中华航海医学与高气压医学杂志
中華航海醫學與高氣壓醫學雜誌
중화항해의학여고기압의학잡지
CHINESE JOURNAL OF NAUTICAL MEDICINE AND HYPERBARIC MEDICINE
2009年
6期
357-359
,共3页
查晓冰%汪昕%郭美奂%钟锦绣
查曉冰%汪昕%郭美奐%鐘錦繡
사효빙%왕흔%곽미환%종금수
高压氧%脑缺血%炎性因子%脑海马神经元
高壓氧%腦缺血%炎性因子%腦海馬神經元
고압양%뇌결혈%염성인자%뇌해마신경원
Hyperbaric oxygen%Cerebral ischemia%Inflammatory cytokine%Hippocampal neurons
目的 探讨高压氧对缺氧缺血性脑损伤(hypoxia-ischemia brain damage,HIBD)大鼠神经细胞凋亡以及炎性细胞因子水平的影响.方法 成年雄性SD大鼠42只,随机分为对照组(6只)、空气加压组(18只)和高压氧(hyperbaric oxyen,HBO)治疗组(18只).采用Pulsineli法制作脑缺血大鼠模型.HBO治疗组第1、3、5天分别给予HBO治疗,1次/d.应用TUNEL染色检测大鼠海马神经元数量,同时检测大鼠细胞因子TNF、IL-1β和IL-6水平.结果 HBO治疗能有效抑制脑缺血引起的细胞因子TNF、IL-1β、IL-6释放(P<0.05或0.01),并减轻大鼠海马区神经元损伤,组间比较差异有统计学意义(P<0.05).结论 HBO治疗可以有效抑制炎性细胞因子的释放,从而保护脑缺血后神经细胞.
目的 探討高壓氧對缺氧缺血性腦損傷(hypoxia-ischemia brain damage,HIBD)大鼠神經細胞凋亡以及炎性細胞因子水平的影響.方法 成年雄性SD大鼠42隻,隨機分為對照組(6隻)、空氣加壓組(18隻)和高壓氧(hyperbaric oxyen,HBO)治療組(18隻).採用Pulsineli法製作腦缺血大鼠模型.HBO治療組第1、3、5天分彆給予HBO治療,1次/d.應用TUNEL染色檢測大鼠海馬神經元數量,同時檢測大鼠細胞因子TNF、IL-1β和IL-6水平.結果 HBO治療能有效抑製腦缺血引起的細胞因子TNF、IL-1β、IL-6釋放(P<0.05或0.01),併減輕大鼠海馬區神經元損傷,組間比較差異有統計學意義(P<0.05).結論 HBO治療可以有效抑製炎性細胞因子的釋放,從而保護腦缺血後神經細胞.
목적 탐토고압양대결양결혈성뇌손상(hypoxia-ischemia brain damage,HIBD)대서신경세포조망이급염성세포인자수평적영향.방법 성년웅성SD대서42지,수궤분위대조조(6지)、공기가압조(18지)화고압양(hyperbaric oxyen,HBO)치료조(18지).채용Pulsineli법제작뇌결혈대서모형.HBO치료조제1、3、5천분별급여HBO치료,1차/d.응용TUNEL염색검측대서해마신경원수량,동시검측대서세포인자TNF、IL-1β화IL-6수평.결과 HBO치료능유효억제뇌결혈인기적세포인자TNF、IL-1β、IL-6석방(P<0.05혹0.01),병감경대서해마구신경원손상,조간비교차이유통계학의의(P<0.05).결론 HBO치료가이유효억제염성세포인자적석방,종이보호뇌결혈후신경세포.
Objective To investigate effect of hyperbaric oxygen (HBO) on inflammatory cytokine and neuronal apoptosis in rats with hypoxia ischemia brain damage (HIBD). Methods Forty-two adult male Sprague-Dawley rats were randomly divided into 7 groups (n=6 respectively) following ischemia-hypotension:the blank control group, the ischemia-hypotension 1, 3 and 5 d groups, and the HBO treatment 1, 3 and 5 d groups. The global I/R model was developed with Pulsineli 4 vessel occlusion method. Rats in the HBO treatment groups were exposed to HBO for 1 h a day for a duration of 1 to 5 days. TUNEL staining was used for neuron counting and ELISA was employed to detect levels of TNF, IL-1β and IL-6 in serum. Results Levels of TNF, IL-1β and IL-6 in the HBO treatment groups were lower than those in the untreated groups. After 5 days of HBO treatment, number of neurons in the hippocampal area was significantly higher than that in the untreated group (P <0.05). Conclusions HBO could decrease levels of TNF, IL-1β and IL-6 induced by cerebral ischemia,which reduces the loss of hippocampal neurons after global ischemia-hypotension.
