中华胃肠外科杂志
中華胃腸外科雜誌
중화위장외과잡지
CHINESE JOURNAL OF GASTROINTESTINAL SURGERY
2011年
5期
364-367
,共4页
孙家磊%朱宝松%龚巍%张鹏%郁立衍%赵奎%邢春根
孫傢磊%硃寶鬆%龔巍%張鵬%鬱立衍%趙奎%邢春根
손가뢰%주보송%공외%장붕%욱립연%조규%형춘근
磷脂酰肌醇3-激酶%NF-κB P65%胃肿瘤%细胞凋亡
燐脂酰肌醇3-激酶%NF-κB P65%胃腫瘤%細胞凋亡
린지선기순3-격매%NF-κB P65%위종류%세포조망
Phosphatidylinositol-3-kinases%NF-κB P65%Stomach neoplasms%Cell apoptosis
目的 探讨磷脂酰肌醇3-激酶(PI3K)抑制剂LY294002与NF-κB P65核转运抑制剂SN50联合应用对裸鼠荷人胃癌模型的影响.方法 采用裸鼠皮下胃癌SGC7901细胞注射法建立移植瘤模型,将模型鼠分为对照组、LY294002干预组、SN50干预组、LY294002与SN50联合干预组,每组5只.干预处理10 d后观察各组肿瘤大小并计算每组肿瘤抑制率.用免疫组织化学法检测Bcl-2、P53、Bax蛋白表达,并用透射电子显微镜从形态变化上观察各组肿瘤细胞凋亡情况.结果 药物干预10 d后,联合干预组裸鼠胃癌细胞生长抑制率为(49.2±2.5)%,明显高于LY294002干预组(29.4±1.5)%和SN50干预组(19.7±1.6)%,差异有统计学意义(P<0.05).此外,与LY294002干预组和SN50干预组相比,联合干预组Bcl-2表达下调和P53、Bax表达上调更为显著(P<0.05),胃癌细胞凋亡程度更为严重.结论 LY294002与SN50联合应用可以显著抑制人胃癌SGC7901细胞裸鼠移植瘤的生长并促进胃癌细胞凋亡.
目的 探討燐脂酰肌醇3-激酶(PI3K)抑製劑LY294002與NF-κB P65覈轉運抑製劑SN50聯閤應用對裸鼠荷人胃癌模型的影響.方法 採用裸鼠皮下胃癌SGC7901細胞註射法建立移植瘤模型,將模型鼠分為對照組、LY294002榦預組、SN50榦預組、LY294002與SN50聯閤榦預組,每組5隻.榦預處理10 d後觀察各組腫瘤大小併計算每組腫瘤抑製率.用免疫組織化學法檢測Bcl-2、P53、Bax蛋白錶達,併用透射電子顯微鏡從形態變化上觀察各組腫瘤細胞凋亡情況.結果 藥物榦預10 d後,聯閤榦預組裸鼠胃癌細胞生長抑製率為(49.2±2.5)%,明顯高于LY294002榦預組(29.4±1.5)%和SN50榦預組(19.7±1.6)%,差異有統計學意義(P<0.05).此外,與LY294002榦預組和SN50榦預組相比,聯閤榦預組Bcl-2錶達下調和P53、Bax錶達上調更為顯著(P<0.05),胃癌細胞凋亡程度更為嚴重.結論 LY294002與SN50聯閤應用可以顯著抑製人胃癌SGC7901細胞裸鼠移植瘤的生長併促進胃癌細胞凋亡.
목적 탐토린지선기순3-격매(PI3K)억제제LY294002여NF-κB P65핵전운억제제SN50연합응용대라서하인위암모형적영향.방법 채용라서피하위암SGC7901세포주사법건립이식류모형,장모형서분위대조조、LY294002간예조、SN50간예조、LY294002여SN50연합간예조,매조5지.간예처리10 d후관찰각조종류대소병계산매조종류억제솔.용면역조직화학법검측Bcl-2、P53、Bax단백표체,병용투사전자현미경종형태변화상관찰각조종류세포조망정황.결과 약물간예10 d후,연합간예조라서위암세포생장억제솔위(49.2±2.5)%,명현고우LY294002간예조(29.4±1.5)%화SN50간예조(19.7±1.6)%,차이유통계학의의(P<0.05).차외,여LY294002간예조화SN50간예조상비,연합간예조Bcl-2표체하조화P53、Bax표체상조경위현저(P<0.05),위암세포조망정도경위엄중.결론 LY294002여SN50연합응용가이현저억제인위암SGC7901세포라서이식류적생장병촉진위암세포조망.
Objective To investigate the effect of phosphatidylinositol 3 -kinase inhibitor LY294002 combined with NF-κB P65 nuclear translocation inhibitor SN50 on the tumor cell growth and apoptosis using a nude mouse model of gastric cancer. Methods Human gastric cancer cell strain SGC7901 was transplanted subcutaneously to nude mice to establish tumor models. Model mice were randomly divided into the control group, the LY294002 treatment group, the SN50 treatment group,and the LY294002+SN50 treatment group, with 5 in each group. After being treated for 10 days, the inhibition rate of tumor growth was ascertained by measuring the size of tumor. Immunohistochemical method was used to detect the expression levels of Bcl-2, P53 and Bax proteins and transmission electron microscopy to investigate the apoptosis of tumor cells. Results On the 10th day after treatment, the inhibition rate of gastric cancer cellular growth in the LY294002+SN50 group was (49.2±2.5)%, which was significantly higher than that in the LY294002 group (29.4±1.5)% and SN50 group (19.7 ±1.6)% (P<0.05). In comparison with the other two groups, LY294002+SN50 group exhibited more severe apoptosis, with expression of Bcl-2 decreased and that of P53 and Bax increased more significantly (P<0.05). Conclusion LY294002 combined with SN50 inhibits the growth of SGC7901 transplanted tumor and aggravates the apoptosis of gastric cancer cells in nude mice model.
