CAJ | 학술논문

目的检测信号转导和转录激活子1的活化抑制蛋白( PIASl)在急性胰腺炎(AP)大鼠胰腺中的表达,探讨其对AP病情的评估价值.方法 SD大鼠按随机分配表法分为对照组、急性水肿性胰腺炎(AEP)组、急性坏死性胰腺炎(ANP)组,各15只.术后0.5、6、16、24、48、72 h分批处死大鼠,取血清检测C反应蛋白(CRP)、淀粉酶水平,测胰腺组织含水量,行胰腺常规病理检查并评分,采用蛋白质印迹法和免疫组化法检测胰腺组织PIASI表达.记录大鼠72 h生存情况,行Cox多因素风险评估.结果 ANP组16 h时的胰腺病理评分、胰腺含水量、血清CRP和淀粉酶水平分别为(7.70±2.55)分、(2.9l±0.57)％、(0.36 ±0.05) mg/L、(3141±625) U/L,均显著高于AEP组的(2.60±1.04)分、(2.04±0.47)％、(0.24±0.05) mg/L、(1984±637) U/L,亦显著高于对照组的(0.80±0.42)分、(1.21±0.27)％、(0.14±0.03) mg/L、(978±353) U/L(P值＜0.05或＜0.01).ANP组大鼠平均生存时间为(26.4±3.4)h,较AEP组的(57.3±4.2)h和对照组的(71.3±0.5)h显著缩短(P值均＜0.01).ANP组16 h时的胰腺组织PIAS1蛋白表达较AEP组及对照组显著下调(0.10±0.01比0.80±0.07和0.87±0.05,P＜0.01).Cox分析显示PIAS1为影响AP大鼠生存时间的独立因素之一.结论 PIAS1在ANP中低表达,与AP病情严重度呈负相关,可以成为预测病情严重程度及预后的指标.
목적 검측신호전도화전록격활자1적활화억제단백( PIASl)재급성이선염(AP)대서이선중적표체,탐토기대AP병정적평고개치.방법 SD대서안수궤분배표법분위대조조、급성수종성이선염(AEP)조、급성배사성이선염(ANP)조,각15지.술후0.5、6、16、24、48、72 h분비처사대서,취혈청검측C반응단백(CRP)、정분매수평,측이선조직함수량,행이선상규병리검사병평분,채용단백질인적법화면역조화법검측이선조직PIASI표체.기록대서72 h생존정황,행Cox다인소풍험평고.결과 ANP조16 h시적이선병리평분、이선함수량、혈청CRP화정분매수평분별위(7.70±2.55)분、(2.9l±0.57)％、(0.36 ±0.05) mg/L、(3141±625) U/L,균현저고우AEP조적(2.60±1.04)분、(2.04±0.47)％、(0.24±0.05) mg/L、(1984±637) U/L,역현저고우대조조적(0.80±0.42)분、(1.21±0.27)％、(0.14±0.03) mg/L、(978±353) U/L(P치＜0.05혹＜0.01).ANP조대서평균생존시간위(26.4±3.4)h,교AEP조적(57.3±4.2)h화대조조적(71.3±0.5)h현저축단(P치균＜0.01).ANP조16 h시적이선조직PIAS1단백표체교AEP조급대조조현저하조(0.10±0.01비0.80±0.07화0.87±0.05,P＜0.01).Cox분석현시PIAS1위영향AP대서생존시간적독립인소지일.결론 PIAS1재ANP중저표체,여AP병정엄중도정부상관,가이성위예측병정엄중정도급예후적지표.
Objective To investigate the expression of protein inhibitor of activated STAT-1 ( PIAS1 )in rat with acute pancreatitis (AP) and study its prediction value for severity and prognosis.Methods SD rats were randomly divided into control,acute edematous pancreatitis (AEP),and acute necrotizing pancreatitis (ANP) groups with 15 rats in each group.The rats were sacrificed at 0.5,6,16,24,48,72 h postoperatively.Serum CRP and amylase levels were determined.Water content of pancreas was measured.The pancreatic tissue was routinely harvested and underwent pathologic examination and was scored.The PIAS1protein expression was measured by Western blot and immunohistochemistry method.The survival rates at 72h were recorded and the risk analysis of multiple factors was investigated by Cóx regression method.Results The pathologic score,water content of pancreas,serum CRP and amylase levels at 16h in ANP group were (7.70 ±2.55),(2.91 ±0.57)％,(0.36 ±0.05)mg/L,(3141 ±625)U/L,which were significandy higher than those in AEP group [(2.60 ±1.04),(2.04 ±0.47)％,(0.24 ±0.05)mg/L,(1984 ± 637)U/L)],and also significantly higher than those in control group [ (0.80 ±0.42),( 1.21 ±0.27)％,(0.14 ±0.03)mg/L,(978 ± 353) U/L,(P ＜ 0.05or 0.01 ) ].The survival time of ANP rats was [ (26.36 ± 3.41 ) h,95％ CI:19.67～33.06 h],which were significantly shorter than that in AEP group [ (57.26 ±4.16)h,95％ CI:49.11 ～ 65.42) ],and also significantly shorter than that in control group [ (71.33 ±0.54) h,95％ CI:70.26 ～71.40,P ＜0.01 ].The expression of PIAS1 at 16h in ANP group was significantly lower than those in AEP group and control group ( 0.10 ± 0.01 vs.0.80 ± 0.07,0.87 ± 0.05,P ＜ 0.01 ).The Cox analysis suggested that PIAS1 was an independent prognosis factor for the survival time of AP rats.Conclusions PIAS1 is lowly to moderately expressed in ANP,and is negatively correlated with AP severity,and may be an independent risk factor for the prediction of severity and prognosis of AP.