中华胰腺病杂志
中華胰腺病雜誌
중화이선병잡지
CHINESE JOURNAL OF PANCREATOLOGY
2010年
3期
184-186
,共3页
胰腺炎%细胞凋亡%新癀片%Bcl-2%c-Myc
胰腺炎%細胞凋亡%新癀片%Bcl-2%c-Myc
이선염%세포조망%신황편%Bcl-2%c-Myc
Pancereatitis%Apoptosis%Xinhuang Pian%Bcl-2%c-Myc
目的 观察新癀片对急性胰腺炎(AP)大鼠胰腺细胞凋亡和胰腺组织Bcl-2、c-Myc蛋白表达的影响,并探讨其作用机制.方法 90只Wistar大鼠按完全随机法分为对照组、AP组和新癀片治疗组.采用胆胰管逆行注射5%牛黄胆酸钠1 ml/kg体重方法制备AP模型.治疗组于制模后30 min按480 mg/kg体重的剂量予新癀片水溶液灌胃.术后6、12、24 h分批处死大鼠,检测血淀粉酶活性,观察胰腺病理变化,TUNEL法检测胰腺细胞凋亡,Western blotting法检测胰腺组织Bcl-2、c-Myc蛋白表达.结果 对照组12 h点的血淀粉酶活性、胰腺病理评分、凋亡指数、胰腺组织Bcl-2和c-Myc蛋白表达量分别为(1927±186)U/L、0、0.59±0.12、0.406±0.112、0.185±0.046;AP组分别为(5611±473)U/L、2.65±0.56、3.80±0.91、0.282±0.082、0.339±0.076;治疗组分别为(4572±400)U/L、2.40±0.75、7.15±0.86、0.220±0.068、0.302±0.090.AP组和治疗组的淀粉酶活性、胰腺病理评分、凋亡指数、c-Myc蛋白表达量均显著高于对照组(P值均＜0.05),而Bcl-2蛋白表达量显著低于对照组(P＜0.05).与AP组比较,治疗组的淀粉酶活性、胰腺病理评分、胰腺组织Bcl-2蛋白表达量均显著降低(P值均＜0.05),凋亡指数显著增高(P＜0.05),c-Myc蛋白表达量无显著变化.结论 新癀片可通过下调Bcl-2蛋白表达、诱导AP早期胰腺细胞凋亡,从而减轻胰腺炎症反应.
目的 觀察新癀片對急性胰腺炎(AP)大鼠胰腺細胞凋亡和胰腺組織Bcl-2、c-Myc蛋白錶達的影響,併探討其作用機製.方法 90隻Wistar大鼠按完全隨機法分為對照組、AP組和新癀片治療組.採用膽胰管逆行註射5%牛黃膽痠鈉1 ml/kg體重方法製備AP模型.治療組于製模後30 min按480 mg/kg體重的劑量予新癀片水溶液灌胃.術後6、12、24 h分批處死大鼠,檢測血澱粉酶活性,觀察胰腺病理變化,TUNEL法檢測胰腺細胞凋亡,Western blotting法檢測胰腺組織Bcl-2、c-Myc蛋白錶達.結果 對照組12 h點的血澱粉酶活性、胰腺病理評分、凋亡指數、胰腺組織Bcl-2和c-Myc蛋白錶達量分彆為(1927±186)U/L、0、0.59±0.12、0.406±0.112、0.185±0.046;AP組分彆為(5611±473)U/L、2.65±0.56、3.80±0.91、0.282±0.082、0.339±0.076;治療組分彆為(4572±400)U/L、2.40±0.75、7.15±0.86、0.220±0.068、0.302±0.090.AP組和治療組的澱粉酶活性、胰腺病理評分、凋亡指數、c-Myc蛋白錶達量均顯著高于對照組(P值均＜0.05),而Bcl-2蛋白錶達量顯著低于對照組(P＜0.05).與AP組比較,治療組的澱粉酶活性、胰腺病理評分、胰腺組織Bcl-2蛋白錶達量均顯著降低(P值均＜0.05),凋亡指數顯著增高(P＜0.05),c-Myc蛋白錶達量無顯著變化.結論 新癀片可通過下調Bcl-2蛋白錶達、誘導AP早期胰腺細胞凋亡,從而減輕胰腺炎癥反應.
