CAJ | 학술논문

目的研究膀胱尿路上皮癌组织中Snail蛋白表达与E-cadherin蛋白、T细胞亚群的相关性。方法采用免疫组化SP法检测156例膀胱尿路上皮癌组织和80例癌旁组织中Snail蛋白、E-cadherin蛋白的表达情况，比较二者在不同病理组织中的阳性表达率，并分析二者表达的相关性；分析膀胱尿路上皮癌组织中Snail蛋白阳性表达和CD4+、CD8+细胞数量及CD4+/CD8+的相关性。结果膀胱尿路上皮癌组织Snail蛋白阳性表达率65.4％(102/156)显著高于癌旁组织的48.8％(39/80)，差异有统计学意义(P＜0.05)；其表达与膀胱尿路上皮癌的临床分期、病理分级、肿瘤数量、远处转移及复发有关（P值均＜0.05）。膀胱尿路上皮癌中Snail蛋白与E-cadherin蛋白表达呈负相关（r= -0.186，P＜0.05）；Snail蛋白阳性表达与CD4+细胞数量以及CD4+/CDt+值呈负相关(r=-0.313，P＜0.05；r=-0.305，P＜0.05)，而与CDs+细胞数量无关（r= -0.250，P＞0.05）。结论 Snail蛋白可能通过抑制E-cadherin蛋白表达及诱导膀胱肿瘤局部免疫抑制，促进膀胱尿路上皮癌的浸润、转移。
목적 연구방광뇨로상피암조직중Snail단백표체여E-cadherin단백、T세포아군적상관성。 방법 채용면역조화SP법검측156례방광뇨로상피암조직화80례암방조직중Snail단백、E-cadherin단백적표체정황，비교이자재불동병리조직중적양성표체솔，병분석이자표체적상관성；분석방광뇨로상피암조직중Snail단백양성표체화CD4+、CD8+세포수량급CD4+/CD8+적상관성。결과방광뇨로상피암조직Snail단백양성표체솔65.4％(102/156)현저고우암방조직적48.8％(39/80)，차이유통계학의의(P＜0.05)；기표체여방광뇨로상피암적림상분기、병리분급、종류수량、원처전이급복발유관（P치균＜0.05）。방광뇨로상피암중Snail단백여E-cadherin단백표체정부상관（r= -0.186，P＜0.05）；Snail단백양성표체여CD4+세포수량이급CD4+/CDt+치정부상관(r=-0.313，P＜0.05；r=-0.305，P＜0.05)，이여CDs+세포수량무관（r= -0.250，P＞0.05）。 결론 Snail단백가능통과억제E-cadherin단백표체급유도방광종류국부면역억제，촉진방광뇨로상피암적침윤、전이。
Objective To investigate the expression of Snail in bladder urothelial carcinoma and evaluate its relationship with E-cadherin and a subset of T cell groups.Methods Immunohistochemical method was used to detect the expression of Snail and E-cadherin proteins in tissue from 156 cases of bladder urothelial carcinoma and 80 cases of the para-cancerous mucosa. The proteins expression status was analyzed with clinico-pathological data. Meanwhile, correlations with Snail and CD4+, CD8+, CD4+/CD8+ were analyzed.Results The positive rate of Snail in bladder urothelial carcinoma was 65.4％ , which was significantly higher than that in para-cancerous mucosa (48.8％). The expression of Snail was significantly correlated with the clinical stage, pathological grade, tumor quantity, distant metastasis and tumor recurrence.There was negative correlation between the expression of Snail and E-cadherin in bladder urothelial carcinoma. Positive expression of Snail had a negative correlation with the number of CD4+ and CD4+/CD8+ , whereas it had no significant correlation with the number of CD8+.Conclusions By inhibition of the E-cadherin expression and inducing local immunosuppression, Snail might play an important role in the process of the invasion and metastasis of bladder urothelial carcinoma.