中华糖尿病杂志
中華糖尿病雜誌
중화당뇨병잡지
CHINES JOURNAL OF DLABETES MELLITUS
2010年
5期
344-348
,共5页
类泛素样蛋白%基因多态性%2型糖尿病
類汎素樣蛋白%基因多態性%2型糖尿病
류범소양단백%기인다태성%2형당뇨병
SUMO4%Polymorphism%Type 2 diabetes
目的 研究湖北地区汉族人群中类泛素样蛋白4(SUMO4)M55V基因多态性与2型糖尿病及其相关因素的关系.方法 采用聚合酶链反应-限制性片段长度多态性分析(PCR-RFLP)法对湖北地区2006年2月至2009年11月在武汉大学中南医院门诊及病房新诊断的427例2型糖尿病患者(糖尿病组)及281名健康对照者(对照组)进行SUMO4 M55V基因多态性检测,空腹测量身高、体重、腰围、血压,测定血糖、胰岛素、糖化血红蛋白(HbA1c)及甘油三酯(TG)等各项指标,并进一步探讨2型糖尿病及其相关危险因素和SUMO4 M55V基因多态性的关系.统计学方法采用Logistic多元回归分析评估与2型糖尿病发生相关的危险因素.结果 糖尿病组的腰围(t=2.72,P＜0.01)、体重指数(BMI,t=2.66,P＜0.01)、空腹血糖(t=2.81,P＜0.01)、餐后血糖(t=3.07,P＜0.01)、HbA1c(t=2.79,P＜0.01)和甘油三酯(t=2.63,P＜0.01)水平明显高于对照组.糖尿病组和对照组相比SUMO4 M55V的GG基因型分布频率显著升高(3个基因型比较:x2=6.782,P＜0.05),且在人群中,携带GG基因型的患病风险是AA基因型的2倍(OR=2.011).Logistic多元回归分析显示,HbA1c(P≤0.05,OR=2.57,95%CI:1.92～4.63)、体重(P＜0.05,OR=2.61,95%CI:1.74～4.37)、SUMO4M55V基因多态性(P＜0.05,OR=1.47,95%CI:1.09～3.42)和TG(P＜0.05,OR=1.59,95%CI:1.24～4.63)与2型糖尿病的发生明显相关.进一步对2型糖尿病不同基因型的HbA1c、BMI、TG、空腹胰岛素和胰岛素抵抗水平进行分析,发现在3个基因型之间TG差异明显(F=3.382,P＜0.05),与AA基因型相比AG和GG基因型患者的空腹胰岛素(F=4.075,P＜0.05)和胰岛素抵抗指数(HOMA-IR)(F=14.389,P＜0.001)的水平也更高.结论 在湖北地区汉族人群中,SUMO4 M55V基因多态性和2型糖尿病发生风险相关.
目的 研究湖北地區漢族人群中類汎素樣蛋白4(SUMO4)M55V基因多態性與2型糖尿病及其相關因素的關繫.方法 採用聚閤酶鏈反應-限製性片段長度多態性分析(PCR-RFLP)法對湖北地區2006年2月至2009年11月在武漢大學中南醫院門診及病房新診斷的427例2型糖尿病患者(糖尿病組)及281名健康對照者(對照組)進行SUMO4 M55V基因多態性檢測,空腹測量身高、體重、腰圍、血壓,測定血糖、胰島素、糖化血紅蛋白(HbA1c)及甘油三酯(TG)等各項指標,併進一步探討2型糖尿病及其相關危險因素和SUMO4 M55V基因多態性的關繫.統計學方法採用Logistic多元迴歸分析評估與2型糖尿病髮生相關的危險因素.結果 糖尿病組的腰圍(t=2.72,P＜0.01)、體重指數(BMI,t=2.66,P＜0.01)、空腹血糖(t=2.81,P＜0.01)、餐後血糖(t=3.07,P＜0.01)、HbA1c(t=2.79,P＜0.01)和甘油三酯(t=2.63,P＜0.01)水平明顯高于對照組.糖尿病組和對照組相比SUMO4 M55V的GG基因型分佈頻率顯著升高(3箇基因型比較:x2=6.782,P＜0.05),且在人群中,攜帶GG基因型的患病風險是AA基因型的2倍(OR=2.011).Logistic多元迴歸分析顯示,HbA1c(P≤0.05,OR=2.57,95%CI:1.92～4.63)、體重(P＜0.05,OR=2.61,95%CI:1.74～4.37)、SUMO4M55V基因多態性(P＜0.05,OR=1.47,95%CI:1.09～3.42)和TG(P＜0.05,OR=1.59,95%CI:1.24～4.63)與2型糖尿病的髮生明顯相關.進一步對2型糖尿病不同基因型的HbA1c、BMI、TG、空腹胰島素和胰島素牴抗水平進行分析,髮現在3箇基因型之間TG差異明顯(F=3.382,P＜0.05),與AA基因型相比AG和GG基因型患者的空腹胰島素(F=4.075,P＜0.05)和胰島素牴抗指數(HOMA-IR)(F=14.389,P＜0.001)的水平也更高.結論 在湖北地區漢族人群中,SUMO4 M55V基因多態性和2型糖尿病髮生風險相關.
