CAJ | 학술논문

Objective:This study was to investigate the variables in bone marrow harvesting procedure and individual donor factors which can potentially affect the yield of mesenchymal stromal cells(MSC).Methods:We determined the yield of MSC from bone marrow under different clinical conditions by comparing the MSC colony numbers from:(1)donors of different ages;(2)healthy donors and patients with leukemia;(3)bone marrow aspirated at different time points during marrow harvesting;(4)bone marrow harvested by different needles.Results:During the process of harvesting,the number of MSC significantly decreased with increase number of aspiration,from 675/ml at the initial decreased to 60/ml after 100 ml bone marrow aspirated,and 50/ml after 200ml bone marrow aspirated.The number of MSC retrieved from leukemia patients(99/m1 bone marrow)was significantly lower than that of healthy donors(708/ml bone marrow).However,there was no significant difference in growth rate.There was no significant age-related difference of MSC yielded from donors <55 years.And there was no significant difference in MSC number between the samples from single end-holed needle and those from multiple-side-hole needle.Conclusion:The optimal bone marrow samples for MSC collection should be obtained earlier in the process of harvesting procedure.Bone marrow from donors <55 years was equally good as MSC sources.The autologous MSC from leukemia patients can be utilized for in-vitro MSC expansion.