가행성여효과.방법 취8지성년가견,제비의측고골과직경위7 mm적연골면전층결손,선용량충탄성모량태합금재료제비관절표이미가체병식입연골결손구,좌측위저탄성모량조(탄성모량위42 GPa),우측위고탄성모량조(탄성모량위110 GPa).3개월후취재,행X선편、현미CT소묘、골계량학분석화조직학검측관찰량조관절표면미가체적은정성급주변골여연골적생장정황.결과 X선편시관절표면미가체정모주위연골생장량호,완전전충정모주변연골간극,관절연골면평정.현미CT삼유중건급골계량학분석현시저탄성모량조골체적분수、골소량후도、골소량수목급골조직밀도평균분별위0.389%±0.025%、(0.049±0.002)μm、(8.9±0.4)mm1、(652.7±12.6)mg/mm3,이고탄성모량조평균분별위0.253%±0.024%、(0.038 ±0.002)μm、(5.9±0.4)mm-1、(595.2±7.6)mg/mm3,이상지표량조간비교차이균유통계학의의(P<0.05).조직학관찰현시저탄성모량조미관절표면가체고정정주위유경다적신생골소량포요,정골결합뢰고,계면성골량명현다우고탄성모량조.결론 신형저탄성β태합금관절표면미가체치료관절연골면결손시가행적,기저탄성모량적특점경유리간정골계면골적형성.
Objective To assess the effect of a new β Ti alloy mini-prosthesis with low elastic modulus on resurfacing the full-thickness Surface defect of the knee joint in dogs.Methods A full-thickness osteochondral defect of 7 mm in diameter was created at the medial femoral condyle of both hind limbs in 8 healthy adult dogs.The titanium alloy mini-prostheses with high-modulus of elasticity were implanted on the right side(control group)and those with low-modulus of elasticity on the left side(experiment group)to repair the cartilage defects.After 3 months,all the 8 dogs were sacrificed to harvest the specimens.Radiographic,histologic and micro-CT examinations were conducted to observe stability of the mini-prostheses and growth of the bone and cartilage surrounding the implants. Results Radiographic evaluation revealed no indications of device disassembly or subsidence.Bone trabeculae surrounding anchoring screws in the experiment group was visibly more and denser compared with the control group.Micro-CT data revealed that the bone volume fraction,trabecular thickness,trabecular number and tissue mineral density were 0.389%±0.025%,0.049±0.002 μm,8.9±0.4 mm-1 and 652.7 ±12.6 mg/mm3 respectively in the experiment group,compare with 0.253%±0.024%,0.038±0.002 μm,5.9±0.4 mm-1 and 595.2±7.6 mg/mm3 in the control group,with statistically significant differences between the 2 groups(P<0.05).Cartilage around the cap grew well and the surface remained smooth.Periphery of the resurfacing implant was covered by an extension of the superficial cartilage emanating from the defect margins. Conclusions The new β Ti alloy mini-prosthesis with low elastic modulus is favorable toward resurfacing the articular surface defect.The low-modulus of elasticity can improve new bone formation on the implant-bone interface.