中华生物医学工程杂志
中華生物醫學工程雜誌
중화생물의학공정잡지
CHINESE JOURNAL OF BIOMEDICAL ENGINEERING
2010年
2期
136-139
,共4页
金莉子%陈筱潮%马英东%陈剑%肖宏凯
金莉子%陳篠潮%馬英東%陳劍%肖宏凱
금리자%진소조%마영동%진검%초굉개
雌激素受体%冠心病%多因子遗传%多态性,单核苷酸%危险因素
雌激素受體%冠心病%多因子遺傳%多態性,單覈苷痠%危險因素
자격소수체%관심병%다인자유전%다태성,단핵감산%위험인소
Estrogen receptor%Coronary artery disease%Multifactorial inheritance%Polymorphism,Single nucleotide%Risk factor
目的 探讨中国人群中雌激素受体(ER)α基因Pvu Ⅱ和Xba Ⅰ酶切多态性与冠心病(CAD)的相关性.方法 将2004年4月至2006年12月在中山大学附属第五医院心内科住院的中国南方汉族CAD患者236例为病例组,117例选自健康体检者或同期在我院住院的非CAD患者为对照组,应用聚合酶链反应-限制片断长度多态性(PCR-RFLP)分析的方法,检测CAD组和对照组的ERα基因型,比较其与相关指标的关系.结果 ERα酶切多态性分析结果显示Pvu Ⅱ存在PP、Pp、pp 3种基因型;Xba Ⅰ酶切也可区分出XX、Xx、xx 3型.Pvu Ⅱ多态性中,CAD组P等位基因型频率也显著高于对照组[42.2%(199/472)比33.8%(79/234),P=0.032],pp基因型的高密度脂蛋白水平显著高于P等位基因携带者,两组之间基因型分布差异具有统计学意义(P=0.041),X等位基因在对照组和CAD组分别为16.5%(78/472)和16.2%(38/234),两组基因型和等位基因频率差异均无统计学意义.结论 中国南方汉族人群中ERαPvu Ⅱ酶切多态性与CAD有关,P等位基因可能是CAD独立遗传危险因素;ERα Xba Ⅰ酶切多态性与CAD未发现相关.
目的 探討中國人群中雌激素受體(ER)α基因Pvu Ⅱ和Xba Ⅰ酶切多態性與冠心病(CAD)的相關性.方法 將2004年4月至2006年12月在中山大學附屬第五醫院心內科住院的中國南方漢族CAD患者236例為病例組,117例選自健康體檢者或同期在我院住院的非CAD患者為對照組,應用聚閤酶鏈反應-限製片斷長度多態性(PCR-RFLP)分析的方法,檢測CAD組和對照組的ERα基因型,比較其與相關指標的關繫.結果 ERα酶切多態性分析結果顯示Pvu Ⅱ存在PP、Pp、pp 3種基因型;Xba Ⅰ酶切也可區分齣XX、Xx、xx 3型.Pvu Ⅱ多態性中,CAD組P等位基因型頻率也顯著高于對照組[42.2%(199/472)比33.8%(79/234),P=0.032],pp基因型的高密度脂蛋白水平顯著高于P等位基因攜帶者,兩組之間基因型分佈差異具有統計學意義(P=0.041),X等位基因在對照組和CAD組分彆為16.5%(78/472)和16.2%(38/234),兩組基因型和等位基因頻率差異均無統計學意義.結論 中國南方漢族人群中ERαPvu Ⅱ酶切多態性與CAD有關,P等位基因可能是CAD獨立遺傳危險因素;ERα Xba Ⅰ酶切多態性與CAD未髮現相關.
목적 탐토중국인군중자격소수체(ER)α기인Pvu Ⅱ화Xba Ⅰ매절다태성여관심병(CAD)적상관성.방법 장2004년4월지2006년12월재중산대학부속제오의원심내과주원적중국남방한족CAD환자236례위병례조,117례선자건강체검자혹동기재아원주원적비CAD환자위대조조,응용취합매련반응-한제편단장도다태성(PCR-RFLP)분석적방법,검측CAD조화대조조적ERα기인형,비교기여상관지표적관계.결과 ERα매절다태성분석결과현시Pvu Ⅱ존재PP、Pp、pp 3충기인형;Xba Ⅰ매절야가구분출XX、Xx、xx 3형.Pvu Ⅱ다태성중,CAD조P등위기인형빈솔야현저고우대조조[42.2%(199/472)비33.8%(79/234),P=0.032],pp기인형적고밀도지단백수평현저고우P등위기인휴대자,량조지간기인형분포차이구유통계학의의(P=0.041),X등위기인재대조조화CAD조분별위16.5%(78/472)화16.2%(38/234),량조기인형화등위기인빈솔차이균무통계학의의.결론 중국남방한족인군중ERαPvu Ⅱ매절다태성여CAD유관,P등위기인가능시CAD독립유전위험인소;ERα Xba Ⅰ매절다태성여CAD미발현상관.
Objective To elucidate the association of estrogen receptor α gene (ERα)Puv Ⅱ and Xba Ⅰ polymorphisms and susceptibility to coronary artery disease (CAD). Methods The subjects were Hans population in southern China,consisting of 236 documented Han patients with CAD treated in the Department of Cardiology,the Fifth Affiliated Hospital of Zhongshan University between April 2004 and December 2006,as the study group,and 117 apparently healthy individuals or contemporary non-CAD patients as the control group. Polymerase chain reaction and restriction fragment length polymorphisms (PCR-RFLP) were used to determine the genotypes of ERα as related to blood lipid level. Results Digested polymorphism analysis showed PP,Pp and pp as genotypes of Pvu Ⅱ,and XX,Xx and xx as genotypes of Xba Ⅰ. The frequency of P allele at Puv Ⅱ site was significantly higher in CAD group than that in the healthy control group[42.2% (199/472) vs 33.8% (79/234),P=0.032]. Subjects with pp genotype showed higher level of high-density lipoprotein as compared with P allele carriers. There was a significant difference in the distribution of Pvu Ⅱ genotypes between two groups (P=0.041). The frequency of X allele was 16.5% (78/472) in the healthy control group vs 16.2% (38/234) in the CAD group. No differences in genotype distribution and allele frequency were found between two groups at Xba Ⅰ site. Conclusions ERα Pvu Ⅱ but not Xba Ⅰ polymorphisms in southern China Hans may be associated with the susceptibility to CAD. P allele may be a genetic risk factor of CAD in this population.
