CAJ | 학술논문

目的探讨阿托伐他汀(atorvastatin)对病毒性心肌炎(VMC)小鼠心脏组织及功能的影响.方法 4周龄近交系纯种Balb/c小鼠146只随机(随机数字法)分为4组:(1)健康组(n=18)；(2)VMC组(n=60),腹腔接种柯萨奇病毒；(3)对照组(n=18),只给予阿托伐他汀；(4)VMC药物治疗组(n=50),给予腹腔接种柯萨奇病毒,并给以阿托伐他汀连续治疗用药2周.接种病毒后3,7,10,14,21,30 d,检测超声心动图、收集血样检测血清cTnI水平,HE染色观察心肌组织炎症浸润程度,电镜观察心肌胶原纤维、心肌细胞及各种细胞器的变化.资料组间比较采用方差分析,post-hoc检验用于比较各组生存间的差异.结果 VMC组比VMC药物治疗组的30 d累计生存率低(59.2％ vs.87.0％),差异具有统计学意义(P=0.008).VMC药物冶疗组与VMC组比较,在第10,14,21,30天的心肌病理组织学积分均降低,差异具有统计学意义(P＜0.05).VMC药物治疗组比VMC组心肌损伤的病灶数量少,心肌线粒体和肌浆网改变也较同期VMC组轻.腹腔注射病毒后第7天与对照组比较,VMC组小鼠出现心功能减低[(69.82±5.12)vs.(89.23±2.01),P＜0.01].与VMC组比较,VMC药物治疗组EF值明显增高[(78.99±5.23)vs.(69.82±5.12),P＜0.01]；与VMC组比较,VMC药物治疗组的cTnI值明显下降.结论阿托伐他汀改善VMC小鼠生存率,改善组织病理学表现,减少心肌受损程度,提高心脏功能.阿托伐他汀可能是一种潜在的治疗VMC的方法.
목적 탐토아탁벌타정(atorvastatin)대병독성심기염(VMC)소서심장조직급공능적영향.방법 4주령근교계순충Balb/c소서146지수궤(수궤수자법)분위4조:(1)건강조(n=18)；(2)VMC조(n=60),복강접충가살기병독；(3)대조조(n=18),지급여아탁벌타정；(4)VMC약물치료조(n=50),급여복강접충가살기병독,병급이아탁벌타정련속치료용약2주.접충병독후3,7,10,14,21,30 d,검측초성심동도、수집혈양검측혈청cTnI수평,HE염색관찰심기조직염증침윤정도,전경관찰심기효원섬유、심기세포급각충세포기적변화.자료조간비교채용방차분석,post-hoc검험용우비교각조생존간적차이.결과 VMC조비VMC약물치료조적30 d루계생존솔저(59.2％ vs.87.0％),차이구유통계학의의(P=0.008).VMC약물야료조여VMC조비교,재제10,14,21,30천적심기병리조직학적분균강저,차이구유통계학의의(P＜0.05).VMC약물치료조비VMC조심기손상적병조수량소,심기선립체화기장망개변야교동기VMC조경.복강주사병독후제7천여대조조비교,VMC조소서출현심공능감저[(69.82±5.12)vs.(89.23±2.01),P＜0.01].여VMC조비교,VMC약물치료조EF치명현증고[(78.99±5.23)vs.(69.82±5.12),P＜0.01]；여VMC조비교,VMC약물치료조적cTnI치명현하강.결론 아탁벌타정개선VMC소서생존솔,개선조직병이학표현,감소심기수손정도,제고심장공능.아탁벌타정가능시일충잠재적치료VMC적방법.
Objective To investigate the effects of atorvastatin on the improvement of cardiac function of mice with myocarditis.Methods A total of 146 Balb/c mice were divided into four groups randomly(random number).The viral myocarditis(VMC)model was made by Coxsakie virus B3(CVB)injected intra-abdominally.Four groups were normal group(n =18),VMC group(n =60),Control group (n=18)and VMCtreatment group(n =50).The mice of control group were treated with atorvastatin without VMC,and the mice of VMC treatment group were with VMC and were given atorvastatin for 2 weeks.Echocardiograms were used 3,7,10,14,21,and 30 days after virus inoculation.Blood samples were collected for cardiac troponin-Ⅰ detection at the same time.Myocardial inflammation was examined by using histochemistry staining.The changes of myocardial collagen fiber,myocardial cells and various organelles were examined by electron microscope.Results Compared with VMC group,the cumulative survival rate of VMC group treatment group was higher(87.0％ vs 59.2％)after treatment with atorvastatin for 30 days (P =0.008),and the improvement of pathological features after treatment with atorvastatin was found 10,14,21 and 30 days after the inoculation.Compared with control group,the cardiac function was decreased in the CVB infected mice 7 days after virus challenge[(69.82 ±5.12)vs(89.23 ±2.01),P ＜0.01]and compared with VMC group,the EF values of VMC treatment group were significantly higher 7,14,21and 30 days after virus inoculation.The differences in cTnI values between VMC group and CVB treatment group were statistically significant 7,10,14 and 21 days after virus challenge.Conclusions These results demonstrate that atorvastatin improves survival rates and the histological features in CVB3m-induced myocarditis.It can improve the heart function of CVB infected mice.Atorvastatin could be a treatment of choice for VMC.