中华皮肤科杂志
中華皮膚科雜誌
중화피부과잡지
Chinese Journal of Dermatology
2010年
5期
342-345
,共4页
人乳头瘤病毒16%乳头瘤病毒E7蛋白质类%T淋巴细胞%细胞毒性%表型
人乳頭瘤病毒16%乳頭瘤病毒E7蛋白質類%T淋巴細胞%細胞毒性%錶型
인유두류병독16%유두류병독E7단백질류%T림파세포%세포독성%표형
Human papillomavirus 16%Papillomavirus E7 proteins%T-Lymphocytes,cytotoxic%Phenotype
目的 探讨高致癌性HPV16E7病毒抗原肽体外诱生HLA-A2阳性正常人外周血抗原特异性CD8+细胞毒性T细胞(CTL)的表型及功能状态.方法 以HPV16E711-20合成多肽、重组人IL-7、IL-2体外刺激26例HLA-A2阳性正常人外周血T细胞,用HLA-A*0201限制性表位肽/YMLDLQPETT五聚体技术,结合流式细胞仪对抗原特异性CTL进行频率、表型[CD45RA+CD27-效应性CTL、CD45RA-CD27-效应性记忆CTL(TEM)、CD45RA-CD27+中枢性记忆CTL(TCM)、CD45+CD27+初始性CTL]及功能(穿孔素、颗粒酶B、FasL)分析,并以无关肽HBVcoxe18-27作为阴性对照.结果 病毒多肽体外刺激T细胞7 d后,抗原特异性CD8+CTL数为(0.73±0.33)%,比未刺激组的(0.02±0.03)%明显增多(P＜0.01).进一步分析,在抗原特异性CTL中,效应性CTL、TEM、TCM及初始性CTL百分比分别为(26.07±13.46)%、(7.97±7.11)%、(33.25±19.68)%及(32.73±13.89)%,均比未刺激组的(0.02±0.03)%、(0.02±0.03)%、(0.02±0.03)%及(0.02±0.05)%明显增多,P值均＜0.01.HPV16E711-20表位特异性CTL分泌穿孔素、颗粒酶B、Fas-L的水平分别为(47.01±18.69)%、(80.53±13.32)%及(26.48±7.81)%,均比未刺激组的(0.38±0.55)%、(0.34±0.22)%及(0.16±0.16)%明显增多,P值均＜0.01.结论 HPV16E711-20多肽诱导CD8+T细胞克隆扩增,产生抗原特异性CD8+CTL,分泌毒性细胞因子,通过不同机制特异性杀伤病毒感染的靶细胞,在抗病毒免疫应答中发挥作用.
目的 探討高緻癌性HPV16E7病毒抗原肽體外誘生HLA-A2暘性正常人外週血抗原特異性CD8+細胞毒性T細胞(CTL)的錶型及功能狀態.方法 以HPV16E711-20閤成多肽、重組人IL-7、IL-2體外刺激26例HLA-A2暘性正常人外週血T細胞,用HLA-A*0201限製性錶位肽/YMLDLQPETT五聚體技術,結閤流式細胞儀對抗原特異性CTL進行頻率、錶型[CD45RA+CD27-效應性CTL、CD45RA-CD27-效應性記憶CTL(TEM)、CD45RA-CD27+中樞性記憶CTL(TCM)、CD45+CD27+初始性CTL]及功能(穿孔素、顆粒酶B、FasL)分析,併以無關肽HBVcoxe18-27作為陰性對照.結果 病毒多肽體外刺激T細胞7 d後,抗原特異性CD8+CTL數為(0.73±0.33)%,比未刺激組的(0.02±0.03)%明顯增多(P＜0.01).進一步分析,在抗原特異性CTL中,效應性CTL、TEM、TCM及初始性CTL百分比分彆為(26.07±13.46)%、(7.97±7.11)%、(33.25±19.68)%及(32.73±13.89)%,均比未刺激組的(0.02±0.03)%、(0.02±0.03)%、(0.02±0.03)%及(0.02±0.05)%明顯增多,P值均＜0.01.HPV16E711-20錶位特異性CTL分泌穿孔素、顆粒酶B、Fas-L的水平分彆為(47.01±18.69)%、(80.53±13.32)%及(26.48±7.81)%,均比未刺激組的(0.38±0.55)%、(0.34±0.22)%及(0.16±0.16)%明顯增多,P值均＜0.01.結論 HPV16E711-20多肽誘導CD8+T細胞剋隆擴增,產生抗原特異性CD8+CTL,分泌毒性細胞因子,通過不同機製特異性殺傷病毒感染的靶細胞,在抗病毒免疫應答中髮揮作用.
