中华普通外科杂志
中華普通外科雜誌
중화보통외과잡지
CHINESE JOURNAL OF GENERAL SURGERY
2012年
4期
322-325
,共4页
杨莉%魏玲%赵伟%宋现让%王兴武%郑刚%郑美珠%左文述
楊莉%魏玲%趙偉%宋現讓%王興武%鄭剛%鄭美珠%左文述
양리%위령%조위%송현양%왕흥무%정강%정미주%좌문술
乳腺肿瘤%骨桥蛋白质%缺氧%RNA干扰
乳腺腫瘤%骨橋蛋白質%缺氧%RNA榦擾
유선종류%골교단백질%결양%RNA간우
Breast neoplasms%Osteopontin%Anoxia%RNA interference
目的 观察乳腺癌细胞MDA-MB-231 osteopontin (OPN) RNA靶向干扰效果.方法 采用OPN mRNA干扰质粒转染MDA-MB-231细胞,建立OPN基因沉默细胞株,采用RT-PCR、蛋白质印迹法从mRNA和蛋白水平检测乳腺癌细胞OPN表达量的变化,侵袭试验检测OPN下调对细胞侵袭能力的影响,MTT法测细胞增殖能力的改变,裸鼠成瘤后观察OPN RNA干扰对肿瘤细胞生长的影响及对移植瘤乏氧程度的影响.结果 与MDA-MB-231细胞相比,OPN沉默细胞在常氧和乏氧状态下OPN表达均显著下调(均P<0.05),OPN稳定沉默细胞侵袭力和增殖能力下降(均P<0.01),OPN RNAi能明显抑制裸鼠移植瘤的生长(P<0.05),且移植瘤内乏氧程度降低(P<0.05).结论 OPN RNA干扰可抑制乳腺癌细胞MDA-MB-231细胞侵袭力和增殖能力,降低移植瘤的乏氧程度.
目的 觀察乳腺癌細胞MDA-MB-231 osteopontin (OPN) RNA靶嚮榦擾效果.方法 採用OPN mRNA榦擾質粒轉染MDA-MB-231細胞,建立OPN基因沉默細胞株,採用RT-PCR、蛋白質印跡法從mRNA和蛋白水平檢測乳腺癌細胞OPN錶達量的變化,侵襲試驗檢測OPN下調對細胞侵襲能力的影響,MTT法測細胞增殖能力的改變,裸鼠成瘤後觀察OPN RNA榦擾對腫瘤細胞生長的影響及對移植瘤乏氧程度的影響.結果 與MDA-MB-231細胞相比,OPN沉默細胞在常氧和乏氧狀態下OPN錶達均顯著下調(均P<0.05),OPN穩定沉默細胞侵襲力和增殖能力下降(均P<0.01),OPN RNAi能明顯抑製裸鼠移植瘤的生長(P<0.05),且移植瘤內乏氧程度降低(P<0.05).結論 OPN RNA榦擾可抑製乳腺癌細胞MDA-MB-231細胞侵襲力和增殖能力,降低移植瘤的乏氧程度.
목적 관찰유선암세포MDA-MB-231 osteopontin (OPN) RNA파향간우효과.방법 채용OPN mRNA간우질립전염MDA-MB-231세포,건립OPN기인침묵세포주,채용RT-PCR、단백질인적법종mRNA화단백수평검측유선암세포OPN표체량적변화,침습시험검측OPN하조대세포침습능력적영향,MTT법측세포증식능력적개변,라서성류후관찰OPN RNA간우대종류세포생장적영향급대이식류핍양정도적영향.결과 여MDA-MB-231세포상비,OPN침묵세포재상양화핍양상태하OPN표체균현저하조(균P<0.05),OPN은정침묵세포침습력화증식능력하강(균P<0.01),OPN RNAi능명현억제라서이식류적생장(P<0.05),차이식류내핍양정도강저(P<0.05).결론 OPN RNA간우가억제유선암세포MDA-MB-231세포침습력화증식능력,강저이식류적핍양정도.
Objective To investigate the role of OPN in human breast cancer cell line ( MDA-MB-231) by using small interfering RNA to specifically knockdown OPN expression. Methods OPN ShRNA expression vector was stably transfected to MDA-MB-231 cell line.The expression of OPN mRNA and protein were analyzed using reverse transcription polymerase chain reaction (RT-PCR) and Western blot,respectively.The growth of MDA-MB-231 cells were observed by MTT.The effect of OPN siRNA on the transplanted tumor growth and tumor hypoxia were assessed in nude mice. Results The expression level of OPN in MDA-MB-231 cells were significantly lower under hypoxia or normoxia(P < 0.05 ).OPN silence with RNAi significantly inhibited the invasion ability and proliferation of MDA-MB-231 cell lines (P < 0.01 ).Inhibition of OPN with RNAi significantly inhibited the growth ability of MDA-MB-231 cells in vivo(P <0.05).The tumor hypoxia significantly decreased(P < 0.05). Conclusions OPN silence with RNAi can effectively inhibit cell proliferation and tumor growth of MDA-MB-231 cells,and decrease the bypoxia level of MDA-MB-231 transplanted tumor in nude mice.