목적 관찰신황편대급성이선염(AP)대서이선세포조망화이선조직Bcl-2、c-Myc단백표체적영향,병탐토기작용궤제.방법 90지Wistar대서안완전수궤법분위대조조、AP조화신황편치료조.채용담이관역행주사5%우황담산납1 ml/kg체중방법제비AP모형.치료조우제모후30 min안480 mg/kg체중적제량여신황편수용액관위.술후6、12、24 h분비처사대서,검측혈정분매활성,관찰이선병리변화,TUNEL법검측이선세포조망,Western blotting법검측이선조직Bcl-2、c-Myc단백표체.결과 대조조12 h점적혈정분매활성、이선병리평분、조망지수、이선조직Bcl-2화c-Myc단백표체량분별위(1927±186)U/L、0、0.59±0.12、0.406±0.112、0.185±0.046;AP조분별위(5611±473)U/L、2.65±0.56、3.80±0.91、0.282±0.082、0.339±0.076;치료조분별위(4572±400)U/L、2.40±0.75、7.15±0.86、0.220±0.068、0.302±0.090.AP조화치료조적정분매활성、이선병리평분、조망지수、c-Myc단백표체량균현저고우대조조(P치균＜0.05),이Bcl-2단백표체량현저저우대조조(P＜0.05).여AP조비교,치료조적정분매활성、이선병리평분、이선조직Bcl-2단백표체량균현저강저(P치균＜0.05),조망지수현저증고(P＜0.05),c-Myc단백표체량무현저변화.결론 신황편가통과하조Bcl-2단백표체、유도AP조기이선세포조망,종이감경이선염증반응.
Objective To investigate the effect of Xinhuang Pian on pancreatic apoptosis and the protein expression of Bcl-2 and c-Myc in rat with acute pancreatitis (AP). Methods 90 Wister rats were randomly divided into 3 groups, including control group, AP group and Xinhuang Pian treatment (XP) group.AP model was induced by pancreaticobiliary injection of 5% sodium taurocholate (1ml/kg weight). Rats in XP group were given Xinhuang Pian solution (480 mg/kg weight) lavage 30min after AP induction, The rats were sacrificed at 6, 12, 24 h after AP induction, and serum amylase levels were determed, pancreatic pathological change was observed; apoptosis was determined by TUNEL method. The determination of Bcl-2and c-Myc protein expression was performed by Western blot. Results 12 h after AP induction, serum amylase, pancreatic pathological scores, apoptotic indexes and the protein expression of Bcl-2 and c-Myc were (1927 ± 186) U/L, 0, 0.59 ± 0. 12, 0.406 ± 0. 112, 0. 185 ± 0. 046 in control group; (5611 ± 473) U/L,2.65 ± 0. 56, 3.80 ± 0. 91, 0. 282 ± 0.082, 0. 339 ± 0. 076 in AP group; and (4572 ± 400) U/L, 2.40 ± 0. 75,7.15 ± 0. 86, 0. 220 ± 0.068, 0. 302 ± 0. 090 in XP group. The levels of serum amylase, pancreatic pathological scores, apoptotic indexes and the protein expression of c-myc in AP and XP group were all significantly higher than those in control group (P < 0. 05), while the protein expression of Bcl-2 was significanfiy lower than that in control group ( P < 0.05 ). Compared with AP group, the levels of serum amylase, pancreatic pathological scores, protein expression of Bcl-2 were all significanfiy lower in XP group (P < 0. 05 ), but the apoptotic indexe was significantly higher (P < 0. 05 ), and there was no significat change of expression of c-Myc protein.Conclusions Xinhuang Pian may induce the pancreatic apoptosis through down-regulating Bcl-2 protein expression, and result in the alleviation of pancreatic inflammatory reaction.