목적 탐토고치암성HPV16E7병독항원태체외유생HLA-A2양성정상인외주혈항원특이성CD8+세포독성T세포(CTL)적표형급공능상태.방법 이HPV16E711-20합성다태、중조인IL-7、IL-2체외자격26례HLA-A2양성정상인외주혈T세포,용HLA-A*0201한제성표위태/YMLDLQPETT오취체기술,결합류식세포의대항원특이성CTL진행빈솔、표형[CD45RA+CD27-효응성CTL、CD45RA-CD27-효응성기억CTL(TEM)、CD45RA-CD27+중추성기억CTL(TCM)、CD45+CD27+초시성CTL]급공능(천공소、과립매B、FasL)분석,병이무관태HBVcoxe18-27작위음성대조.결과 병독다태체외자격T세포7 d후,항원특이성CD8+CTL수위(0.73±0.33)%,비미자격조적(0.02±0.03)%명현증다(P＜0.01).진일보분석,재항원특이성CTL중,효응성CTL、TEM、TCM급초시성CTL백분비분별위(26.07±13.46)%、(7.97±7.11)%、(33.25±19.68)%급(32.73±13.89)%,균비미자격조적(0.02±0.03)%、(0.02±0.03)%、(0.02±0.03)%급(0.02±0.05)%명현증다,P치균＜0.01.HPV16E711-20표위특이성CTL분비천공소、과립매B、Fas-L적수평분별위(47.01±18.69)%、(80.53±13.32)%급(26.48±7.81)%,균비미자격조적(0.38±0.55)%、(0.34±0.22)%급(0.16±0.16)%명현증다,P치균＜0.01.결론 HPV16E711-20다태유도CD8+T세포극륭확증,산생항원특이성CD8+CTL,분비독성세포인자,통과불동궤제특이성살상병독감염적파세포,재항병독면역응답중발휘작용.
Objective To investigate the phenotype and function of antigen-specific CD8+ cytotoxic T cells (CTL) from HLA-A2+ healthy human donors induced by high-carcinogenic HPV 16 E7 peptide in vitro.Methods Peripheral blood T cells from 26 HLA-A2+ healthy human donors were incubated with HPV 16E711-20 peptide (HLA-A*0201/YMLDLQPETT), recombinant human interleukin 7 (IL-7) and IL-2 for 7 days to yield antigen-specific CD8+ CTL. Then, four-color flow cytometric analysis was performed to detect the percentage of antigen-specific CD8+ CTL expressing different surface markers including CD45 and CD27. The expression of three intracellular cytokines including perforin, granzyme-B and FasL in the antigen-specific CD8+ CTL was also measured by intracellular flow cytometry. A wrong peptide, HBVcoxe18-27 (FLPSDFFPSV),was used as an isotype control. Results A significant increase was observed in the percentage of antigen-specific CD8+ CTL in peripheral T cells stimulated with HPV 16 E7-peptide compared with the non-stimulated T cells (0.73% ± 0.33% vs 0.02% ± 0.03%, P ＜ 0.01). The percentage of CD45RA+CD27- effector T cells,CD45RA-CD27- effector memory T (TEM) cells, CD45RA-CD27+ central memory T (TCM) cells, and CD45RA+CD27+ naive T cells in antigen-specific CD8+ CTL was 26.07% ± 13.46%, 7.97% ± 7.11%, 33.25% ± 19.68%and 32.73% ± 13.89%, respectively in HPV 16 E7-stimulated group, significantly higher than that in nonstimulated group (0.02% ± 0.03%, 0.02% ± 0.03%, 0.02% ± 0.03% and 0.02% ± 0.05%, all P ＜ 0.01 ). Elevated proportions of perforin-, granzyme-B- and FasL-expressing antigen specific CTL were observed in HPV 16 E7-stimulated group compared with non-stimulated group (47.01% ± 18.69% vs 0.38% ± 0.55%, 80.53% ±13.32% vs 0.34% ± 0.22%, 26.48% ± 7.81% vs 0.16% ± 0.16%, all P ＜ 0.01 ). Conclusions HPV 16 E7peptide could induce the clonal proliferation of CD8+ T cells, generation of antigen-specific CD8+ CTL and secretion of toxic cytokines, finally lead to a highly efficient and specific killing of virus-infected target cells through different mechanisms, hence, it might play a crucial role in antiviral immune responses.
