CAJ | 학술논문

目的探讨转录抑制因子Snail表达与胃癌Lauren分型的关系.方法 Western印迹检测肠型胃癌细胞系(N87)和弥漫型胃癌细胞系(AGS)中Snail和上皮型E钙黏蛋白(E-cadherin)的表达.将糖原合酶激酶-3β(GSK-3β)质粒转染胃癌细胞系,检测Snail和E-cadherin的表达变化.收集2000年2月至2005年12月间在复旦大学附属中山医院行胃癌根治术的77例术后组织标本,采用免疫组织化学染色检测Snail在胃癌组织中表达,并分析其与胃癌Lauren分型之间的关系.结果 Snail在N87中低表达,在AGS中高表达；E-cadherin表达与Snail相反.转染GSK-3β后,胃癌细胞Snail表达显著下调,E-cadherin表达显著上调(P＜0.01).使用不同浓度的GSK-3β抑制剂氯化锂处理后,胃癌细胞Snail表达显著上调,且具有明显浓度依赖性(P＜0.01).21例肠型胃癌中Snail低表达16例,高表达5例；56例弥漫型胃癌中Snail低表达21例,高表达35例；肠型胃癌中Snail表达明显弱于弥漫型,差异有统计学意义(P＜0.01).结论 Snail的表达与胃癌Lauren分型有关,是一种潜在的确定胃癌分型的分子标志物.
목적 탐토전록억제인자Snail표체여위암Lauren분형적관계.방법 Western인적검측장형위암세포계(N87)화미만형위암세포계(AGS)중Snail화상피형E개점단백(E-cadherin)적표체.장당원합매격매-3β(GSK-3β)질립전염위암세포계,검측Snail화E-cadherin적표체변화.수집2000년2월지2005년12월간재복단대학부속중산의원행위암근치술적77례술후조직표본,채용면역조직화학염색검측Snail재위암조직중표체,병분석기여위암Lauren분형지간적관계.결과 Snail재N87중저표체,재AGS중고표체；E-cadherin표체여Snail상반.전염GSK-3β후,위암세포Snail표체현저하조,E-cadherin표체현저상조(P＜0.01).사용불동농도적GSK-3β억제제록화리처리후,위암세포Snail표체현저상조,차구유명현농도의뢰성(P＜0.01).21례장형위암중Snail저표체16례,고표체5례；56례미만형위암중Snail저표체21례,고표체35례；장형위암중Snail표체명현약우미만형,차이유통계학의의(P＜0.01).결론 Snail적표체여위암Lauren분형유관,시일충잠재적학정위암분형적분자표지물.
Objective To explore the association of Snail expression and Lauren classification of gastric cancer.Methods The protein levels of Snail and E-cadherin were detected by Western blot in N87(intestinal-type gastric cancer cell line) and AGS(diffuse-type gastric cancer cell line) cell lines and those after transfection of GSK-3β plasmid.The study included a total of 77 patients with primary gastric cancer who underwent curative gastrectomy in the Zhongshan Hospital from February 2000 to December 2005 without any chemotherapy or radiation therapy before surgery.Tissues of gastric cancer specimens were stained using immunohistochemistry to determine Snail expression.Results Snail expression was low in N78 and high in AGS.E-cadherin expression showed reverse expression pattern.After transfection with GSK-3β,the expression of Snail was significantly suppressed and that of E-cadherinelevated (P＜0.01).Different concentrations of GSK-33 inhibitor lithion chloride were used to treat the cell lines and Snail expression was significantly up-regulated in a dose-dependent manner (P＜0.01).Snail expression was elevated in 16 out of 21 N78 cell lines,and in 21 out of 56 AGS cell lines,and the difference was statistically significant (P＜0.01).Conclusion The expression of Snail is closely associated with the Lauren classification of gastric cancer,and it may be a potential marker of the gastric cancer classification.