分析试验室
分析試驗室
분석시험실
ANALYTICAL LABORATORY
2010年
1期
1-4
,共4页
鄂红军%王思明%苏萍%杨屹
鄂紅軍%王思明%囌萍%楊屹
악홍군%왕사명%소평%양흘
毛细管区带电泳%毛细管酶微反应器%N-乙酰-DL-蛋氨酸
毛細管區帶電泳%毛細管酶微反應器%N-乙酰-DL-蛋氨痠
모세관구대전영%모세관매미반응기%N-을선-DL-단안산
Capillary zone electrophoresis%Capillary enzyme micro-reactor%N-acetyl-DL-methionine
将氨基酰化酶通过戊二醛固定在毛细管内壁, 制备毛细管酶微反应器, 用毛细管区带电泳对毛细管酶微反应器的酶解产物进行分离, 以生成物的峰面积优化底物N-乙酰-DL-蛋氨酸的酶解条件. 实验结果表明, 在温度37 ℃的条件下, 10 μg/mL N-乙酰-DL-蛋氨酸磷酸盐缓冲溶液(pH 7.5)以4 μL/min的速度通过15 cm长的毛细管酶微反应器, 具有良好的酶解效果. 利用毛细管酶微反应器对底物N-乙酰-DL-蛋氨酸进行酶解, 每天酶解5次, 10天后酶活仅下降了8.66%, 说明制备的毛细管酶微反应器具有良好的稳定性.
將氨基酰化酶通過戊二醛固定在毛細管內壁, 製備毛細管酶微反應器, 用毛細管區帶電泳對毛細管酶微反應器的酶解產物進行分離, 以生成物的峰麵積優化底物N-乙酰-DL-蛋氨痠的酶解條件. 實驗結果錶明, 在溫度37 ℃的條件下, 10 μg/mL N-乙酰-DL-蛋氨痠燐痠鹽緩遲溶液(pH 7.5)以4 μL/min的速度通過15 cm長的毛細管酶微反應器, 具有良好的酶解效果. 利用毛細管酶微反應器對底物N-乙酰-DL-蛋氨痠進行酶解, 每天酶解5次, 10天後酶活僅下降瞭8.66%, 說明製備的毛細管酶微反應器具有良好的穩定性.
장안기선화매통과무이철고정재모세관내벽, 제비모세관매미반응기, 용모세관구대전영대모세관매미반응기적매해산물진행분리, 이생성물적봉면적우화저물N-을선-DL-단안산적매해조건. 실험결과표명, 재온도37 ℃적조건하, 10 μg/mL N-을선-DL-단안산린산염완충용액(pH 7.5)이4 μL/min적속도통과15 cm장적모세관매미반응기, 구유량호적매해효과. 이용모세관매미반응기대저물N-을선-DL-단안산진행매해, 매천매해5차, 10천후매활부하강료8.66%, 설명제비적모세관매미반응기구유량호적은정성.
The capillary enzyme micro-reactor was prepared by immobilizing aminoacylase on the inner wall of capillary with glutaraldehyde as a cross-linking agent. The enzymatic hydrolysis of N-acetyl-DL-methioine in the prepared micro-reactor was evaluated by capillary zone electrophoresis. The results showed that high efficiency of the enzymatic hydrolysis could be obtained at pH 7.5 and 37 ℃ for 10 μg/mL N-acetyl-DL-methioine with a flow rate of 4 μL/min in a 15 cm capillary enzyme micro-reactor. The enzyme micro-reactor was stable while its activity decreased less than 8.66% within 10 days.