목적 연구호북지구한족인군중류범소양단백4(SUMO4)M55V기인다태성여2형당뇨병급기상관인소적관계.방법 채용취합매련반응-한제성편단장도다태성분석(PCR-RFLP)법대호북지구2006년2월지2009년11월재무한대학중남의원문진급병방신진단적427례2형당뇨병환자(당뇨병조)급281명건강대조자(대조조)진행SUMO4 M55V기인다태성검측,공복측량신고、체중、요위、혈압,측정혈당、이도소、당화혈홍단백(HbA1c)급감유삼지(TG)등각항지표,병진일보탐토2형당뇨병급기상관위험인소화SUMO4 M55V기인다태성적관계.통계학방법채용Logistic다원회귀분석평고여2형당뇨병발생상관적위험인소.결과 당뇨병조적요위(t=2.72,P＜0.01)、체중지수(BMI,t=2.66,P＜0.01)、공복혈당(t=2.81,P＜0.01)、찬후혈당(t=3.07,P＜0.01)、HbA1c(t=2.79,P＜0.01)화감유삼지(t=2.63,P＜0.01)수평명현고우대조조.당뇨병조화대조조상비SUMO4 M55V적GG기인형분포빈솔현저승고(3개기인형비교:x2=6.782,P＜0.05),차재인군중,휴대GG기인형적환병풍험시AA기인형적2배(OR=2.011).Logistic다원회귀분석현시,HbA1c(P≤0.05,OR=2.57,95%CI:1.92～4.63)、체중(P＜0.05,OR=2.61,95%CI:1.74～4.37)、SUMO4M55V기인다태성(P＜0.05,OR=1.47,95%CI:1.09～3.42)화TG(P＜0.05,OR=1.59,95%CI:1.24～4.63)여2형당뇨병적발생명현상관.진일보대2형당뇨병불동기인형적HbA1c、BMI、TG、공복이도소화이도소저항수평진행분석,발현재3개기인형지간TG차이명현(F=3.382,P＜0.05),여AA기인형상비AG화GG기인형환자적공복이도소(F=4.075,P＜0.05)화이도소저항지수(HOMA-IR)(F=14.389,P＜0.001)적수평야경고.결론 재호북지구한족인군중,SUMO4 M55V기인다태성화2형당뇨병발생풍험상관.
Objective To investigate the relationship between small ubiquitin-like modifer 4 M55V polymorphism with type 2 diabetes and related factors. Methods We analyzed the SUMO4 M55V polymorphism of 427 newly diagnosed type 2 diabetic patients, who were diagnosed firstly at outpatient and inpatient in Zhongnan Hospital of Wuhan University from 2006 February to 2009 September, with 281 healthy controls, by PCR-RFLP assay, and gathered their body height, weight, waistline, blood pressure on an empty stomach. Blood glucose, insulin and HbA1c also measured. Then analyzed the relationship between SUMO4 M55V polymorphism with type 2 diabetes and its related factors, with using logistic analysis for evaluating the risk factors of type 2 diabetes. Results Compared with the control group, the levels of waist circumference(t =2.72, P＜0.01), BMI(t=2.66, P＜0.01), FBG(t =2.81, P＜0. 01), PPBG (t =3.07, P＜0. 01)HbA1c(t =2.79, P＜0.01) and TG(t =2.63, P＜0. 01) are significantly higher in T2DM group( P ＜0. 01 ). The frequency of GG genotype was significantly higher in T2DM group( compare with the three genotypes: x2 =6. 782,P ＜0. 05), and the risk of type 2 diabetes is two times higher in GG genotype compared with AA genotype ( OR =2. 011 ). Logistic multifactor analysis found that there is notable association between type 2 diabetes incidence with HbA1 c ( P ＜ 0. 05, OR= 2. 57,95% CI: 1.92 - 4. 63),body weight(P ＜0.05,OR=2.61, 95%CI: 1.74-4.37), SUMO4 M55V polymorphism (P ＜0.05,OR = 1.47,95% CI: 1.09 - 3.42), TG( P ＜ 0. 05, OR = 1.59,95% CI: 1.24 - 4. 63 ). Further analysis of HbA1c, BMI, TG, FINS and HOMA-IR showed that there was significant difference of TG among different genotypes( F = 3. 382, P ＜0. 05), and compared with AA genotype, levels of FINS( F =4. 075, P ＜0. 05 )and HOMA-IR(F= 14.389,P＜0.001) in AG and GG were also higher. Conclusion SUMO4 M55V polymorphism is associated with the incidence of type 2 diabetes among the Han population in Hubei.
