中国医学科学院学报
中國醫學科學院學報
중국의학과학원학보
ACTA ACADEMIAE MEDICINAE SINICAE
2009年
6期
724-727
,共4页
SirT7%细胞增殖%细胞周期
SirT7%細胞增殖%細胞週期
SirT7%세포증식%세포주기
SirT7%proliferation%cell cycle
目的 构建稳定表达SirT7的P19细胞系并探讨Ⅲ类组蛋白去乙酰基酶SirT7在该细胞中对细胞增殖和细胞周期的影响.方法 将SirT7的真核表达质粒转染进入P19细胞,并利用筛选标记筛选出稳定克隆,采用Western blot、流式细胞仪等方法观察SirT7对P19细胞生长和细胞周期的影响.结果 稳定表达Sirt7 P19 细胞系细胞生长速度减慢,流式细胞仪荧光分析呈现明显的G1/S期阻滞. 结论SirT7可导致P19细胞的周期阻滞,并抑制细胞的增殖.
目的 構建穩定錶達SirT7的P19細胞繫併探討Ⅲ類組蛋白去乙酰基酶SirT7在該細胞中對細胞增殖和細胞週期的影響.方法 將SirT7的真覈錶達質粒轉染進入P19細胞,併利用篩選標記篩選齣穩定剋隆,採用Western blot、流式細胞儀等方法觀察SirT7對P19細胞生長和細胞週期的影響.結果 穩定錶達Sirt7 P19 細胞繫細胞生長速度減慢,流式細胞儀熒光分析呈現明顯的G1/S期阻滯. 結論SirT7可導緻P19細胞的週期阻滯,併抑製細胞的增殖.
목적 구건은정표체SirT7적P19세포계병탐토Ⅲ류조단백거을선기매SirT7재해세포중대세포증식화세포주기적영향.방법 장SirT7적진핵표체질립전염진입P19세포,병이용사선표기사선출은정극륭,채용Western blot、류식세포의등방법관찰SirT7대P19세포생장화세포주기적영향.결과 은정표체Sirt7 P19 세포계세포생장속도감만,류식세포의형광분석정현명현적G1/S기조체. 결론SirT7가도치P19세포적주기조체,병억제세포적증식.
Objective To study the regulatory role of SirT7, one of class Ⅲ histone deacetylases, on the proliferation of mouse embryonal carcinoma cell line P19. MethodsWe used an expression plasmid of SirT7 (151-402 amino acid residues) and its vector respectively to establish a stably expressed SirT7 and its control P19 cell lines. Recombinant DNA techniques, Western blot, cell growth curve, and flow cytometry were used in this paper. ResultsCompared with the control cells, the P19 cells had significantly lower growth rate in stably expressed SirT7. G1 to S cell cycle arrests were only seen in the SirT7 over-expressed cell line. ConclusionSirT7 play a dominant role in the grow inhibition of the P19 cells.
