CAJ | 학술논문

目的:用乙醇注入法制备紫草素脂质体,使用正交设计并结合星点设计/效应面法优选最佳处方.方法:以紫草素与脂质比,豆磷脂与胆固醇比,乙醇量与水量比,豆磷脂浓度和甘露醇浓度5个影响因子进行正交实验;进一步采用星点设计优化处方,实验结果进行多无线性和二项式、三项式非线性方程拟合,效应面法确定最佳处方配比.结果:三次多项式是描述各指标与因素之间的最佳模型.优化处方制备脂质体的包封率为85.4%、载药量为5.16%,理论预测值与实测值偏差较小,模型具有良好的预测性.结论:正交设计联用星点设计-效应面优化法筛选脂质体处方是一种简便、可行的实验设计方法.
목적:용을순주입법제비자초소지질체,사용정교설계병결합성점설계/효응면법우선최가처방.방법:이자초소여지질비,두린지여담고순비,을순량여수량비,두린지농도화감로순농도5개영향인자진행정교실험;진일보채용성점설계우화처방,실험결과진행다무선성화이항식、삼항식비선성방정의합,효응면법학정최가처방배비.결과:삼차다항식시묘술각지표여인소지간적최가모형.우화처방제비지질체적포봉솔위85.4%、재약량위5.16%,이론예측치여실측치편차교소,모형구유량호적예측성.결론:정교설계련용성점설계-효응면우화법사선지질체처방시일충간편、가행적실험설계방법.
AIM:To select an optiomal formulation of liposome entrapped alkanin prepared with ethanol injection in the combination of orthogonal design and central composite design/Response surface method.METHODS:Five influencing factors,including ratio of alkanin to liposome and soyabean lecithin to cholesterol,volume ratio of ethanol phase to water phase,soybean concentration and mannitol concentration were chosen as indices by orthogonal design.Then the method utilized the central composite design,which fitted multivariate linear and second/third order nonlinear regression equation,to find out the optimal formulation.RESULTS:Third order regression equation gained made the good prediction about entrapment(85.4%)and alkanin load(5.16%),and exhibited no significant difference between theoretic value and measured value.CONCLUSION:The combination of orthogonal design with central composite design is a feasible and convenient method to optimize the prescription of alkanin-liposome.