CAJ | 학술논문

背景:祛瘀宁痛贴是第三军医大学大坪医院研制的中药外用制剂,由多种抗炎、镇痛药材组成,其对关节、肌肉、软组织疼痛具有较好的缓解作用.目的:研究祛瘀宁痛贴对组织肿胀和镇痛的作用及其机制.设计:随机对照实验研究.地点和对象:解放军第三军医大学药理学教研室.昆明种小鼠100只,体质量18～22 g,雌雄兼用,均由第三军医大学实验动物中心提供.干预:按随机数字表法等分5组,赋形剂对照组、吲哚美辛(消炎痛)组、祛瘀宁痛贴0.4 g/kg组、1.2 g/kg组和2.4 g/kg组.应用小鼠巴豆油性耳肿胀模型,观察本品的抗炎作用;采用电刺激致小鼠尖叫,研究其镇痛作用.主要观察指标:①应用祛瘀宁痛贴后小鼠耳肿胀度.②用药前后不同时间痛阈值.结果:用药后小鼠耳肿胀度,赋形剂对照组、吲哚美辛(消炎痛)组、祛瘀宁痛贴0.4 g/kg组、1.2 g/kg组和2.4 g/kg组分别为17.36±3.48,10.29±3.25,12.81±4.92,9.54±3.61,8.82±3.87.祛瘀宁痛贴与赋形剂对照组比较,显著抑制小鼠巴豆油性耳肿胀(t=2.384～5.176,P＜0.05～0.01),明显延长电刺激致小鼠尖叫的潜伏期(t=2.313～8.951,P＜0.05～0.01).结论:祛瘀宁痛贴对软组织肿胀及疼痛具有较好的抑制作用.
배경:거어저통첩시제삼군의대학대평의원연제적중약외용제제,유다충항염、진통약재조성,기대관절、기육、연조직동통구유교호적완해작용.목적:연구거어저통첩대조직종창화진통적작용급기궤제.설계:수궤대조실험연구.지점화대상:해방군제삼군의대학약이학교연실.곤명충소서100지,체질량18～22 g,자웅겸용,균유제삼군의대학실험동물중심제공.간예:안수궤수자표법등분5조,부형제대조조、신타미신(소염통)조、거어저통첩0.4 g/kg조、1.2 g/kg조화2.4 g/kg조.응용소서파두유성이종창모형,관찰본품적항염작용;채용전자격치소서첨규,연구기진통작용.주요관찰지표:①응용거어저통첩후소서이종창도.②용약전후불동시간통역치.결과:용약후소서이종창도,부형제대조조、신타미신(소염통)조、거어저통첩0.4 g/kg조、1.2 g/kg조화2.4 g/kg조분별위17.36±3.48,10.29±3.25,12.81±4.92,9.54±3.61,8.82±3.87.거어저통첩여부형제대조조비교,현저억제소서파두유성이종창(t=2.384～5.176,P＜0.05～0.01),명현연장전자격치소서첨규적잠복기(t=2.313～8.951,P＜0.05～0.01).결론:거어저통첩대연조직종창급동통구유교호적억제작용.
BACKGROUND: Quyu ningtong tie(QYNT) is Chinese herbal topical developed and prepared by Daping Hospital affiliated to No. 3 Military Medical University, composed of various herbs for anti-inflammation and analgesia. It served a satisfactory releasing action on pain of joints, muscles and soft tissues.OBJECTIVE: To explore the inhibition of QYNT on swelling and analgesia in tissues.DESIGN: Randomized, controlled trial design.SETTING and PARTICIPANTS: The experiment was performed in the Department of Pharmacology, Third Military Medical University of Chinese PLA. Oue hundred Kunming mice of either sex, weighted varied from 18 to 22 g were employed, provided by Experimental Animal Center of Third Military Medical University of Chinese PLA.INTERVENTIONS: According to random number table, five groups were divided, i. e. excipient control group, indomethacin group, QYNT 0. 4 g/kg group, 1.2 g/kg group and 2.4 g/kg group. The model of auricular swelling in mice was prepared by croton oil and the product was applied to observe its action on anti-inflammation. The electric stimulation was adopted to induce screaming in mice and to explore the analgesia action.before and after medication.RESULTS: After medication, the values of 17.36 ± 3.48, 10. 29 ± 3.25,12. 81 ± 4.92,9.54 ± 3.61,8.82 ± 3.87 were presented in excipient control group, indomethacin group, QYNT 0. 4 g/kg group, 1.2 g/kg group and 2.4 g/kg group successively. Compared with excipient control, QYNT inhibited significantly croton oil auricular swelling in mice(t=2.384-5. 176, P ＜ 0.05 - 0.01 ), and prolonged remarkably the latent duration of screaming caused by electric stimulation( t = 2. 313 - 8.951, P ＜ 0.05 -0.0l).CONCLUSION: QYNT acts on satisfactory inhibition on swelling and pain of soft tissues.