CAJ | 학술논문

目的探讨不同营养方式对肝硬化大鼠肝切除术后肝脏自然杀伤(NK)细胞数量和活性的影响。方法采用硫代乙酰胺诱导大鼠肝硬化模型。将20只肝硬化大鼠按随机号码表法随机分为肠内营养组和肠外营养组，每组10只，分别于肝切除术后1d开始进行为期5d的肠内营养和肠外营养。应用流式细胞术测定大鼠肝脏NK细胞百分率；4 h51Cr释放法测定肝脏NK细胞的杀伤活性。结果肠内营养组的肝脏NK细胞百分率（22.2％±1.7％比17.4％±1.8％，P=0.000）和NK细胞杀伤活性均明显高于肠外营养组（41.6％±2.3％比37.1％±2.2％，P=0.000）。结论肠内营养可以增加肝硬化大鼠肝切除术后肝脏NK细胞的百分率和杀伤活性。
목적 탐토불동영양방식대간경화대서간절제술후간장자연살상(NK)세포수량화활성적영향。방법 채용류대을선알유도대서간경화모형。장20지간경화대서안수궤호마표법수궤분위장내영양조화장외영양조，매조10지，분별우간절제술후1d개시진행위기5d적장내영양화장외영양。응용류식세포술측정대서간장NK세포백분솔；4 h51Cr석방법측정간장NK세포적살상활성。결과 장내영양조적간장NK세포백분솔（22.2％±1.7％비17.4％±1.8％，P=0.000）화NK세포살상활성균명현고우장외영양조（41.6％±2.3％비37.1％±2.2％，P=0.000）。결론 장내영양가이증가간경화대서간절제술후간장NK세포적백분솔화살상활성。
Objective To explore the effects of different nutrition methods on the number and activity of natural killer (NK) cells in liver after hepatectomy in cirrhotic rats. Methods Twenty thioacetamide-induced cirrhotic rats were randomly divided into enteral nutrition (EN) group ( n =10) and parenteral nutrition (PN) group ( n =10), in which EN or PN was administered for 5 consecutive days 1 day after hepatectomy. The percentage of the NK cells in livers was examined by flow cytometry. Activity of NK cells was measured by 4 h51Cr-release assay.Results Compared with the PN group, the percentage (22.2 ％ ± 1.7％ vs. 17.4％ ± 1.8％, P =0.000) and the activity of NK cells (41.6％ ± 2.3％ vs. 37.1％ ± 2.2％, P =0.000) in the livers were significantly higher in the EN group. Conclusion EN after posthepatectomy can ameliorate the percentage and the activity of NK cells in the livers in cirrhotic rats.