CAJ | 학술논문

目的戊型肝炎病毒的人兽共患特征被认可,本研究检测华东地区猪群在不同季节中基因4型戊肝病毒(HEV)的感染情况,了解猪病毒株之间以及与人病毒株的同源性关系.方法2007年9月-2008年6月在华东地区浙江、安徽和江苏的3家屠宰场中共采集猪胆汁标本1200份,应用巢式RT-PCR方法检测HEV RNA,对阳性标本测序并结合同一地区人HEV序列进行同源性分析.结果猪胆汁标本中HEV RNA总检出率为4.5%,各季节猪群的检出率为9-10月平均检出率6%,12-1月4.33%,3-4月4.33%,5-6月3.33%,各地区的平均检出率为江苏6%、安徽5%、浙江2.5%.不同地区猪与猪、猪与人基因4型HEV病毒株的同源性都较高(猪株之间核苷酸序列约为80%～100%,氨基酸序列约为96%～100%;猪与人株之间核苷酸序列约为76%～99%,氨基酸序列约为95%～100%).部分猪HEV与人HEV构成独立的同源性分支,进化起源关系密切.结论基因4型HEV在华东地区猪群中常年广泛流行,且可能拥有共同的进化与传播起源.猪群携带HEV可能会对人群戊肝的流行趋势产生影响.
목적 무형간염병독적인수공환특정피인가,본연구검측화동지구저군재불동계절중기인4형무간병독(HEV)적감염정황,료해저병독주지간이급여인병독주적동원성관계.방법2007년9월-2008년6월재화동지구절강、안휘화강소적3가도재장중공채집저담즙표본1200빈,응용소식RT-PCR방법검측HEV RNA,대양성표본측서병결합동일지구인HEV서렬진행동원성분석.결과저담즙표본중HEV RNA총검출솔위4.5%,각계절저군적검출솔위9-10월평균검출솔6%,12-1월4.33%,3-4월4.33%,5-6월3.33%,각지구적평균검출솔위강소6%、안휘5%、절강2.5%.불동지구저여저、저여인기인4형HEV병독주적동원성도교고(저주지간핵감산서렬약위80%～100%,안기산서렬약위96%～100%;저여인주지간핵감산서렬약위76%～99%,안기산서렬약위95%～100%).부분저HEV여인HEV구성독립적동원성분지,진화기원관계밀절.결론 기인4형HEV재화동지구저군중상년엄범류행,차가능옹유공동적진화여전파기원.저군휴대HEV가능회대인군무간적류행추세산생영향.
Objective To determine the seasonal prevalence of genotype-Ⅳ hepatitis E virus (HEV) in swine herds in Eastern China and explore the phylogenetic relationship between swine HEV and human HEV in the situation that zoonotic features of HEV had been confirmed. Methods From September 2007 to June 2008 ,a total of 1200 swine bile specimens were collected from three slaughter houses located in Zhejiang,Anhui and Jiangsu, the Eastern China, and detected for HEV RNA by using nested RT-PCR. The positive PCR products were sequenced. Then the swine HEV were phylogenetically determined with human HEV isolated in Eastern China. Results The positive rate for HEV RNA in swine herds was 4.5% totally. Significant differences of HEV detection were not observed among seasonal pattern (Sep-Oct: 6%, Dec-Jan: 4.33% ,Max-Apr: 4.33% ,May-Jun: 3.33% ) but in geographic distribution (Jiangsu: 6%, Anhui: 5% ,Zhejiang: 2.5% ). Regardless of isolation from different areas,swine and human genotype-Ⅳ HEV shared a high similarity. Phylogenetically,there were 80%-100% and 96%-100% identities within swine genotype-Ⅳ HEV at the nucleotide and amino acid levels respectively. Between swine HEV and human HEV,there were also similarities of 76%-99% and 95%-100%. It was noted that some human and swine isolates were clustered with bootstrap values of>90%. Conclusion Genotype-Ⅳ HEV is widely prevalent in swine herds in Eastern China and original common ancestor of evolution and transmission was implied. The sustaining prevalence within swine herds should have a probable influence on the epidemic situation of hepatitis E in human beings.