CAJ | 학술논문

目的观察滨蒿内酯对哮喘大鼠气道壁平滑肌增殖及α-平滑肌肌动蛋白(α-SMA)表达的影响,探讨滨蒿内酯治疗哮喘的作用机制.方法 40只雄性大鼠随机分成4组:正常对照组(N组)、哮喘组(A组)、地塞米松治疗组(D组)及滨蒿内酯治疗组(S组),以卵清蛋白致敏和激发方法建立哮喘大鼠动物模型并给予相应治疗,光镜下测定平滑肌层厚度及内外径,免疫组化法检测α-耳滑肌肌动蛋白(α-SMA)在肺组织的表达.结果哮喘模型组大鼠表现为呼气时间延长,呼气峰压明显增大,模型复制成功;A组气道壁α-SMA蛋白表达阳性率和平滑肌层厚度较N组均显著增加(均为P＜0.01),气道内外径比值较正常组减小(P＜0.01),S组α-SMA蛋白表达及平滑肌层厚度较A组减少,两两比较差异有统计学意义(P＜0.01).结论滨蒿内酯能够抑制α-SMA的表达,减轻气道炎症反应、减轻平滑肌增殖,延缓气道重构进程.
목적 관찰빈호내지대효천대서기도벽평활기증식급α-평활기기동단백(α-SMA)표체적영향,탐토빈호내지치료효천적작용궤제.방법 40지웅성대서수궤분성4조:정상대조조(N조)、효천조(A조)、지새미송치료조(D조)급빈호내지치료조(S조),이란청단백치민화격발방법건립효천대서동물모형병급여상응치료,광경하측정평활기층후도급내외경,면역조화법검측α-이활기기동단백(α-SMA)재폐조직적표체.결과 효천모형조대서표현위호기시간연장,호기봉압명현증대,모형복제성공;A조기도벽α-SMA단백표체양성솔화평활기층후도교N조균현저증가(균위P＜0.01),기도내외경비치교정상조감소(P＜0.01),S조α-SMA단백표체급평활기층후도교A조감소,량량비교차이유통계학의의(P＜0.01).결론 빈호내지능구억제α-SMA적표체,감경기도염증반응、감경평활기증식,연완기도중구진정.
Objective To investigate the influence of curcumine on α-smooth actiu(SMA) expression,and to explore the role of curcumine in dealing with asthmatic rats.Methods 40 SD rats were randomly divided into 4 groups:normal group,model group,dexamethasone group,scoparone group.The asthmatic rats model was established by repeated inhalation of ovalbulium.The pathological structure were detected by HE staining,and α-SMA expression in pulmonary tissues were detectd by immunohistochemistry.The changes of α-SMA content in the wall of airway and the thickness of airway smooth muscle layer,inner and outer diameter were measured by the computerized image analysis system.Results After challenged,the changes of pulmonary function were accompanied with prolonged expiratory time and elevated expiratory pressure.The expression of α-SMA,the thickness of airway smooth muscle layer were significantly higher(P＜0.01) in the model group than that in the normal group,while the ratio of inner diameter to outer diameter were lower(P＜0.01),There were statistical significance between group (P＜0.01).Conclusion Scoparone could inhibit the expression of α-SMA,relieve airway inflammation and smooth muscle proliferation,delay the process of airway remodelding.