中华心血管病杂志
中華心血管病雜誌
중화심혈관병잡지
Chinese Journal of Cardiology
2012年
1期
3-7
,共5页
孙倩%李清贤%宋喜发%郑松责%晏峰%陈鹏%唐疾飞%牛宇欣%包其郁%张国强%胡云良
孫倩%李清賢%宋喜髮%鄭鬆責%晏峰%陳鵬%唐疾飛%牛宇訢%包其鬱%張國彊%鬍雲良
손천%리청현%송희발%정송책%안봉%진붕%당질비%우우흔%포기욱%장국강%호운량
高血压%多态性,单核苷酸%钙通道阻滞药
高血壓%多態性,單覈苷痠%鈣通道阻滯藥
고혈압%다태성,단핵감산%개통도조체약
Hypertension%Polymorphism,single nucleotide%Calcium channel blockers
目的 探讨L型钙离子通道 alc 哑基基因(CACNAlC)多态性对钙离子通道阻滞剂治疗原发性高血压(EH)患者疗效的影响.方法 对103例EH患者,给单一硝苯地平缓释片进行6周的治疗,采用多重聚合酶链反应方法(Multi-PCR)及基质辅助激光解析-飞行时间质谱分析技术(MLDI-TOF MS)对 rs216008、rs1051375、rs2299661、rs10848683、rs215976进行分型,分析不同基因型间治疗前后血压变化的差值.结果(1)与治疗前相比,硝苯地平缓释片治疗后所有患者平均收缩压和舒张压均出现明显下降(P＜0.05).(2)rs2299661的CC型舒张压下降幅度明显高于CG型和GG型[(12.46±7.91)mm Hg(1 mm Hg =0.133 kPa)比(7.22±8.01)mm Hg和(5.93±9.77)mm Hg,P＜0.05].(3)rs216008的CC型收缩压下降幅度明显高于CT型[(20.60±12.35)mm Hg比(13.62±10.21)mm Hg,P＜0.05],但与TT型比较差异无统计学意义.(4)rs1051375、rs10848683、rs215976的各基因型间血压变化差值无统计学意义.结论 CACNAI C rs2299661、rs216008的CC型可能会使硝苯地平缓释片治疗EH患者的疗效具更为突出.
目的 探討L型鈣離子通道 alc 啞基基因(CACNAlC)多態性對鈣離子通道阻滯劑治療原髮性高血壓(EH)患者療效的影響.方法 對103例EH患者,給單一硝苯地平緩釋片進行6週的治療,採用多重聚閤酶鏈反應方法(Multi-PCR)及基質輔助激光解析-飛行時間質譜分析技術(MLDI-TOF MS)對 rs216008、rs1051375、rs2299661、rs10848683、rs215976進行分型,分析不同基因型間治療前後血壓變化的差值.結果(1)與治療前相比,硝苯地平緩釋片治療後所有患者平均收縮壓和舒張壓均齣現明顯下降(P＜0.05).(2)rs2299661的CC型舒張壓下降幅度明顯高于CG型和GG型[(12.46±7.91)mm Hg(1 mm Hg =0.133 kPa)比(7.22±8.01)mm Hg和(5.93±9.77)mm Hg,P＜0.05].(3)rs216008的CC型收縮壓下降幅度明顯高于CT型[(20.60±12.35)mm Hg比(13.62±10.21)mm Hg,P＜0.05],但與TT型比較差異無統計學意義.(4)rs1051375、rs10848683、rs215976的各基因型間血壓變化差值無統計學意義.結論 CACNAI C rs2299661、rs216008的CC型可能會使硝苯地平緩釋片治療EH患者的療效具更為突齣.
목적 탐토L형개리자통도 alc 아기기인(CACNAlC)다태성대개리자통도조체제치료원발성고혈압(EH)환자료효적영향.방법 대103례EH환자,급단일초분지평완석편진행6주적치료,채용다중취합매련반응방법(Multi-PCR)급기질보조격광해석-비행시간질보분석기술(MLDI-TOF MS)대 rs216008、rs1051375、rs2299661、rs10848683、rs215976진행분형,분석불동기인형간치료전후혈압변화적차치.결과(1)여치료전상비,초분지평완석편치료후소유환자평균수축압화서장압균출현명현하강(P＜0.05).(2)rs2299661적CC형서장압하강폭도명현고우CG형화GG형[(12.46±7.91)mm Hg(1 mm Hg =0.133 kPa)비(7.22±8.01)mm Hg화(5.93±9.77)mm Hg,P＜0.05].(3)rs216008적CC형수축압하강폭도명현고우CT형[(20.60±12.35)mm Hg비(13.62±10.21)mm Hg,P＜0.05],단여TT형비교차이무통계학의의.(4)rs1051375、rs10848683、rs215976적각기인형간혈압변화차치무통계학의의.결론 CACNAI C rs2299661、rs216008적CC형가능회사초분지평완석편치료EH환자적료효구경위돌출.
Objective To explore the relationship between genetic polymorphisms of CACNA1C that encoded the alc subunit of the L-type calcium channel and the efficacy of calcium channel blocker (CCB,Nifedipine extended release tablet/20 mg/d)in essential hypertension(EH)patients of Han Chinese in Wenzhou.Methods For the enrolled 103 EH patients,Multiplex Polymerase Chain Reaction(Muhi PCR)and matrix assisted laser desorption ionization time of flight MS(MLD1-TOF MS)were performed to detect their genotypes(rs216008,rs1051375,rs2299661,rs10848683,rs215976),blood pressure(BP)after CCB monotherapy was compared among patients with different genotypes.Results(1)Blood pressure was significantly reduced in all patients post CCB(P ＜ 0.05 vs.pre-CCB).(2)Diastolic blood pressure reduction was more significant in subjects with rs2299661 C/C genotype(wild genotype)than in subjects with rs2299661C/G and rs2299661G/G genotype(mutational genotype)[(12.46 ± 7.91)mm Hg (1 mm Hg=0.133 kPa)vs.(7.22±8.01)mm Hgand(5.93 ± 9.77)mm Hg,P＜0.05].(3)Systolic blood pressure reduction was more significant in subjects with rs216008 C/C genotype(wild genotype)than in subjects with rs216008 C/T genotype(mutational genotype)[(20.60 ± 12.35)mm Hg vs.(13.62 ±10.21)mm Hg,P ＜0.05].(4)Blood pressure reduction was similar between subjects with genotype of rs1051375,rs10848683 and rs215976.Conclusion EH patients with wild genotype of rs2299661 and rs216008 in CACNA1 C are more likely to be responders of CCB monotherapy.
